{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:45:26Z","timestamp":1793036726229,"version":"4.5.0"},"reference-count":108,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:p>\n                    Human papillomavirus (HPV) is responsible for most cervical cancers and some head and neck cancers, including oropharyngeal squamous cell carcinoma and sinonasal carcinoma. Cervical cancer is commonly diagnosed by liquid-based cytology, followed by HPV testing using commercially available DNA polymerase chain reaction (PCR), p16 immunohistochemistry (IHC), or DNA\/RNA\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization. HPV in head and neck cancers is commonly diagnosed by p16 IHC or by RT-qPCR of HPV-16 E6 and E7 oncoproteins. Droplet digital PCR has been reported as an ultrasensitive and highly precise method of nucleic acid quantification for biomarker analysis and has been used to detect oncogenic HPV in oropharyngeal and cervical cancers.\n                  <\/jats:p>","DOI":"10.3389\/fonc.2022.864820","type":"journal-article","created":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T05:04:52Z","timestamp":1651035892000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":119,"title":["Molecular Detection Methods in HPV-Related Cancers"],"prefix":"10.3389","volume":"12","author":[{"given":"Jordana","family":"Williams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morris","family":"Kostiuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent L.","family":"Biron","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2020.552028","article-title":"Epidemiology and Burden of Human Papillomavirus and Related Diseases, Molecular Pathogenesis, and Vaccine Evaluation","volume":"8","author":"Kombe","year":"2021","journal-title":"Front Public Health"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/s2214-109x(16)30143-7","article-title":"Global Burden of Cancers Attributable to Infections in 2012: A Synthetic Analysis","volume":"4","author":"Plummer","year":"2016","journal-title":"Lancet Global Health"},{"key":"B3","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.bpobgyn.2017.08.006","article-title":"Epidemiology and Burden of HPV-Related Disease","volume":"47","author":"Serrano","year":"2018","journal-title":"Best Pract Res Clin Obstet Gynaecol"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30716","article-title":"Worldwide Burden of Cancer Attributable to HPV by Site, Country and HPV Type","volume":"141","author":"de Martel","year":"2017","journal-title":"Int J Cancer"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1007\/s40136-016-0134-0","article-title":"Malignant Primary Neoplasms of the Nasal Cavity and Paranasal Sinus","volume":"4","author":"Kilic","year":"2016","journal-title":"Curr Otorhinolaryngol Rep"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2016.06.006","article-title":"Burden of HPV-Positive Oropharynx Cancers Among Ever and Never Smokers in the U.S. Population","volume":"60","author":"Chaturvedi","year":"2016","journal-title":"Oral Oncol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1186\/1758-3284-2-15","article-title":"Meta-Analysis of the Impact of Human Papillomavirus (HPV) on Cancer Risk and Overall Survival in Head and Neck Squamous Cell Carcinomas (HNSCC)","volume":"2","author":"Dayyani","year":"2010","journal-title":"Head Neck Oncol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v5.i4.781","article-title":"Clinical and Scientific Impact of Human Papillomavirus on Head and Neck Cancer","volume":"5","author":"Friedman","year":"2014","journal-title":"World J Clin Oncol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2020-206686","article-title":"HPV-Related Squamous Cell Carcinoma of Oropharynx: A Review","volume":"73","author":"Rahimi","year":"2020","journal-title":"J Clin Pathol"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1111\/his.14212","article-title":"P16 Immunoexpression in Sinonasal and Nasopharyngeal Adenoid Cystic Carcinomas: A Potential Pitfall in Ruling Out HPV\u2010related Multiphenotypic Sinonasal Carcinoma","volume":"77","author":"Antony","year":"2020","journal-title":"Histopathology"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2018-0027-rs","article-title":"Human Papillomavirus\u2013Related Carcinoma With Adenoid Cystic\u2013like Features of the Sinonasal Tract (Also Known as Human Papillomavirus\u2013Related Multiphenotypic Sinonasal Carcinoma)","volume":"143","author":"Chen","year":"2019","journal-title":"Arch Pathol Lab Med"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2007.10.001","article-title":"Human Papillomavirus Detection and Utility of Testing","volume":"29","author":"Burd","year":"2007","journal-title":"Clin Microbiol Newsl"},{"key":"B13","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1128\/cmr.00013-15","article-title":"Human Papillomavirus Laboratory Testing: The Changing Paradigm","volume":"29","author":"Burd","year":"2016","journal-title":"Clin Microbiol Rev"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.13112","article-title":"A Systematic Review of Self\u2010Sampling for HPV Testing in Africa","volume":"149","author":"Nodjikouambaye","year":"2020","journal-title":"Int J Gynecol Obstet"},{"key":"B15","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.ypmed.2017.05.021","article-title":"High-Risk Human Papillomavirus Detection in Self-Sampling Compared to Physician-Taken Smear in a Responder Population of the Dutch Cervical Screening: Results of the VERA Study","volume":"101","author":"Ketelaars","year":"2017","journal-title":"Prev Med"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1007\/s11606-018-4404-z","article-title":"HPV Self-Sampling for Cervical Cancer Screening Among Ethnic Minority Women in South Florida: A Randomized Trial","volume":"33","author":"Carrasquillo","year":"2018","journal-title":"J Gen Intern Med"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.1.81","article-title":"HPV DNA Testing of Self-Collected Vaginal Samples Compared With Cytologic Screening to Detect Cervical Cancer","volume":"283","author":"Wright","year":"2000","journal-title":"Jama"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoto.2013.5460","article-title":"High-Risk Human Papillomavirus Detection in Oropharyngeal, Nasopharyngeal, and Oral Cavity Cancers: Comparison of Multiple Methods","volume":"139","author":"Walline","year":"2013","journal-title":"JAMA Otolaryngol Head Neck Surg"},{"key":"B20","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1007\/s12105-012-0370-7","article-title":"HPV Specific Testing: A Requirement for Oropharyngeal Squamous Cell Carcinoma Patients","volume":"6","author":"Robinson","year":"2012","journal-title":"Head Neck Pathol"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2019.10.008","article-title":"Human Papilloma Virus Related Squamous Cell Carcinomas of the Head and Neck: Diagnosis, Clinical Implications and Detection of HPV","volume":"52","author":"Paver","year":"2020","journal-title":"Pathology"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2017-0286-cp","article-title":"Human Papillomavirus Testing in Head and Neck Carcinomas: Guideline From the College of American Pathologists","volume":"142","author":"Lewis","year":"2017","journal-title":"Arch Pathol Lab Med"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2633","article-title":"Absolute Quantification by Droplet Digital PCR Versus Analog Real-Time PCR","volume":"10","author":"Hindson","year":"2013","journal-title":"Nat Methods"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1021\/ac202028g","article-title":"High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number","volume":"83","author":"Hindson","year":"2011","journal-title":"Anal Chem"},{"key":"B25","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/s40463-016-0177-8","article-title":"Ultrasensitive Detection of Oncogenic Human Papillomavirus in Oropharyngeal Tissue Swabs","volume":"46","author":"Isaac","year":"2017","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.29976","article-title":"Detection of Human Papillomavirus Type 16 in Oropharyngeal Squamous Cell Carcinoma Using Droplet Digital Polymerase Chain Reaction","volume":"122","author":"Biron","year":"2016","journal-title":"Cancer"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.591452","article-title":"Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method","volume":"11","author":"Rotondo","year":"2020","journal-title":"Front Microbiol"},{"key":"B28","doi-asserted-by":"publisher","first-page":"ftz048","DOI":"10.1093\/femspd\/ftz048","article-title":"Association of Human Papillomavirus Infection and Inflammation in Cervical Cancer","volume":"77","author":"Hemmat","year":"2019","journal-title":"Pathog Dis"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpobgyn.2005.02.002","article-title":"Role of Human Papillomavirus in the Carcinogenesis of Squamous Cell Carcinoma and Adenocarcinoma of the Cervix","volume":"19","author":"Tjalmar","year":"2005","journal-title":"Best Pract Res Cl Ob"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.30588","article-title":"Human Papillomavirus in Cervical Cancer and Oropharyngeal Cancer: One Cause, Two Diseases","volume":"123","author":"Berman","year":"2017","journal-title":"Cancer"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32804","article-title":"HPV Circulating Tumoral DNA Quantification by Droplet\u2010Based Digital PCR: A Promising Predictive and Prognostic Biomarker for HPV\u2010associated Oropharyngeal Cancers","volume":"147","author":"Veyer","year":"2020","journal-title":"Int J Cancer"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.2310\/7070.2010.100093","article-title":"Oropharyngeal Squamous Cell Carcinoma and Human Papillomavirus-Associated Cancers in Women: Epidemiologic Evaluation of Association","author":"Biron","year":"2011","journal-title":"J Otolaryngol Head Neck Surg"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.coms.2020.04.002","article-title":"Global Burden of Head and Neck Cancer","volume":"32","author":"Rahman","year":"2020","journal-title":"Oral Maxillofac Surg Clin North Am"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0b013e3182698673","article-title":"Human Papillomavirus-related Carcinomas of the Sinonasal Tract","volume":"37","author":"Bishop","year":"2013","journal-title":"Am J Surg Pathology"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0514-4","article-title":"The Sinonasal Tract: Another Potential \u201cHot Spot\u201d for Carcinomas with Transcriptionally-Active Human Papillomavirus","volume":"8","author":"Lewis","year":"2014","journal-title":"Head Neck Pathology"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.32679","article-title":"Human Papillomavirus and Survival of Patients with Sinonasal Squamous Cell Carcinoma","volume":"126","author":"Oliver","year":"2020","journal-title":"Cancer"},{"key":"B37","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1186\/s41199-018-0039-3","article-title":"HPV-Driven Oropharyngeal Cancer: Current Knowledge of Molecular Biology and Mechanisms of Carcinogenesis","volume":"3","author":"Pan","year":"2018","journal-title":"Cancers Head Neck"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.03.003","article-title":"Understanding the HPV Integration and its Progression to Cervical Cancer","volume":"61","author":"Oyervides-Mu\u00f1oz","year":"2018","journal-title":"Infect Genet Evol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.149","article-title":"The Human Papillomavirus (HPV)-Related Cancer Biology: An Overview","volume":"106","author":"Araldi","year":"2018","journal-title":"BioMed Pharmacother"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.currproblcancer.2018.03.003","article-title":"HPV: Molecular Pathways and Targets","volume":"42","author":"Gupta","year":"2018","journal-title":"Curr Probl Cancer"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-016-1005-x","article-title":"The Molecular Mechanism of Human Papillomavirus-Induced Carcinogenesis in Head and Neck Squamous Cell Carcinoma","volume":"21","author":"Sano","year":"2016","journal-title":"Int J Clin Oncol"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2018.11","article-title":"The Molecular Landscape of Head and Neck Cancer","volume":"18","author":"Leemans","year":"2018","journal-title":"Nat Rev Cancer"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001540","article-title":"The Human Papillomavirus Oncoproteins: A Review of the Host Pathways Targeted on the Road to Transformation","volume":"102","author":"Scarth","year":"2021","journal-title":"J Gen Virol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0203403","article-title":"Differences in the Viral Genome Between HPV-Positive Cervical and Oropharyngeal Cancer","volume":"13","author":"LeConte","year":"2018","journal-title":"PloS One"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/j.semradonc.2021.02.006","article-title":"Biology of HPV Mediated Carcinogenesis and Tumor Progression","volume":"31","author":"Cosper","year":"2021","journal-title":"Semin Radiat Oncol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"2991","DOI":"10.1007\/s00405-017-4571-2","article-title":"Malignant Transformation of Sinonasal Inverted Papilloma and Related Genetic Alterations: A Systematic Review","volume":"274","author":"Re","year":"2017","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"B47","doi-asserted-by":"publisher","first-page":"S48","DOI":"10.1097\/pgp.0000000000000735","article-title":"The Silva Pattern-based Classification for HPV-associated Invasive Endocervical Adenocarcinoma and the Distinction Between In Situ and Invasive Adenocarcinoma: Relevant Issues and Recommendations From the International Society of Gynecological Pathologists","volume":"40","author":"Alvarado-Cabrero","year":"2021","journal-title":"Int J Gynecol Pathol"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1055\/a-1545-4279","article-title":"WHO Classification of Female Genital Tumors","volume":"81","author":"H\u00f6hn","year":"2021","journal-title":"Geburtsh Frauenheilk"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32412","article-title":"Identification of Prognostic Molecular Biomarkers in 157 HPV\u2010positive and HPV\u2010negative Squamous Cell Carcinomas of the Oropharynx","volume":"145","author":"Dogan","year":"2019","journal-title":"Int J Cancer"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0602-7","article-title":"Human Papillomavirus as a Driver of Head and Neck Cancers","volume":"122","author":"Sabatini","year":"2020","journal-title":"Br J Cancer"},{"key":"B51","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.humpath.2019.09.001","article-title":"The Evolving Landscape of HPV-Related Neoplasia in the Head and Neck","volume":"94","author":"Devins","year":"2019","journal-title":"Hum Pathol"},{"key":"B52","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1007\/s12105-016-0692-y","article-title":"Sinonasal Squamous Cell Carcinoma: A Review with Emphasis on Emerging Histologic Subtypes and the Role of Human Papillomavirus","volume":"10","author":"Lewis","year":"2016","journal-title":"Head Neck Pathol"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000000944","article-title":"HPV-Related Multiphenotypic Sinonasal Carcinoma","volume":"41","author":"Bishop","year":"2017","journal-title":"Am J Surg Pathol"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1002\/lary.28598","article-title":"HPV\u2010Related Multiphenotypic Sinonasal Carcinoma: A Case Report and Literature Review","volume":"131","author":"Ward","year":"2021","journal-title":"Laryngoscope"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0b013e31827b1cd6","article-title":"Human Papillomavirus\u2013related Carcinoma With Adenoid Cystic\u2013like Features","volume":"37","author":"Bishop","year":"2013","journal-title":"Am J Surg Pathol"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-017-2116-0","article-title":"New Tumor Entities in the 4th Edition of the World Health Organization Classification of Head and Neck Tumors: Nasal Cavity, Paranasal Sinuses and Skull Base","volume":"472","author":"Thompson","year":"2018","journal-title":"Virchows Arch"},{"key":"B57","doi-asserted-by":"publisher","first-page":"45","DOI":"10.3390\/cancers13010045","article-title":"Human Papillomavirus in Sinonasal Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis","volume":"13","author":"Pang","year":"2020","journal-title":"Cancers"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1097\/rmr.0000000000000288","article-title":"MRI of Sinonasal Malignancies","volume":"30","author":"Famuyide","year":"2021","journal-title":"Top Magn Reson Imag"},{"key":"B59","unstructured":"HPV Vaccine: Canadian Immunization Guide"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.11.020","article-title":"Cervical Cancer Screening of HPV Vaccinated Populations: Cytology, Molecular Testing, Both or None","volume":"76","author":"El-Zein","year":"2016","journal-title":"J Clin Virol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.4595","article-title":"Cervical Cancer Screening","volume":"321","author":"Sawaya","year":"2019","journal-title":"Jama"},{"key":"B62","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/978-3-642-68185-1","article-title":"Cervical Cytology as a Screening Method","volume":"70","author":"H\u00f6ffken","year":"1981","journal-title":"Curr Top Pathol"},{"key":"B63","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1002\/rmv.1822","article-title":"Human Papillomavirus Molecular Biology and Disease Association","volume":"25","author":"Doorbar","year":"2015","journal-title":"Rev Med Virol"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02896","article-title":"Involvement of Human Papillomaviruses in Cervical Cancer","volume":"9","author":"Wang","year":"2018","journal-title":"Front Microbiol"},{"key":"B65","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.bpobgyn.2020.02.008","article-title":"Primary HPV Screening for Cervical Cancer","volume":"65","author":"Bhatla","year":"2020","journal-title":"Best Pract Res Clin Obstet Gynaecol"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2014.11.076","article-title":"Primary Cervical Cancer Screening With Human Papillomavirus: End of Study Results From the ATHENA Study Using HPV as the First-Line Screening Test","volume":"136","author":"Wright","year":"2015","journal-title":"Gynecol Oncol"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.86a.18018","article-title":"Human Papillomavirus in 2019: An Update on Cervical Cancer Prevention and Screening Guidelines","volume":"86","author":"Zhang","year":"2019","journal-title":"Cleve Clin J Med"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30516","article-title":"Diagnostic Accuracy of P16ink4a Immunohistochemistry in Oropharyngeal Squamous Cell Carcinomas: A Systematic Review and Meta\u2010Analysis","volume":"140","author":"Prigge","year":"2017","journal-title":"Int J Cancer"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2011.09.002","article-title":"Is P16 Immunohistochemistry a More Cost-Effective Method for Identification of Human Papilloma Virus\u2013Associated Head and Neck Squamous Cell Carcinoma","volume":"16","author":"Thomas","year":"2012","journal-title":"Ann Diagn Pathol"},{"key":"B70","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4939-1516-3","volume-title":"Technical Aspects of Toxicological Immunohistochemistry, System Specific Biomarkers","author":"Aziz","year":"2016"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01751","article-title":"HPV Detection in Head and Neck Squamous Cell Carcinomas: What Is the Issue","volume":"10","author":"Augustin","year":"2020","journal-title":"Front Oncol"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12421","article-title":"Who Evaluates P16 Immunohistochemistry","volume":"123","author":"Larsen","year":"2015","journal-title":"Apmis"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22944","article-title":"Technical Review: In Situ Hybridization","volume":"297","author":"Jensen","year":"2014","journal-title":"Anat Rec"},{"key":"B74","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/s12105-012-0372-5","article-title":"HPV Detection Methods in Head and Neck Cancer","volume":"6","author":"Venuti","year":"2012","journal-title":"Head Neck Pathol"},{"key":"B75","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-1-62703-715-0","article-title":"The Polymerase Chain Reaction (PCR): General Methods","volume":"1099","author":"Waters","year":"2014","journal-title":"Methods Mol Biol"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.4065\/77.7.606","article-title":"Nobel Laureate for Procedure to Replicate DNA","volume":"77","author":"Shampo","year":"2002","journal-title":"Mayo Clin Proc"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1155\/2007\/591826","article-title":"International Standard Reagents for HPV Detection","volume":"23","author":"Pagliusi","year":"2007","journal-title":"Dis Markers"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2012.04.001","article-title":"Detection of Human Papillomavirus in Clinical Samples","volume":"45","author":"Westra","year":"2012","journal-title":"Otolaryngol Clin North Am"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1097\/pap.0b013e31828d1893","article-title":"Human Papillomavirus Detection: Testing Methodologies and Their Clinical Utility in Cervical Cancer Screening","volume":"20","author":"Laudadio","year":"2013","journal-title":"Adv Anatomic Pathol"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2014.05.004","article-title":"Detection of Human Papillomavirus (HPV) in Clinical Samples: Evolving Methods and Strategies for the Accurate Determination of HPV Status of Head and Neck Carcinomas","volume":"50","author":"Westra","year":"2014","journal-title":"Oral Oncol"},{"key":"B81","year":"2017","journal-title":"HOLOGIC Aptima HPV Assay"},{"key":"B82","unstructured":"OncoE6TM Cervical Test Instructions for Use.2020"},{"key":"B83","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s12905-018-0559-3","article-title":"Performance of OncoE6 Cervical Test With Collection Methods Enabling Self-Sampling","volume":"18","author":"Krings","year":"2018","journal-title":"Bmc Women\u2019s Heal"},{"key":"B84","doi-asserted-by":"publisher","first-page":"1453","DOI":"10.1002\/ijc.29877","article-title":"Effectiveness of Novel, Lower Cost Molecular Human Papillomavirus\u2010Based Tests for Cervical Cancer Screening in Rural China","volume":"138","author":"Valdez","year":"2016","journal-title":"Int J Cancer"},{"key":"B85","doi-asserted-by":"publisher","first-page":"e0201262","DOI":"10.1371\/journal.pone.020126","article-title":"Self-Sampling Coupled to the Detection of HPV 16 and 18 E6 Protein: A Promising Option for Detection of Cervical Malignancies in Remote Areas","volume":"13","author":"Torres","year":"2018","journal-title":"Plos One"},{"key":"B86","doi-asserted-by":"publisher","DOI":"10.1097\/pas.0000000000001410","article-title":"HPV-Related Sinonasal Carcinoma","volume":"44","author":"Jiromaru","year":"2020","journal-title":"Am J Surg Pathol"},{"key":"B87","doi-asserted-by":"publisher","DOI":"10.4149\/neo_2020_200330n332","article-title":"Significance of Transcriptionally-Active High-Risk Human Papillomavirus in Sinonasal Squamous Cell Carcinoma: Case Series and a Meta-Analysis","volume":"67","author":"\u0160vajdler","year":"2021","journal-title":"Neoplasma"},{"key":"B88","doi-asserted-by":"publisher","DOI":"10.1002\/lary.29184","article-title":"Detection of HPV16\/18 E6 Oncoproteins in Head and Neck Squamous Cell Carcinoma Using a Protein Immunochromatographic Assay","volume":"131","author":"Menegaldo","year":"2021","journal-title":"Laryngoscope"},{"key":"B89","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.pvr.2018.05.003","article-title":"HPV E6 Oncoproteins and Nucleic Acids in Neck Lymph Node Fine Needle Aspirates and Oral Samples From Patients With Oropharyngeal Squamous Cell Carcinoma","volume":"6","author":"Chernesky","year":"2018","journal-title":"Papillomavirus Res"},{"key":"B90","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1200\/po.18.00276","article-title":"Detection of Early Human Papillomavirus\u2013Associated Cancers by Liquid Biopsy","volume":"3","author":"Damerla","year":"2019","journal-title":"JCO Precis Oncol"},{"key":"B91","doi-asserted-by":"publisher","first-page":"clincanres.0625.2021","DOI":"10.1158\/1078-0432.ccr-21-0625","article-title":"Circulating HPV DNA as a Marker for Early Detection of Relapse in Patients With Cervical Cancer","volume":"27","author":"Jeannot","year":"2021","journal-title":"Clin Cancer Res"},{"key":"B92","doi-asserted-by":"publisher","DOI":"10.1002\/cjp2.47","article-title":"Circulating Human Papillomavirus DNA Detected Using Droplet Digital PCR in the Serum of Patients Diagnosed With Early Stage Human Papillomavirus\u2010Associated Invasive Carcinoma","volume":"2","author":"Jeannot","year":"2016","journal-title":"J Pathol Clin Res"},{"key":"B93","unstructured":"Droplet DigitalTM PCR Applications Guide2011"},{"key":"B94","doi-asserted-by":"publisher","first-page":"2409","DOI":"10.1038\/s41598-017-02217-x","article-title":"Droplet Digital PCR Versus qPCR for Gene Expression Analysis With Low Abundant Targets: From Variable Nonsense to Publication Quality Data","volume":"7","author":"Taylor","year":"2017","journal-title":"Sci Rep-uk"},{"key":"B95","doi-asserted-by":"publisher","first-page":"BSR20181170","DOI":"10.1042\/bsr20181170","article-title":"Application of Droplet Digital PCR to Detect the Pathogens of Infectious Diseases","volume":"38","author":"Li","year":"2018","journal-title":"Biosci Rep"},{"key":"B96","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114193","article-title":"Optimization of Droplet Digital PCR Assays for the Type-Specific Detection and Quantification of Five HPV Genotypes, Including Additional Data on Viral Loads of Nine Different HPV Genotypes in Cervical Carcinomas","volume":"294","author":"Malin","year":"2021","journal-title":"J Virol Methods"},{"key":"B97","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-017-0331-y","article-title":"Digital Droplet PCR (ddPCR) for the Detection and Quantification of HPV 16, 18, 33 and 45 - a Short Report","volume":"40","author":"Larsson","year":"2017","journal-title":"Cell Oncol (Dordr)"},{"key":"B98","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1007\/s40291-020-00502-6","article-title":"Human Papillomavirus DNA Detection by Droplet Digital PCR in Formalin-Fixed Paraffin-Embedded Tumor Tissue From Oropharyngeal Squamous Cell Carcinoma Patients","volume":"25","author":"Schiavetto","year":"2021","journal-title":"Mol Diagn Ther"},{"key":"B99","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/00016489.2018.1461239","article-title":"HPV-16 Viral Load in Oropharyngeal Squamous Cell Carcinoma Using Digital PCR","volume":"138","author":"Antonsson","year":"2018","journal-title":"Acta Otolaryngol"},{"key":"B100","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10030143","article-title":"Superiority of Droplet Digital PCR Over Real-Time Quantitative PCR for JAK2 V617F Allele Mutational Burden Assessment in Myeloproliferative Neoplasms: A Retrospective Study","volume":"10","author":"Rocca","year":"2020","journal-title":"Diagnostics"},{"key":"B101","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1007\/s40944-021-00539-3","article-title":"Implementation of HPV Self-Sampling in Cervical Screening Programs Increases Participation Rates: A Literature Review","volume":"19","author":"Schmeink","year":"2021","journal-title":"Indian J Gynecol Oncol"},{"key":"B102","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29026","article-title":"Comparative Performance of Novel Self\u2010Sampling Methods in Detecting High\u2010Risk Human Papillomavirus in 30,130 Women Not Attending Cervical Screening","volume":"136","author":"Bosgraaf","year":"2015","journal-title":"Int J Cancer"},{"key":"B103","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28283","article-title":"HPV Self\u2010Sampling or the Pap\u2010smear: A Randomized Study Among Cervical Screening Nonattenders From Lower Socioeconomic Groups in France","volume":"133","author":"Sancho\u2010Garnier","year":"2013","journal-title":"Int J Cancer"},{"key":"B104","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1038\/bjc.2017.485","article-title":"Randomised Study Shows That Repeated Self-Sampling and HPV Test has More Than Two-Fold Higher Detection Rate of Women With CIN2+ Histology Than Pap Smear Cytology","volume":"118","author":"Gustavsson","year":"2018","journal-title":"Br J Cancer"},{"key":"B105","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-011754","article-title":"Community-Randomised Controlled Trial Embedded in the Anishinaabek Cervical Cancer Screening Study: Human Papillomavirus Self-Sampling Versus Papanicolaou Cytology","volume":"6","author":"Zehbe","year":"2016","journal-title":"BMJ Open"},{"key":"B106","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s12905-019-0778-2","article-title":"Perspectives of Women Participating in a Cervical Cancer Screening Campaign With Community-Based HPV Self-Sampling in Rural Western Kenya: A Qualitative Study","volume":"19","author":"Oketch","year":"2019","journal-title":"BMC Womens Health"},{"key":"B107","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12549","article-title":"Uptake of Community\u2010Based, Self\u2010Collected HPV Testing vs. Visual Inspection With Acetic Acid for Cervical Cancer Screening in Kampala, Uganda: Preliminary Results of a Randomised Controlled Trial","volume":"20","author":"Moses","year":"2015","journal-title":"Trop Med Int Health"},{"key":"B108","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2014.510","article-title":"Vaginal Self-Sampling is a Cost-Effective Way to Increase Participation in a Cervical Cancer Screening Programme: A Randomised Trial","volume":"111","author":"Haguenoer","year":"2014","journal-title":"Br J Cancer"}],"container-title":["Frontiers in Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2022.864820\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T05:04:59Z","timestamp":1651035899000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2022.864820\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,27]]},"references-count":108,"alternative-id":["10.3389\/fonc.2022.864820"],"URL":"https:\/\/doi.org\/10.3389\/fonc.2022.864820","relation":{},"ISSN":["2234-943X"],"issn-type":[{"value":"2234-943X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,27]]},"article-number":"864820"}}