{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T15:00:38Z","timestamp":1774882838868,"version":"3.50.1"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Resin glycosides (RGs) found in the aerial parts of sweet potato are health promoting agents that can be reclaimed from the by-products of sweet potato plantation as agriceuticals for combating obesity. However, RGs have complex structural variations across various sweet potato cultivars, along with an unclear structure\u2013activity relationship. To address this gap, nine types of sweet potato leaves were evaluated, including \u201cShangshu 19\u201d, \u201cXuzi 20-1\u201d, \u201cXushu 24\u201d, \u201cXushu 32\u201d, \u201cYanshu 25\u201d, \u201cBlackheart\u201d, \u201cBlackie\u201d, and two \u201cBeniazuma\u201d samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RG-rich extracts were obtained from sweet potato leaves by using dichloromethane extraction. Pancreatic lipase (PL) inhibitory activity was then determined by a p-nitrophenyl palmitate (pNPP)-based assay. Individual RG profiles were characterized and semi-quantified by UHPLC\u2013Q-TOF-MS\/MS, followed by multivariate analysis to evaluate cultivar-dependent variations and identify spectral features associated with PL inhibition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Xushu 32 and Blackheart cultivars exhibited the highest PL inhibitory activities with an Orlistat equivalent (OE) of 2.44 \u00b1 0.30 and 2.02 \u00b1 0.10 ng orlistat\/\u03bcg extracts, respectively. In total, 128 RGs were tentatively identified based on UHPLC\u2013Q-TOF-MS\/MS analysis. Multivariate analysis revealed cultivar-dependent differences in RG profiles and identified four RGs as potential marker compounds associated with PL inhibition. All four RGs were pentasaccharides featuring 2-methylbutyric and dodecanoic acid as side chains. Further mechanistic study revealed that long-chain fatty acid esters or a macrocyclic lactone moiety on the RG backbone may play a key role in conferring inhibition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>Taken together, these findings may serve as valuable guidelines for foodomic analysis of RGs and cultivar selection in developing anti-obesity functional foods derived from agricultural by-products.In addition, we would like to double-check that the formatting of Table 1 is maintained as in the original manuscript we provided. The current proof version includes additional internal table lines that slightly alter the intended meaning of the table.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnut.2026.1783378","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T06:48:29Z","timestamp":1773902909000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["UHPLC-Q-TOF-MS\/MS profiling of resin glycosides and their lipase inhibitory activity in leaves of selected sweet potato (Ipomoea batatas L.) cultivars"],"prefix":"10.3389","volume":"13","author":[{"given":"Yi","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixuan","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingtong","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuyun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne Yi Hui","family":"Toy","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dejian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, National University of Singapore","place":["Singapore, Singapore"]},{"name":"National University of Singapore (Suzhou) Research Institute","place":["Suzhou, Jiangsu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1007\/s11418-017-1114-5","article-title":"Resin glycosides from Convolvulaceae plants","volume":"71","author":"Ono","year":"2017","journal-title":"J Nat Med"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"2025","DOI":"10.1002\/med.21916","article-title":"Chemistry and biological activity of resin glycosides from Convolvulaceae species","volume":"42","author":"Fan","year":"2022","journal-title":"Med Res Rev"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"5353","DOI":"10.1039\/D2FO00555G","article-title":"Resin glycosides in aerial parts of Ipomoea Batatas are potent lipase inhibitors: potential upcycling of sweet potato by-products to combat obesity","volume":"13","author":"Toy","year":"2022","journal-title":"Food Funct"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"111552","DOI":"10.1016\/j.foodres.2022.111552","article-title":"Resin glycoside extracts from Ipomoea Aquatica retard lipid digestibility of high-fat food in vitro","volume":"159","author":"Toy","year":"2022","journal-title":"Food Res Int"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"27(23)","DOI":"10.3390\/molecules27238161","article-title":"Resin glycosides from Convolvulaceae Family: an 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