{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:27:43Z","timestamp":1788222463233,"version":"build-2803163510"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T00:00:00Z","timestamp":1651795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011851","name":"Kom op tegen Kanker","doi-asserted-by":"publisher","award":["KOTK UA\/2016\/10710\/1, 'Emmanuel van der Schueren'"],"award-info":[{"award-number":["KOTK UA\/2016\/10710\/1, 'Emmanuel van der Schueren'"]}],"id":[{"id":"10.13039\/501100011851","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007660","name":"Universiteit Antwerpen","doi-asserted-by":"publisher","award":["BOF-KP 36051"],"award-info":[{"award-number":["BOF-KP 36051"]}],"id":[{"id":"10.13039\/501100007660","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Malignant pleural mesothelioma (MPM) is a lethal cancer for which early-stage diagnosis remains a major challenge. Volatile organic compounds (VOCs) in breath proved to be potential biomarkers for MPM diagnosis, but translational studies are needed to elucidate which VOCs originate from the tumor itself and thus are specifically related to MPM cell metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An <jats:italic>in vitro<\/jats:italic> model was set-up to characterize the headspace VOC profiles of six MPM and two lung cancer cell lines using thermal desorption-gas chromatography-mass spectrometry. A comparative analysis was carried out to identify VOCs that could discriminate between MPM and lung cancer, as well as between the histological subtypes within MPM (epithelioid, sarcomatoid and biphasic).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>VOC profiles were identified capable of distinguishing MPM (subtypes) and lung cancer cells with high accuracy. Alkanes, aldehydes, ketones and alcohols represented many of the discriminating VOCs. Discrepancies with clinical findings were observed, supporting the need for studies examining breath and tumor cells of the same patients and studying metabolization and kinetics of <jats:italic>in vitro<\/jats:italic> discovered VOCs in a clinical setting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While the relationship between <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> VOCs is yet to be established, both could complement each other in generating a clinically useful breath model for MPM.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2022.851785","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T06:55:22Z","timestamp":1651820122000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Headspace Volatile Organic Compound Profiling of Pleural Mesothelioma and Lung Cancer Cell Lines as Translational Bridge for Breath Research"],"prefix":"10.3389","volume":"12","author":[{"given":"Eline","family":"Janssens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zo\u00eb","family":"Mol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lore","family":"Vandermeersch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Lagniau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim Y.","family":"Vermaelen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan P.","family":"van Meerbeeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Walgraeve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elly","family":"Marcq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Lamote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3238\/arztebl.2013.0319","article-title":"Malignant Pleural Mesothelioma: Incidence, Etiology, Diagnosis, Treatment, and Occupational Health","volume":"110","author":"Neumann","year":"2013","journal-title":"Dtsch Arztebl Int"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.01519","article-title":"Malignant Pleural Mesothelioma: State-Of-the-Art on Current Therapies and\u00a0Promises for the Future","volume":"9","author":"Nicolini","year":"2020","journal-title":"Front Oncol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1164\/RCCM.201901-0096OC","article-title":"Pleural Plaques and the Risk of Lung Cancer in Asbestos-Exposed Subjects","volume":"201","author":"Brims","year":"2019","journal-title":"Am J Respir Crit Care Med"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2018.06.011","article-title":"Scientific Advances and New Frontiers in Mesothelioma Therapeutics","volume":"13","author":"Mutti","year":"2018","journal-title":"J Thorac Oncol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2072-1439.2013.11.28","article-title":"Guidelines for the Diagnosis and Treatment of Malignant Pleural Mesothelioma","volume":"5","author":"van Zandwijk","year":"2013","journal-title":"J Thorac Dis"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3390\/JCM10112434","article-title":"When the Diagnosis of Mesothelioma Challenges Textbooks and Guidelines","volume":"10","author":"Rossi","year":"2021","journal-title":"J Clin Med"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1912719","article-title":"Perspectives on the Treatment of Malignant Pleural Mesothelioma","volume":"385","author":"Janes","year":"2021","journal-title":"N Engl J Med"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2017.05.06","article-title":"Diagnostic and Prognostic Biomarkers for Malignant Mesothelioma: An Update","volume":"6","author":"Chen","year":"2017","journal-title":"Transl Lung Cancer Res"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2017.10.09","article-title":"Clinical Diagnosis of\u00a0Malignant Pleural Mesothelioma","volume":"10","author":"Bianco","year":"2018","journal-title":"J Thorac Dis"},{"key":"B10","doi-asserted-by":"publisher","first-page":"210057","DOI":"10.1183\/16000617.0057-2021","article-title":"Clinical Utility of Diagnostic Biomarkers in Malignant Pleural Mesothelioma: A Systematic Review and Meta-Analysis","volume":"30","author":"Schillebeeckx","year":"2021","journal-title":"Eur Respir Rev"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.21037\/tlcr.2018.04.09","article-title":"Breath Analysis as a Diagnostic and Screening Tool for Malignant Pleural Mesothelioma: A Systematic Review","volume":"7","author":"Brusselmans","year":"2018","journal-title":"Transl Lung Cancer Res"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11060831","article-title":"Breath Analysis: A Systematic Review of Volatile Organic Compounds (VOCs) in Diagnostic and Therapeutic Management of Pleural Mesothelioma","volume":"11","author":"Catino","year":"2019","journal-title":"Cancers (Basel)"},{"key":"B13","doi-asserted-by":"publisher","first-page":"1700919","DOI":"10.1183\/13993003.00919-2017","article-title":"Exhaled Breath to Screen for Malignant Pleural Mesothelioma: A Validation Study","volume":"50","author":"Lamote","year":"2017","journal-title":"Eur Respir J"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21335","article-title":"Breath Analysis by Gas Chromatography-Mass Spectrometry and Electronic Nose to Screen for Pleural Mesothelioma: A Cross-Sectional Case-Control Study","volume":"8","author":"Lamote","year":"2017","journal-title":"Oncotarget"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1088\/1752-7155\/10\/4\/046001","article-title":"Detection of Malignant Pleural Mesothelioma in Exhaled Breath by Multicapillary Column\/Ion Mobility Spectrometry (MCC\/IMS)","volume":"10","author":"Lamote","year":"2016","journal-title":"J Breath Res"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.2174\/0929867323666160510122913","article-title":"Troppmair J","volume":"23","author":"Filipiak","year":"2016","journal-title":"A Curr Med Chem"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2020.103037","article-title":"Volatile Organic Compounds in Human Matrices as Lung Cancer Biomarkers: A Systematic Review","volume":"153","author":"Janssens","year":"2020","journal-title":"Crit Rev Oncol Hematol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2007.02.005","article-title":"In Vitro Discrimination of Tumor Cell Lines With an Electronic Nose","volume":"137","author":"Gendron","year":"2007","journal-title":"Otolaryngol Head Neck Surg"},{"key":"B19","doi-asserted-by":"publisher","first-page":"046011","DOI":"10.1088\/1752-7163\/abaaff","article-title":"Headspace Analysis of Mesothelioma Cell Lines Differentiates Biphasic and Epithelioid Sub-Types","volume":"14","author":"Little","year":"2020","journal-title":"J Breath Res"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.atmosenv.2011.07.007","article-title":"Diffusive Sampling of 25 Volatile Organic Compounds in Indoor Air: Uptake Rate Determination and Application in Flemish Homes for the Elderly","volume":"45","author":"Walgraeve","year":"2011","journal-title":"Atmos Environ"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.7b02035","article-title":"Detection of Lung Cancer: Concomitant Volatile Organic Compounds and Metabolomic Profiling of Six Cancer Cell Lines of Different Histological Origins","volume":"3","author":"Jia","year":"2018","journal-title":"ACS Omega"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3390\/metabo9030052","article-title":"Critical Review of Volatile Organic Compound Analysis in Breath and In Vitro Cell Culture for Detection of Lung Cancer","volume":"9","author":"Jia","year":"2019","journal-title":"Metabolites"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2013.01.008","article-title":"Volatile Fingerprints of Cancer Specific Genetic Mutations","volume":"9","author":"Peled","year":"2013","journal-title":"Nanomedicine"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1093\/biomethods\/bpz014","article-title":"Digging Deeper Into Volatile Organic Compounds Associated With Cancer","volume":"4","author":"Janfaza","year":"2019","journal-title":"Biol Methods Protoc"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5938","article-title":"Detection of Cancer Through Exhaled Breath: A Systematic Review","volume":"6","author":"Krilaviciute","year":"2015","journal-title":"Oncotarget"},{"key":"B26","doi-asserted-by":"publisher","first-page":"190002","DOI":"10.1183\/16000617.0002-2019","article-title":"Breath Analysis of Cancer in the Present and the Future","volume":"28","author":"Amor","year":"2019","journal-title":"Eur Respir Rev"},{"key":"B27","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1186\/s12885-018-4235-7","article-title":"A Study on Volatile Organic Compounds Emitted by In-Vitro Lung Cancer Cultured Cells Using Gas Sensor Array and SPME-GCMS","volume":"18","author":"Thriumani","year":"2018","journal-title":"BMC Cancer"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)07552-7","article-title":"Volatile Organic Compounds in Breath as Markers of Lung Cancer: A Cross-Sectional Study","volume":"353","author":"Phillips","year":"1999","journal-title":"Lancet"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1021\/cr300174a","article-title":"Volatile Organic Compounds of Lung Cancer and Possible Biochemical Pathways","volume":"112","author":"Hakim","year":"2012","journal-title":"Chem Rev"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17031110","article-title":"Exhaled Breath Analysis in Diagnosis of Malignant Pleural Mesothelioma: Systematic Review","volume":"17","author":"T\u00f6reyin","year":"2020","journal-title":"Int J Environ Res Public Health"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2015.07.005","article-title":"Breath Carbonyl Compounds as Biomarkers of Lung Cancer","volume":"90","author":"Li","year":"2015","journal-title":"Lung Cancer"},{"key":"B32","doi-asserted-by":"publisher","first-page":"047105","DOI":"10.1088\/1752-7163\/aa83bb","article-title":"Strategies for the Identification of Disease-Related Patterns of Volatile Organic Compounds: Prediction of Paratuberculosis in an Animal Model Using Random Forests","volume":"11","author":"Kasbohm","year":"2017","journal-title":"J Breath Res"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4238-y","article-title":"Chemical Characterization of Exhaled Breath to Differentiate Between Patients With Malignant Pleural Mesothelioma From Subjects With Similar Professional Asbestos Exposure","volume":"398","author":"de Gennaro","year":"2010","journal-title":"Anal Bioanal Chem"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12051262","article-title":"Breath Analysis for Early Detection of Malignant Pleural Mesothelioma: Volatile Organic Compounds (VOCs) Determination and Possible Biochemical Pathways","volume":"12","author":"Di Gilio","year":"2020","journal-title":"Cancers (Basel)"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1088\/1752-7155\/8\/2\/027109","article-title":"Cell Culture Metabolomics in the Diagnosis of Lung Cancer\u2014the Influence of Cell Culture Conditions","volume":"8","author":"Kalluri","year":"2014","journal-title":"J Breath Res"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2015\/981458","article-title":"Current Challenges in Volatile Organic Compounds Analysis as Potential Biomarkers of Cancer","volume":"2015","author":"Schmidt","year":"2015","journal-title":"J Biomarkers"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1088\/1752-7155\/8\/1\/014001","article-title":"A Review of the Volatiles From the Healthy Human Body","volume":"8","author":"De Lacy Costello","year":"2014","journal-title":"J Breath Res"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00141.2020","article-title":"Translational Physiology: Normal Mesothelial Cell Lines Newly Derived From Human Pleural Biopsy Explants","volume":"319","author":"Pruett","year":"2020","journal-title":"Am J Physiol Lung Cell Mol Physiol"}],"container-title":["Frontiers in Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2022.851785\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T06:55:26Z","timestamp":1651820126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2022.851785\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,6]]},"references-count":38,"alternative-id":["10.3389\/fonc.2022.851785"],"URL":"https:\/\/doi.org\/10.3389\/fonc.2022.851785","relation":{},"ISSN":["2234-943X"],"issn-type":[{"value":"2234-943X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,6]]},"article-number":"851785"}}