{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:03:38Z","timestamp":1789621418850,"version":"build-2803163510"},"reference-count":20,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Nutr."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To elucidate the anticoagulant effects of\n                      <jats:italic>Cordyceps militaris<\/jats:italic>\n                      polysaccharides and provide a scientific basis for their application in functional foods and biomedicine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Crude polysaccharide (CC-1) was extracted by water extraction and ethanol precipitation, and then purified by papain-mediated deproteinization and dialysis to obtain polysaccharide CC-2. Chemical composition was analyzed using the phenol\u2013sulfuric acid method, the meta-hydroxybiphenyl method, and a BCA protein assay kit. Molecular weight was determined by high-performance gel permeation chromatography (HPGPC). Monosaccharide composition was analyzed by PMP pre-column derivatization HPLC (PMP-HPLC). Structural features were characterized by Fourier transform infrared (FT-IR) and ultraviolet (UV) spectroscopy, and scanning electron microscopy (SEM).\n                      <jats:italic>In vitro<\/jats:italic>\n                      anticoagulant activity was evaluated by measuring activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), and fibrinogen (FIB) levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The yield of CC-2 was 2.16%. Compared with CC-1, CC-2 showed significantly higher total sugar and uronic acid contents and a decreased protein content. The molecular weight of CC-2 was 5908.612 Da. Its monosaccharide composition consisted primarily of rhamnose, glucuronic acid, glucose, galactose, and xylose in a molar ratio of 5:1:4:11:4. FT-IR indicated the presence of acetyl groups and a furanose ring conformation. SEM revealed a microstructure composed of irregular lumps and spherical particles.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , CC-2 significantly prolonged PT, APTT, and TT (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.001) by approximately 2.10, 2.46, and 2.79 fold, respectively, and significantly reduced FIB levels (\n                      <jats:italic>P<\/jats:italic>\n                      &amp;lt; 0.001). Its FIB-lowering effect was superior to that of the positive control, breviscapine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cordyceps militaris<\/jats:italic>\n                      polysaccharides exhibit significant\n                      <jats:italic>in vitro<\/jats:italic>\n                      anticoagulant activity, acting through potential multi-pathway mechanisms and pronounced fibrinogen reduction. These findings support their potential development as functional food ingredients or biomedical agents for the prevention of thrombotic cardiovascular and cerebrovascular diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fnut.2026.1915697","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:29:15Z","timestamp":1789619355000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Extraction, purification, characterization, and in vitro anticoagulant activity of polysaccharides from Cordyceps militaris"],"prefix":"10.3389","volume":"13","author":[{"given":"Cai-fang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pharmacy, Xinxiang Central Hospital","place":["Xinxiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-gan","family":"Lu","sequence":"additional","affiliation":[{"name":"Henan 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