{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T06:00:53Z","timestamp":1783576853720,"version":"3.55.0"},"reference-count":64,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2012\/10282-5"],"award-info":[{"award-number":["2012\/10282-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9liuksen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006383","name":"Sy\u00f6p\u00e4j\u00e4rjest\u00f6t","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006383","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004012","name":"Jane ja Aatos Erkon S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004012","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To integrate mRNA and miRNA expression profiles of mucoepidermoid carcinomas (MECs) and normal salivary gland (NSGs) tissue samples and identify potential\u00a0drivers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Gene and miRNA expression arrays were performed in 35 MECs and six NSGs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found 46 differentially expressed (DE) miRNAs and 3,162 DE mRNAs. Supervised hierarchical clustering analysis of the DE transcripts revealed two clusters in both miRNA and mRNA profiles, which distinguished MEC from NSG samples. The integrative miRNA-mRNA analysis revealed a network comprising 696 negatively correlated interactions (44 miRNAs and 444 mRNAs) involving cell signaling, cell cycle, and cancer-related pathways. Increased expression levels of miR-205-5p and miR-224-5p and decreased expression levels of miR-139-3p, miR-145-3p, miR-148a-3p, miR-186-5p, miR-338-3p, miR-363-3p, and miR-4324 were significantly related to worse overall survival in MEC patients. Two overexpressed miRNAs in MEC (miR-22 and miR-205) were selected for inhibition by the CRISPR-Cas9 method. Cell viability, migration, and invasion assays were performed using an intermediate grade MEC cell line. Knockout of miR-205 reduced cell viability and enhanced <jats:italic>ZEB2<\/jats:italic> expression, while miR-22 knockout reduced cell migration and invasion and enhanced <jats:italic>ESR1<\/jats:italic> expression. Our results indicate a distinct transcriptomic profile of MEC compared to NSG, and the integrative analysis highlighted miRNA-mRNA interactions involving cancer-related pathways, including PTEN and PI3K\/AKT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The <jats:italic>in vitro<\/jats:italic> functional studies revealed that miR-22 and miR-205 deficiencies reduced the viability, migration, and invasion of the MEC cells suggesting they are potential oncogenic drivers in MEC.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2021.786150","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T07:59:13Z","timestamp":1644393553000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["miR-22 and miR-205 Drive Tumor Aggressiveness of Mucoepidermoid Carcinomas of Salivary Glands"],"prefix":"10.3389","volume":"11","author":[{"given":"Erika","family":"Naakka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Camargo","family":"Barros-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shady","family":"Adnan-Awad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Al-Samadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Albuquerque","family":"Marchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hellen","family":"Kuasne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Korelin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilida","family":"Suleymanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy Louise","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristovam","family":"Scapulatempo-Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Vanessa","family":"Louren\u00e7o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rog\u00e9rio Moraes","family":"Castilho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Paulo","family":"Kowalski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"M\u00e4kitie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera Cavalcanti","family":"Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilmo","family":"Leivo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Regina","family":"Rogatto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabricio","family":"Passador-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"B1","article-title":"Mucoepidemoid Carcinoma","volume-title":"WHO Classification of Head and Neck Tumors.","author":"Brandwein-Gensler","year":"2017"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31815a6b0b","article-title":"Management and Outcome of Patients With Mucoepidermoid Carcinoma of Major Salivary Gland Origin: A Single Institution\u2019s 30-Year Experience","volume":"118","author":"Aro","year":"2008","journal-title":"Laryngoscope"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23825","article-title":"Treatment and Survival Outcomes Based on Histologic Grading in Patients With Head and Neck Mucoepidermoid Carcinoma","volume":"113","author":"Nance","year":"2008","journal-title":"Cancer"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19920415)69:8<2021::AID-CNCR2820690803>3.0.CO;2-7","article-title":"Mucoepidermoid Carcinoma of Intraoral Salivary Glands Evaluation and Application of Grading Criteria in 143 Cases","volume":"69","author":"Auclair","year":"1992","journal-title":"Cancer"},{"key":"B5","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/s12105-009-0102-9","article-title":"An Update on Grading of Salivary Gland Carcinomas","volume":"3","author":"Seethala","year":"2009","journal-title":"Head Neck Pathol"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200107000-00001","article-title":"Mucoepidermoid Carcinoma: A Clinicopathologic Study of 80 Patients With Special Reference to Histological Grading","volume":"25","author":"Brandwein","year":"2001","journal-title":"Am J Surg Pathol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1002\/HED.23467","article-title":"Patterns of Nodal Involvement for Clinically N0 Saliavry Gland Carcinoma: Refining the Role of Elective Neck Irradiation","volume":"36","author":"Lau","year":"2014","journal-title":"Head Neck"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1002\/lary.25588","article-title":"What is the Role of Elective Neck Dissection in Low-, Intermediate-, and High-Grade Mucoepidermoid Carcinoma","volume":"126","author":"Moss","year":"2016","journal-title":"Laryngoscope"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2013.05.024","article-title":"Head and Neck Salivary Gland Carcinomas - Elective Neck Dissection, Yes or No","volume":"72","author":"Nobis","year":"2014","journal-title":"J Oral Maxillofac Surg"},{"key":"B10","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.humpath.2020.02.001","article-title":"Pathologic Grading of Mucoepidermoid Carcinomas of the Salivary Gland and Its Effect on Clinicopathologic Follow-Up: An Institutional Experience","volume":"98","author":"Shafique","year":"2020","journal-title":"Hum Pathol"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1038\/ng1083","article-title":"T(11;19)(Q21;P13) Translocation in Mucoepidermoid Carcinoma Creates a Novel Fusion Product That Disrupts a Notch Signaling Pathway","volume":"33","author":"Tonon","year":"2003","journal-title":"Nat Genet"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20458","article-title":"CRTC1\/MAML2 Fusion Transcript in High Grade Mucoepidermoid Carcinomas of Salivary and Thyroid Glands and Warthin\u2019s Tumors: Implications for Histogenesis and Biologic Behavior","volume":"46","author":"Tirado","year":"2007","journal-title":"Genes Chromosomes Cancer"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.154","article-title":"Genomic Profiles and CRTC1-MAML2 Fusion Distinguish Different Subtypes of Mucoepidermoid Carcinoma","volume":"26","author":"Jee","year":"2013","journal-title":"Mod Pathol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2015.3270","article-title":"Role of CRTC1\/MAML2 Translocation in the Prognosis and Clinical Outcomes of Mucoepidermoid Carcinoma","volume":"142","author":"Saade","year":"2016","journal-title":"JAMA Otolaryngol Head Neck Surg"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.01.003","article-title":"Diagnostic and Prognostic Utility of Mastermind-Like 2 (MAML2) Gene Rearrangement Detection by Fluorescent In Situ Hybridization (FISH) in Mucoepidermoid Carcinoma of the Salivary Glands","volume":"121","author":"Luk","year":"2016","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3892\/or_00000438","article-title":"Chromosomal Imbalances, 11q21 Rearrangement and MECT1-MAML2 Fusion Transcript in Mucoepidermoid Carcinomas of the Salivary Gland","volume":"22","author":"Verdorfer","year":"2009","journal-title":"Oncol Rep"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181de3021","article-title":"A Reappraisal of the MECT1\/MAML2 Translocation in Salivary Mucoepidermoid Carcinomas","volume":"34","author":"Seethala","year":"2010","journal-title":"Am J Surg Pathol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20306","article-title":"Molecular Classification of Mucoepidermoid Carcinomas-Prognostic Significance of the MECT1-MAML2 Fusion Oncogene","volume":"45","author":"Behboudi","year":"2006","journal-title":"Genes Chromosomes Cancer"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2376","article-title":"MECT1-MAML2 Fusion Transcript Defines a Favorable Subset of Mucoepidermoid Carcinoma","volume":"12","author":"Okabe","year":"2006","journal-title":"Clin Cancer Res"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14632","article-title":"Prognostic Impact of CRTC1\/3-MAML2 Fusions in Salivary Gland Mucoepidermoid Carcinoma: A Multiinstitutional Retrospective Study","volume":"111","author":"Okumura","year":"2020","journal-title":"Cancer Sci"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000676","article-title":"MAML2 Status in Mucoepidermoid Carcinoma Can No Longer Be Considered a Prognostic Marker","volume":"40","author":"Seethala","year":"2016","journal-title":"Am J Surg Pathol"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2004.04.016","article-title":"Characterization of Gene Expression in Major Types of Salivary Gland Carcinomas With Epithelial Differentiation","volume":"156","author":"Leivo","year":"2005","journal-title":"Cancer Genet Cytogenet"},{"key":"B23","article-title":"Identification of Differentially Expressed Genes in Human Salivary Gland Tumors by DNA Microarrays","volume":"1","author":"Francioso","year":"2002","journal-title":"Mol Cancer Ther"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-016-2049-z","article-title":"Altered Expression of Apoptosis-Regulating miRNAs in Salivary Gland Tumors Suggests Their Involvement in Salivary Gland Tumorigenesis","volume":"470","author":"Flores B de CT de","year":"2017","journal-title":"Virchows Arch"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2017.10.012","article-title":"MicroRNAs Expression Pattern Related to Mast Cell Activation and Angiogenesis in Paraffin-Embedded Salivary Gland Tumors","volume":"213","author":"Santos","year":"2017","journal-title":"Pathol Res Pract"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12800","article-title":"miRNA Expression Profile of Mucoepidermoid Carcinoma","volume":"24","author":"Binmadi","year":"2018","journal-title":"Oral Dis"},{"key":"B27","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.oraloncology.2019.04.018","article-title":"Integrated miRNA and mRNA Expression Analysis Uncovers Drug Targets in Laryngeal Squamous Cell Carcinoma Patients","volume":"93","author":"Lapa","year":"2019","journal-title":"Oral Oncol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz896","article-title":"MiRTarBase 2020: Updates to the Experimentally Validated microRNA-Target Interaction Database","volume":"48","author":"Huang","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.08.004","article-title":"Characterization of Tumorigenic Cell Lines From the Recurrence and Lymph Node Metastasis of a Human Salivary Mucoepidermoid Carcinoma","volume":"49","author":"Warner","year":"2013","journal-title":"Oral Oncol"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.143","article-title":"Genome Engineering Using CRISPR-Cas9 System","volume":"8","author":"Ran","year":"2013","journal-title":"Nat Protoc"},{"key":"B31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12885-015-1944-z","article-title":"A Novel Human Leiomyoma Tissue Derived Matrix for Cell Culture Studies","volume":"15","author":"Salo","year":"2015","journal-title":"BMC Cancer"},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3791\/59567","article-title":"Fully Human Tumor-Based Matrix in Three-Dimensional Spheroid Invasion Assay","volume":"2019","author":"Naakka","year":"2019","journal-title":"J Vis Exp"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019.Fiji","article-title":"Fiji - An Open Platform for Biological Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01875-08","article-title":"miR-22 Inhibits Estrogen Signaling by Directly Targeting the Estrogen Receptor \u03b1 mRNA","volume":"29","author":"Pandey","year":"2009","journal-title":"Mol Cell Biol"},{"key":"B35","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1479-5876-9-30","article-title":"MiRNA-205 Modulates Cellular Invasion and Migration via Regulating Zinc Finger E-Box Binding Homeobox 2 Expression in Esophageal Squamous Cell Carcinoma Cells","volume":"9","author":"Matsushima","year":"2011","journal-title":"J Transl Med"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2019.10126","article-title":"Zinc Finger E\u2212box Binding Homeobox 2 Functions as an Oncogene in Human Laryngeal Squamous Cell Carcinoma","volume":"6","author":"Li","year":"2019","journal-title":"Mol Med Rep"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001252","article-title":"Mucoepidermoid Carcinoma: A Comparison of Histologic Grading Systems and Relationship to MAML2 Rearrangement and Prognosis","volume":"43","author":"Cipriani","year":"2019","journal-title":"Am J Surg Pathol"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2018.12.003","article-title":"The Reliability of MAML2 Gene Rearrangement in Discriminating Between Histologically Similar Glandular Odontogenic Cysts and Intraosseous Mucoepidermoid Carcinomas","volume":"127","author":"Reddy","year":"2019","journal-title":"Oral Surg Oral Med Oral Pathol Oral Radiol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-014-0534-8","article-title":"Glandular Odontogenic Cysts (GOCs) Lack MAML2 Rearrangements: A Finding to Discredit the Putative Nature of GOC as a Precursor to Central Mucoepidermoid Carcinoma","volume":"8","author":"Bishop","year":"2014","journal-title":"Head Neck Pathol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1111\/his.12526","article-title":"Fluorescence in-Situ Hybridization Identifies Mastermind-Like 2 (MAML2) Rearrangement in Odontogenic Cysts With Mucous Prosoplasia: A Pilot Study","volume":"66","author":"Argyris","year":"2015","journal-title":"Histopathology"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2003.08.013","article-title":"TORCs: Transducers of Regulated CREB Activity","volume":"12","author":"Conkright","year":"2003","journal-title":"Mol Cell"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1125","article-title":"Mect1-Maml2 Fusion Oncogene Linked to the Aberrant Activation of Cyclic AMP\/CREB Regulated Genes","volume":"65","author":"Coxon","year":"2005","journal-title":"Cancer Res"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2003.09.007","article-title":"Altered Notch Signaling Resulting From Expression of a WAMTP1-MAML2 Gene Fusion in Mucoepidermoid Carcinomas and Benign Warthin\u2019s Tumors","volume":"292","author":"Enlund","year":"2004","journal-title":"Exp Cell Res"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600719","article-title":"Transforming Activity of MECT1-MAML2 Fusion Oncoprotein Is Mediated by Constitutive CREB Activation","volume":"24","author":"Wu","year":"2005","journal-title":"EMBO J"},{"key":"B45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12885-015-1827-3","article-title":"Gene Expression Profiling Analysis of CRTC1-MAML2 Fusion Oncogene-Induced Transcriptional Program in Human Mucoepidermoid Carcinoma Cells","volume":"15","author":"Chen","year":"2015","journal-title":"BMC Cancer"},{"key":"B46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1172\/jci.insight.139497","article-title":"The CRTC1-MAML2 Fusion Is the Major Oncogenic Driver in Mucoepidermoid Carcinoma","volume":"6","author":"Chen","year":"2021","journal-title":"JCI Insight"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0210968","article-title":"A microRNA Signature for the Differential Diagnosis of Salivary Gland Tumors","volume":"14","author":"Denaro","year":"2019","journal-title":"PLoS One"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyx073","article-title":"miR-582-5p Inhibits Invasion and Migration of Salivary Adenoid Cystic Carcinoma Cells by Targeting FOXC1","volume":"47","author":"Wang","year":"2017","journal-title":"Jpn J Clin Oncol"},{"key":"B49","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1016\/j.tcb.2009.10.001","article-title":"Polycomb Group Complexes - Many Combinations, Many Functions","volume":"19","author":"Kerppola","year":"2009","journal-title":"Trends Cell Biol"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03184.x","article-title":"Deregulated Expression of P16ink4a and P53 Pathway Members in Benign and Malignant Myoepithelial Tumours of the Salivary Glands","volume":"53","author":"V\u00e9kony","year":"2008","journal-title":"Histopathology"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2007.054262","article-title":"High Expression of Polycomb Group Protein EZH2 Predicts Poor Survival in Salivary Gland Adenoid Cystic Carcinoma","volume":"61","author":"V\u00e9kony","year":"2008","journal-title":"J Clin Pathol"},{"key":"B52","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13000-015-0392-z","article-title":"EZH2 Is a Sensitive Marker of Malignancy in Salivary Gland Tumors","volume":"10","author":"Haj\u00f3si-Kalcakosz","year":"2015","journal-title":"Diagn Pathol"},{"key":"B53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13058-019-1098-z","article-title":"A Subgroup of microRNAs Defines PTEN-Deficient, Triple-Negative Breast Cancer Patients With Poorest Prognosis and Alterations in RB1, MYC, and Wnt Signaling","volume":"21","author":"Wang","year":"2019","journal-title":"Breast Cancer Res"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw689","article-title":"Comprehensive Genomic Profiling of Salivary Mucoepidermoid Carcinomas Reveals Frequent BAP1, PIK3CA, and Other Actionable Genomic Alterations","volume":"28","author":"Wang","year":"2017","journal-title":"Ann Oncol"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14578","article-title":"PLCA8 Suppresses Breast Cancer Apoptosis by Activating the PI3k\/AKT\/NF-\u03bab Pathway","volume":"23","author":"Mao","year":"2019","journal-title":"J Cell Mol Med"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.21873\/anticanreS.11715","article-title":"MicroRNA Profiling and Target Genes Related to Metastasis of Salivary Adenoid Cystic Carcinoma","volume":"37","author":"Feng","year":"2017","journal-title":"Anticancer Res"},{"key":"B57","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12916-015-0350-3","article-title":"Accuracy of microRNAs as Markers for the Detection of Neck Lymph Node Metastases in Patients With Head and Neck Squamous Cell Carcinoma","volume":"13","author":"de Carvalho","year":"2015","journal-title":"BMC Med"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2019.05.058","article-title":"miR-205-5p Regulates Epithelial-Mesenchymal Transition by Targeting PTEN via PI3K\/AKT Signaling Pathway in Cisplatin-Resistant Nasopharyngeal Carcinoma Cells","volume":"710","author":"Zhang","year":"2019","journal-title":"Gene"},{"key":"B59","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0142373","article-title":"Dual Action of miR-125b as a Tumor Suppressor and OncomiR-22 Promotes Prostate Cancer Tumorigenesis","volume":"10","author":"Budd","year":"2015","journal-title":"PLoS One"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-2475","article-title":"Tumor Suppressor miR-22 Determines P53-Dependent Cellular Fate Through Post-Transcriptional Regulation of P21","volume":"71","author":"Tsuchiya","year":"2011","journal-title":"Cancer Res"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010100","article-title":"miR-22 Represses Cancer Progression by Inducing Cellular Senescence","volume":"193","author":"Xu","year":"2011","journal-title":"J Cell Biol"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.4161\/cc.27027","article-title":"MiR-22 in Tumorigenesis","volume":"13","author":"Song","year":"2014","journal-title":"Cell Cycle"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.18389","article-title":"C-Fos-Dependent miR-22 Targets MDC1 and Regulates DNA Repair in Terminally Differentiated Cells","volume":"8","author":"Lee","year":"2017","journal-title":"Oncotarget"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.4161\/cc.7.20.6851","article-title":"MicroRNAs as Regulators of Epithelial-Mesenchymal Transition","volume":"7","author":"Gregory","year":"2008","journal-title":"Cell Cycle"}],"container-title":["Frontiers in Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2021.786150\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T07:59:22Z","timestamp":1644393562000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2021.786150\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,9]]},"references-count":64,"alternative-id":["10.3389\/fonc.2021.786150"],"URL":"https:\/\/doi.org\/10.3389\/fonc.2021.786150","relation":{},"ISSN":["2234-943X"],"issn-type":[{"value":"2234-943X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,9]]},"article-number":"786150"}}