{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:55:44Z","timestamp":1790920544870,"version":"4.1.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"\n Introduction\/objective<\/jats:title>\n \n This study aimed to evaluate whether\n Lactobacillus rhamnosus<\/jats:italic>\n and\n Lactobacillus gasseri<\/jats:italic>\n could alleviate alcohol-induced liver damage, behavioral changes, and gut microbiota dysbiosis in rats. And sought to better understand the role of Lactobacillus-mediated microbiota modulation in alcohol-induced liver damage.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Male SD rats were first subjected to 1 week of ethanol adaptation, followed by 4 weeks of feeding with a Lindros alcohol liquid diet containing 6.2% ethanol to establish the ALD model. Subsequently, the rats received 2 weeks of either\n L. rhamnosus<\/jats:italic>\n or\n L. gasseri<\/jats:italic>\n (1\u202f\u00d7\u202f10\n 9<\/jats:sup>\n CFU\/rat\/day) treatment while continuing to receive the Lindros alcohol liquid diet containing 6.2% ethanol. The control group were treated with PBS. Serum fibroblast growth factor 21 (FGF21), lipopolysaccharide (LPS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were determined, along with hepatic TNF-\u03b1 expression. Behavioral performance was assessed using the open-field test, and gut microbiota composition was analyzed by 16S rRNA gene sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Alcohol-fed rats showed significantly increases in serum FGF21, LPS, ALT, AST, and hepatic TNF-\u03b1 levels, accompanied by reduced locomotor activity and gut microbial diversity. Supplementation with either\n L. rhamnosus<\/jats:italic>\n or\n L. gasseri<\/jats:italic>\n mitigated these changes. Notably,\n Lactobacillus gasseri<\/jats:italic>\n demonstrated a more pronounced protective effect on liver injury markers.\n Lactobacillus<\/jats:italic>\n treatment partially restored microbial richness, increased beneficial genera such as\n Monoglobus<\/jats:italic>\n , and reduced alcohol-associated taxa including\n Bacteroides<\/jats:italic>\n and\n Alloprevotella<\/jats:italic>\n . Functional prediction analysis indicated significant alterations in pathways related to sulfur metabolism, purine metabolism, the sulfur cycle, and butanoate metabolism.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n \n Both\n L. rhamnosus<\/jats:italic>\n and\n L. gasseri<\/jats:italic>\n effectively attenuated alcohol-induced liver injury, inflammation, and behavioral impairments in rats. These effects are likely through modulation of gut microbiota composition and function.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Our work suggest that probiotic intervention may represent a promising therapeutic strategy for the management of alcohol-induced disorders.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fnut.2026.1887275","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:36:27Z","timestamp":1790919387000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Lactobacillus rhamnosus and Lactobacillus gasseri ameliorate alcohol-induced liver injury and gut microbiota dysbiosis in rats"],"prefix":"10.3389","volume":"13","author":[{"given":"Yi","family":"Zhang","sequence":"first","affiliation":[{"name":"Shenzhen Kangning Hospital & Shenzhen Mental Health Center","place":["Shenzhen, China"]},{"name":"Clinical College of Mental Health, Shenzhen University Health Science Center","place":["Shenzhen, China"]},{"name":"Affiliated Mental Health Center, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiting","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Medical College","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Fu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Medical College","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Medical College","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"An","sequence":"additional","affiliation":[{"name":"Research & Teaching Department, The Fourth People's Hospital of Shenzhen (Shenzhen Samii Medical Center)","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Review and Evaluation Department, The Fourth People's Hospital of Shenzhen (Shenzhen Samii Medical Center)","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Jia","sequence":"additional","affiliation":[{"name":"Shenzhen Kangning Hospital & Shenzhen Mental Health Center","place":["Shenzhen, China"]},{"name":"Clinical College of Mental Health, Shenzhen University Health Science Center","place":["Shenzhen, China"]},{"name":"Affiliated Mental Health Center, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dajun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Medical College","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"ref1","volume-title":"Global Status Report on Alcohol and Health and Treatment of Substance Use Disorders","year":"2024"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1111\/add.12876","article-title":"Prevalence of alcohol use disorders in mainland China: a systematic review","volume":"110","author":"Cheng","year":"2015","journal-title":"Addiction"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1495","DOI":"10.1111\/jnc.15284","article-title":"Microbiota-gut-brain axis as a regulator of reward processes","volume":"157","author":"Garc\u00eda-Cabrerizo","year":"2021","journal-title":"J Neurochem"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1038\/s41380-020-00970-6","article-title":"A biological framework for emotional dysregulation in alcohol misuse: from gut to 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