{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:27:05Z","timestamp":1782228425646,"version":"3.54.5"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>\n                    Elastase, a serine protease, has been implicated in chronic obstructive pulmonary disease and systemic inflammatory response syndrome. In this study, we evaluated the effects of phillyrin and phillygenin, 2 major\n                    <jats:italic>Forsythia<\/jats:italic>\n                    lignans, on elastase inhibition. Both compounds exhibited competitive inhibition, as confirmed by enzymatic kinetics, spectroscopy, and molecular docking. Phillygenin exhibited stronger activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    0.5 mmol\/L,\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    4.0 \u00d7 10\n                    <jats:sup>\u22124<\/jats:sup>\n                    mol\/L) than phillyrin (IC\n                    <jats:sub>50<\/jats:sub>\n                    1.5 mmol\/L,\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    9.7 \u00d7 10\n                    <jats:sup>\u22124<\/jats:sup>\n                    mol\/L), likely due to reduced steric hindrance. Spectroscopic analysis revealed ligand-induced conformational changes in elastase, characterized by increased \u03b1-helix and random coil content and decreased \u03b2-sheet structures. Docking revealed interactions involving \u03c0-cation, \u03c0-sigma, hydrogen bonds, hydrophobic forces, electrostatics, and van der Waals effects. These results provide mechanistic insights into the inhibitory effects of phillyrin and phillygenin and highlight their potential as therapeutic agents for elastase-related diseases.\n                  <\/jats:p>","DOI":"10.3389\/fnut.2026.1723757","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T06:51:30Z","timestamp":1770792690000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative inhibitory effects of phillyrin and phillygenin on elastase: mechanisms and therapeutic potential"],"prefix":"10.3389","volume":"13","author":[{"given":"Wenhui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, The Second Hospital of Tianjin Medical University","place":["Tianjin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Fu","sequence":"additional","affiliation":[{"name":"College of Chemistry, Changchun Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliu","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Chemistry, Changchun Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxue","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingang","family":"Mo","sequence":"additional","affiliation":[{"name":"School of Life Science, Changchun Normal University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The First Hospital of Jilin University","place":["Changchun, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.3390\/antiox11091683","volume":"11","author":"Shin","year":"2022","journal-title":"Camellia sinensisAntioxidants"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.3390\/biom11081065","article-title":"Neutrophil elastase and chronic lung 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