{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:11:51Z","timestamp":1790205111730,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:p>\n                    Ulcerative colitis (UC) is a chronic inflammatory disease caused by intestinal immune dysregulation and microbial imbalance. Pasteurized\n                    <jats:italic>Akkermansia muciniphila<\/jats:italic>\n                    has attracted significant attention in recent research due to its anti-inflammatory properties and favorable safety profile. In this study, 39 strains of pasteurized\n                    <jats:italic>A. muciniphila<\/jats:italic>\n                    with anti-inflammatory properties were preliminarily screened using an\n                    <jats:italic>in vitro<\/jats:italic>\n                    HT-29 cell inflammation model. Subsequently, the\n                    <jats:italic>in vivo<\/jats:italic>\n                    efficacy of these strains was evaluated in DSS-induced UC mouse models. It is noteworthy that the 39 strains exhibited considerable variation in their efficacy in treating UC. Among them, pasteurized AKK2645 demonstrated the strongest therapeutic efficacy in both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Pasteurized AKK2645 altered the ratio of Th17\/Treg and Th1\/Th2 cells in the colon, thereby reducing the mRNA expression of pro-inflammatory cytokines such as\n                    <jats:italic>il-6<\/jats:italic>\n                    ,\n                    <jats:italic>il-1\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>tnf-\u03b1<\/jats:italic>\n                    ,\n                    <jats:italic>il-17a<\/jats:italic>\n                    ,\n                    <jats:italic>ifn-\u03b3<\/jats:italic>\n                    , and\n                    <jats:italic>il-23<\/jats:italic>\n                    , while simultaneously upregulating the mRNA expression of anti-inflammatory cytokines such as\n                    <jats:italic>il-10<\/jats:italic>\n                    ,\n                    <jats:italic>foxp3<\/jats:italic>\n                    , and\n                    <jats:italic>gata3<\/jats:italic>\n                    . Furthermore, 16S rRNA analysis revealed that pasteurized AKK2645 reshaped the gut microbiota composition by increasing the abundance of beneficial bacterial genera such as\n                    <jats:italic>Dubosiella<\/jats:italic>\n                    ,\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    , and\n                    <jats:italic>Limosilactobacillus<\/jats:italic>\n                    , while simultaneously reducing the abundance of potentially pathogenic genera such as\n                    <jats:italic>Ileibacterium<\/jats:italic>\n                    and\n                    <jats:italic>Alistipes<\/jats:italic>\n                    . This study provides novel evidence that\n                    <jats:italic>A. muciniphila<\/jats:italic>\n                    inactivation exerts strain-specific effects on inflammation regulation. Furthermore, it indicates that pasteurized AKK2645 exerts a beneficial effect on colitis by modulating immune cell populations and microbial homeostasis.\n                  <\/jats:p>","DOI":"10.3389\/fnut.2026.1863368","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:57:16Z","timestamp":1785164236000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Strain-dependent efficacy of pasteurized Akkermansia muciniphila in alleviating colitis reveals AKK2645 restores immune and microbial homeostasis"],"prefix":"10.3389","volume":"13","author":[{"given":"Hui","family":"Li","sequence":"first","affiliation":[{"name":"College of Chemistry and Materials Science, Key Laboratory of Analytical Science and Technology of Hebei Province, and MOE Key Laboratory of Medicinal Chemistry and Molecular Diagnostics, Hebei University","place":["Baoding, Hebei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhili","family":"He","sequence":"additional","affiliation":[{"name":"Beijing Youngen Technology Co. Ltd","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minlei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Youngen Technology Co. Ltd","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases (Hepatology), Lishui Central Hospital","place":["Lishui, Zhejiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yundan","family":"Bai","sequence":"additional","affiliation":[{"name":"Health Management Medical Center, Chengdu Integrated TCM and Western Medicine Hospital","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Youngen Technology Co. Ltd","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengning","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Nutrition, The First Hospital of Hebei Medical University, Hebei Key Laboratory of Nutrition and Health","place":["Shijiazhuang, Hebei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/S2468-1253(25)00263-8","article-title":"Ulcerative colitis: moving beyond the mucosal dogma","volume":"11","author":"Eggermont","year":"2025","journal-title":"Lancet Gastroenterol Hepatol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1016\/j.dld.2016.07.012","article-title":"Nationwide prevalence and drug treatment practices of inflammatory bowel diseases in Hungary: a population-based study based on the National Health 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