{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:43:52Z","timestamp":1791233032422,"version":"4.1.0"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>It has been established that the scavenger receptor class A member 5 (<jats:italic>SCARA5<\/jats:italic>) functions as a tumor suppressor gene in various cancer types. To our knowledge, no comprehensive study has hitherto investigated the expression and function of <jats:italic>SCARA5<\/jats:italic> in melanoma. This study aimed to determine the association between <jats:italic>SCARA5<\/jats:italic> and melanoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Analysis of <jats:italic>SCARA5<\/jats:italic> mRNA expression was performed using The Cancer Genome Atlas (TCGA) data sets. To evaluate the clinical significance of <jats:italic>SCARA5<\/jats:italic>, the clinical data of 93 patients with melanoma were collected. The role of <jats:italic>SCARA5<\/jats:italic> expression in prognosis was also analyzed. In this study, survival was evaluated by Kaplan\u2013Meier analysis and compared using the log-rank test. Univariate and multivariate Cox proportional hazard regression analyses were used to identify independent predictors. The Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and gene set enrichment analysis (GSEA) were used to perform gene set functional annotations. Protein\u2013protein interaction (PPI) networks were constructed to illustrate gene\u2013gene interactions. The Tumor IMmune Estimation Resource (TIMER) database was used to explore the association between <jats:italic>SCARA5<\/jats:italic> and immune infiltration levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that the <jats:italic>SCARA5<\/jats:italic> mRNA expression in melanoma was significantly lower than in adjacent normal skin tissue (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). Moreover, decreased expression of <jats:italic>SCARA5<\/jats:italic> in melanoma correlated with the tumor, node, and metastasis (TNM) stage and recurrence (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). The overall survival (OS) was significantly higher in melanoma with high <jats:italic>SCARA5<\/jats:italic> expression compared with low <jats:italic>SCARA5<\/jats:italic> expression (<jats:italic>p<\/jats:italic> &amp;lt; 0.001). During univariate analysis, <jats:italic>SCARA5<\/jats:italic> expression, tumor (T) stage, node (N) stage, metastasis (M) stage, and recurrence correlated with OS (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Further multivariate Cox regression analysis showed that <jats:italic>SCARA5<\/jats:italic> expression (<jats:italic>p<\/jats:italic> = 0.012) could be an independent prognostic factor for OS in cutaneous malignant melanoma. GSEA analysis showed that <jats:italic>SCARA5<\/jats:italic> was significantly enriched in various pathways, such as response to developmental biology and response to antimicrobial peptides. Correlation analysis showed a positive correlation with CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells (<jats:italic>p<\/jats:italic> &amp;lt; 0.05), and a negative correlation with tumor purity (<jats:italic>p<\/jats:italic> &amp;lt; 0.05)<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>SCARA5<\/jats:italic> has significant potential as a prognostic biomarker and as a promising therapeutic target in melanoma. Furthermore, <jats:italic>SCARA5<\/jats:italic> expression in melanoma is related to the level of immune infiltration.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2023.1015358","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T05:23:16Z","timestamp":1679635396000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Decreased expression of SCARA5 predicts a poor prognosis in melanoma using bioinformatics 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