{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T13:36:49Z","timestamp":1773063409902,"version":"3.50.1"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>High-grade serous ovarian cancer (HGSOC) remains the most lethal female cancer due to metastasis. CircRNAs are recently identified to be modified by N6-methyladenosine (m<jats:sup>6<\/jats:sup>A) in many cells. However, the significance of m<jats:sup>6<\/jats:sup>A-modified circular RNAs (circRNAs) has not been elucidated in HGSOC peritoneal metastasis. Here, we aimed to investigate the participation and potential functions of m<jats:sup>6<\/jats:sup>A-modified circRNAs in HGSCO peritoneal metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cancerous tissues were collected from the <jats:italic>in situ<\/jats:italic> and the peritoneal metastasis lesions of HGSCO patients. M<jats:sup>6<\/jats:sup>A-tagged circRNAs were identified by m<jats:sup>6<\/jats:sup>A-modified RNA immunoprecipitation sequencing (m<jats:sup>6<\/jats:sup>A-RIP-seq). Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the potential functions of the m<jats:sup>6<\/jats:sup>A-modified circRNAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For the m<jats:sup>6<\/jats:sup>A-modified circRNAs, 259 were upregulated and 227 were downregulated in the peritoneal metastasis than in the situ lesions of HGSCO patients. For the m<jats:sup>6<\/jats:sup>A peaks, 1541 were upregulated and 1293 were downregulated in the peritoneal metastasis than in the <jats:italic>in situ<\/jats:italic> lesions of HGSCO patients. For the differential expressed circRNAs, 1911(19.6%) were upregulated and 2883(29.6%) were downregulated in the peritoneal metastasis than in the <jats:italic>in situ<\/jats:italic> lesions of HGSCO patients. The upregulated m<jats:sup>6<\/jats:sup>A-modified circRNAs were associated with the HIF-1 signaling. The downregulated m<jats:sup>6<\/jats:sup>A-modified circRNAs were associated with the MAPK signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This work firstly identified the transcriptome-wide map of m<jats:sup>6<\/jats:sup>A-modified circRNAs in peritoneal metastasis of HGSCO. Our findings provided novel evidences about the participation of m<jats:sup>6<\/jats:sup>A-modified circRNAs <jats:italic>via<\/jats:italic> HIF-1 and MAPK signaling and a new insight in molecular target of HGSCO peritoneal metastasis.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2022.988578","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T08:42:37Z","timestamp":1663663357000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Comprehensive analysis of m6A-modified circRNAs in peritoneal metastasis of high grade serious carcinoma of ovary"],"prefix":"10.3389","volume":"12","author":[{"given":"Lin","family":"Guo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nini","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daner","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaozhe","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shasha","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1038\/s41419-021-04256-9","article-title":"ETV5-mediated upregulation of lncRNA CTBP1-DT as a ceRNA facilitates HGSOC progression by regulating miR-188-5p\/MAP3K3 axis","volume":"12","author":"Liu","year":"2021","journal-title":"Cell Death Dis"},{"key":"B2","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/s13045-020-0844-0","article-title":"MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC","volume":"13","author":"Sun","year":"2020","journal-title":"J Hematol Oncol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3322\/caac.21654","article-title":"Cancer statistics, 2021","volume":"71","author":"Siegel","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-21-2984","article-title":"Metabolic alterations and WNT signaling impact immune response in HGSOC","volume":"28","author":"Arend","year":"2022","journal-title":"Clin Cancer Res"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/nature10166","article-title":"The Cancer Genome Atlas Research Network. 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