{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:21:06Z","timestamp":1783513266121,"version":"3.55.0"},"reference-count":43,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Chinese yam (\n                      <jats:italic>Dioscorea opposita<\/jats:italic>\n                      Thunb.) is a widely consumed edible and medicinal crop. Its polysaccharides are promising food-derived bioactive ingredients. However, cultivar-dependent consistency remains insufficiently defined. This issue is important for functional ingredient standardization. In this study, crude polysaccharide fractions isolated from two representative cultivars, Xiao-Bai-Zui and Tie-Gun, were designated CYP-XBZ and CYP-TG and comparatively evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The molecular weight distribution, monosaccharide composition, UV\u2013visible spectra, and FT\u2013IR characteristics of the two polysaccharide fractions were compared. Their protective effects were evaluated in H9c2 cells subjected to oxygen\u2013glucose deprivation\/reoxygenation (OGD\/R) and in a rat model of myocardial ischemia\/reperfusion injury (MI\/RI). Furthermore, transcriptomics, proteomics, and Western blotting were integrated to investigate the underlying mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CYP-XBZ and CYP-TG exhibited broadly similar molecular weight distribution patterns, glucan-dominant monosaccharide compositions, and characteristic polysaccharide spectral features. However, minor differences in molecular heterogeneity and monosaccharide proportions were observed. In OGD\/R-treated H9c2 cells, both fractions significantly improved cell viability and reduced CK-MB, cTnT, and 8-OHdG levels. Multi-omics analyses revealed that the shared protective effects were mainly associated with stress and inflammation-related pathways, particularly the MyD88-associated MAPK and NF-\u03baB signaling pathways. These findings were further confirmed by Western blotting. In the rat model of MI\/RI, oral administration of both polysaccharide fractions reduced infarct size and alleviated histopathological injury. They also decreased serum biomarkers of myocardial injury and oxidative stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Polysaccharides from the two major Chinese yam cultivars showed highly comparable structural features, cardio protective effects, and regulatory mechanisms. These findings support the functional consistency of these cultivars as sources of bioactive polysaccharides and highlight their potential as food-derived functional ingredients for cardiovascular and nutrition-related health.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnut.2026.1864107","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:49:50Z","timestamp":1783511390000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative structural characterization and protective effects against myocardial ischemia\/reperfusion injury of polysaccharides from two major Chinese yam cultivars (Dioscorea opposita Thunb)"],"prefix":"10.3389","volume":"13","author":[{"given":"Guojie","family":"Xie","sequence":"first","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, School of Basic Medical Sciences and School of Pharmaceutical Sciences, Guizhou Medical University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longqin","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, School of Basic Medical Sciences and School of Pharmaceutical Sciences, Guizhou Medical University","place":["Guiyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oncology, Ningbo Municipal Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University","place":["Ningbo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, Institute of 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Dao-di Herbs, Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences","place":["Beijing, China"]},{"name":"Hebei Institute of Industrial Technology for Chinese Medicinal Materials","place":["Anguo, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Medicine, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs, Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipeng","family":"Gong","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key 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