{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:47:48Z","timestamp":1782712068240,"version":"3.54.5"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>\n                    Enzymatic transformation of lignocellulosic biomass offers an eco-friendly pathway to produce valuable\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -oligoglucosides. In this study, an acidophilic GH12 xyloglucanase (TaXEG12) from\n                    <jats:italic>Trichoderma asperellum<\/jats:italic>\n                    ND-1 was overexpressed in\n                    <jats:italic>Komagataella phaffii<\/jats:italic>\n                    . TaXEG12 displayed maximum activity at 40\u202f\u00b0C and a pH of 4.0, while retaining &amp;gt;80% activity in the presence of 1.71\u202fM NaCl and 10% ethanol. Glu\n                    <jats:sup>140<\/jats:sup>\n                    and Glu\n                    <jats:sup>227<\/jats:sup>\n                    are important catalytic residues, while Asp\n                    <jats:sup>126<\/jats:sup>\n                    plays an auxiliary role in xyloglucan degradation. The enzyme showed strict substrate specificity toward tamarind xyloglucan. Through fed-batch fermentation, the enzyme production of TaXEG12 improved by 28.5-fold (2914.5\u202f\u00b1\u202f65.7\u202fU\/mL). Interestingly, TLC and ESI-MS analyses revealed that TaXEG12 could hydrolyze xyloglucan into xyloglucan oligosaccharides (XXXG, XXLG\/XLXG, and XLLG). These xyloglucan oligosaccharides can promote the growth of\n                    <jats:italic>Lactobacillus bulgaricus<\/jats:italic>\n                    . Additionally, the synergy degrees of corn bran and apple pulp in the degradation by TaXEG12 and TrCel were 1.34 and 1.32, respectively. TaXEG12 displayed unique properties and could be a desirable catalyst for the more sustainable production of high-value oligosaccharides, which are significantly beneficial for human intestinal health.\n                  <\/jats:p>","DOI":"10.3389\/fnut.2026.1850050","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:39:14Z","timestamp":1782711554000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["An acidophilic GH12 xyloglucanase from Trichoderma asperellum produces prebiotic oligosaccharides that promote probiotic growth"],"prefix":"10.3389","volume":"13","author":[{"given":"Fengzhen","family":"Zheng","sequence":"first","affiliation":[{"name":"Zhejiang International Joint Laboratory on Low-Carbon Pollution Control and Resource Utilization, College of Biological and Environmental Engineering, Zhejiang Shuren University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang International Joint Laboratory on Low-Carbon Pollution Control and Resource Utilization, College of Biological and Environmental Engineering, Zhejiang Shuren University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sicheng","family":"Rong","sequence":"additional","affiliation":[{"name":"Zhejiang International Joint Laboratory on Low-Carbon Pollution Control and Resource Utilization, College of Biological and Environmental Engineering, Zhejiang Shuren University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of ENT and Head and Neck Surgery, The Children's Hospital Zhejiang University School of Medicine","place":["Zhejiang, Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University","place":["Baltimore, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Basit","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Jhang","place":["Jhang, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1195015","DOI":"10.3389\/fnut.2023.1195015","article-title":"Upcycling post-harvest biomass residues from native European Lupinus species: from straws and pod shells production to nutritive value and alkaloids content for ruminant animals","volume":"10","author":"Maia","year":"2023","journal-title":"Front Nutr"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"156310","DOI":"10.1016\/j.cej.2024.156310","article-title":"Bidirectional uptake and redistribution, bio-stimuli responsive xyloglucan-based nanodelivery system for enhanced translocation of non-systemic pesticide in soybean plants","volume":"500","author":"Xiong","year":"2024","journal-title":"Chem Eng J"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1016\/j.ijbiomac.2019.08.210","article-title":"Characterization of an alkali-stable xyloglucanase\/mixed-linkage \u03b2-glucanase Pgl5A from Paenibacillus sp. 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