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To provide insight into the etiology of these precancerous lesions and to explore the potential molecular mechanisms underlying their metastatic behavior, we performed a proteomic mass spectrometry analysis in a patient with synchronous EIC and STIC. Through histological and molecular identification of precancerous lesions followed by laser capture microdissection, we were able to identify over 450 proteins within the precancerous lesions and adjacent healthy tissue. The proteomic analysis of STIC and EIC showed remarkable overlap in the proteomic patterns, reflecting early neoplastic changes in proliferation, loss of polarity and attachment. Our proteomic analysis showed that both EIC and STIC, despite being regarded as premalignant lesions, have metastatic potential, which correlates with the common presentation of invasive serous gynecological malignancies at advanced stage.<\/jats:p>","DOI":"10.3389\/fonc.2020.523989","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T19:39:40Z","timestamp":1610307580000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Proteomic Analysis of Pre-Invasive Serous Lesions of the Endometrium and Fallopian Tube Reveals Their Metastatic Potential"],"prefix":"10.3389","volume":"10","author":[{"given":"Mitchell","family":"Acland","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgia","family":"Arentz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Max","family":"Mussared","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fergus","family":"Whitehead","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Klingler-Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin K.","family":"Oehler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"S96","DOI":"10.1016\/j.ijgo.2015.06.005","article-title":"Cancer of the corpus uteri","volume":"131","author":"Amant","year":"2015","journal-title":"Int J Gynaecol Obstet"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32514","article-title":"New perspectives on screening and early detection of endometrial cancer","volume":"145","author":"Costas","year":"2019","journal-title":"Int J Cancer"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-019-0177-x","article-title":"Clinical actionability of molecular targets in endometrial cancer","volume":"19","author":"Urick","year":"2019","journal-title":"Nat Rev Cancer"},{"key":"B4","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/nature12113","article-title":"Integrated genomic characterization of endometrial carcinoma","volume":"497","author":"Kandoth","year":"2013","journal-title":"Nature"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.17408.1","article-title":"Recent advances in endometrial cancer: a review of key clinical trials from 2015 to 2019","volume":"8","author":"Charo","year":"2019","journal-title":"F1000Res"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int J Cancer"},{"key":"B7","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/S1470-2045(14)71123-4","article-title":"Global burden of cancer attributable to high body-mass index in 2012: a population-based study","volume":"16","author":"Arnold","year":"2015","journal-title":"Lancet Oncol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31802ee494","article-title":"Clinicopathologic analysis of 187 high-grade endometrial carcinomas of different histologic subtypes: similar outcomes belie distinctive biologic differences","volume":"31","author":"Soslow","year":"2007","journal-title":"Am J Surg Pathol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2004.08.030","article-title":"Patients with uterine papillary serous cancers may benefit from adjuvant platinum-based chemoradiation","volume":"95","author":"Kelly","year":"2004","journal-title":"Gynecol Oncol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.05.027","article-title":"Utility of pre-operative serum CA-125 in the management of uterine papillary serous carcinoma","volume":"110","author":"Olawaiye","year":"2008","journal-title":"Gynecol Oncol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603012","article-title":"Uterine papillary serous and clear cell carcinomas predict for poorer survival compared to grade 3 endometrioid corpus cancers","volume":"94","author":"Hamilton","year":"2006","journal-title":"Br J Cancer"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1177\/106689690401200302","article-title":"Endometrial glandular dysplasia: a newly defined precursor lesion of uterine papillary serous carcinoma. 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