{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:11:41Z","timestamp":1792606301468,"version":"4.5.0"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>We investigated HPV genotypes in a large cohort of patients with definitive cervical histologic diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HPV testing was performed by real-time PCR assay, including 18 high-risk HPV (hrHPV) and 3 low-risk HPV (lrHPV). Totally 61,422 patients with documented HPV genotyping results within 6 months before cervical histologic diagnoses were included.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HrHPV positive rate was 55.1% among all tested cases with the highest in adenosquamous carcinoma (94.1%), followed by squamous cell carcinoma (SCC) (93.7%), cervical intraepithelial neoplasia 2\/3 (CIN2\/3) (92.8%). HrHPV positive rates were significantly higher in high-grade squamous lesions than in those in glandular lesions. HPV16 was the most common genotype followed by HPV52 and HPV58 in CIN2\/3. The most frequent hrHPV genotype in adenocarcinoma <jats:italic>in situ<\/jats:italic> (AIS) was HPV18, followed by HPV16, HPV45 and HPV52. In SCC cases, HPV16 was the most common type followed by HPV58, HPV52, HPV18 and HPV33. However, HPV18 showed significantly higher prevalence in adenocarcinoma and adenosquamous carcinoma than in that in SCC. Theoretically, the protective rates of 2\/4-valent and 9-valent vaccine were 69.1% and 85.8% for cervical cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The prevalence of HPV genotypes in Chinese population was different from that in Western population. Some hrHPV types were identified in cervical precancerous lesions and cancers, which are not included in current HPV vaccines. These data provide baseline knowledge for future HPV vaccine development.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2023.1161631","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T06:13:27Z","timestamp":1680070407000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["HPV genotyping of cervical histologic specimens of 61, 422 patients from the largest women hospital in China"],"prefix":"10.3389","volume":"13","author":[{"given":"Fangfang","family":"Zhong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaibo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihua","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoxing","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianrong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Cong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Sui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengquan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01518-19","article-title":"Clinical and analytical performance of the BD onclarity HPV assay with SurePath screening samples from the danish cervical screening program using the VALGENT framework","volume":"58","author":"Bonde","year":"2020","journal-title":"J Clin Microbiol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1604621","DOI":"10.3389\/ijph.2022.1604621","article-title":"Is it time to genotype beyond HPV16 and HPV18 for cervical cancer screening","volume":"67","author":"Chua","year":"2022","journal-title":"Int J Public Health"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8","article-title":"Human papillomavirus genotype attribution in invasive cervical cancer: A retrospective cross-sectional worldwide study","volume":"11","author":"de Sanjose","year":"2010","journal-title":"Lancet Oncol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0b013e3182a577c7","article-title":"Prevalence of human papillomavirus types in invasive cervical cancers from 7 US cancer registries before vaccine introduction","volume":"18","author":"Hopenhayn","year":"2014","journal-title":"J Low Genit Tract Dis"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2020.1805993","article-title":"Prevalence and genotype distribution of human papillomavirus among women with cervical lesions in shenzhen city, china","volume":"17","author":"Mai","year":"2021","journal-title":"Hum Vaccin Immunother"},{"key":"B7","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s00705-017-3556-1","article-title":"Human papillomavirus genotype distribution among HPV-positive women in sichuan province, southwest china","volume":"163","author":"Tao","year":"2018","journal-title":"Arch Virol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2021.710209","article-title":"And genotype distribution among women from hengyang district of hunan province, china","volume":"9","author":"Tang","year":"2021","journal-title":"Front Public Health"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22536","article-title":"Risk stratification for cervical neoplasia using extended high-risk HPV genotyping in women with ASC-US cytology: A large retrospective study from china","volume":"130","author":"Tao","year":"2022","journal-title":"Cancer Cytopathol"},{"key":"B10","volume-title":"WHO classification of tumours: Female genital tumours","year":"2020"},{"key":"B11","first-page":"255","article-title":"Human papillomaviruses","year":"2012","journal-title":"IARC Monogr Eval Carcinog Risks Hum"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2019.05.012","article-title":"Risk detection for high-grade cervical disease using onclarity HPV extended genotyping in women, >\/=21years of age, with ASC-US or LSIL cytology","volume":"154","author":"Wright","year":"2019","journal-title":"Gynecol Oncol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqy084","article-title":"Detection of cervical neoplasia by human papillomavirus testing in an atypical squamous cells-undetermined significance population: Results of the becton dickinson onclarity trial","volume":"151","author":"Wright","year":"2019","journal-title":"Am J Clin Pathol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasc.2021.03.006","article-title":"Prevalence and carcinogenic risk of high-risk human papillomavirus subtypes in different cervical cytology: a study of 124,251 cases from the largest academic center in china","volume":"10","author":"Tao","year":"2021","journal-title":"J Am Soc Cytopathol"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.2147\/RMHP.S384342","article-title":"Assessment of high-risk human papillomavirus infection characteristics in cervical squamous cell carcinoma and adenocarcinoma in china","volume":"15","author":"Zhuang","year":"2022","journal-title":"Risk Manag Healthc Policy"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001800","article-title":"The prognostic values of HPV genotypes and tumor PD-L1 expression in patients with HPV-associated endocervical adenocarcinoma","volume":"46","author":"Zhou","year":"2022","journal-title":"Am J Surg Pathol"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28302","article-title":"Extended human papillomavirus genotype distribution in cervical intraepithelial neoplasia and cancer: Analysis of 40\u2009352 cases from a large academic gynecologic center in china","volume":"2022","author":"Tang","year":"2022","journal-title":"J Med Virol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0276438","article-title":"Prevalence of anal dysplasia and HPV genotypes in gynecology patients: The ANGY cross-sectional prospective clinical study protocol","volume":"17","author":"Pache","year":"2022","journal-title":"PloS One"},{"key":"B19","doi-asserted-by":"publisher","first-page":"960","DOI":"10.3390\/pathogens11090960","article-title":"Prevalence, diversity, and risk factors for cervical HPV infection in women screened for cervical cancer in bel\u00e9m, par\u00e1, northern brazil","volume":"11","author":"Monteiro","year":"2022","journal-title":"Pathogens"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/s10552-022-01638-y","article-title":"Cervical cancer in ethiopia: A review of the literature","volume":"34","author":"Derbie","year":"2023","journal-title":"Cancer Causes Control"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2021.211004","article-title":"The frequency and typing of HPV virus among suspected women referred to HPV genotyping test in mazandaran, northern iran","volume":"68","author":"Hosseini","year":"2022","journal-title":"Clin Lab"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2022.23.7.2477","article-title":"Genotype distribution and prevalence of high-risk human papillomavirus infection among women in samsun province of turkey","volume":"23","author":"Taskin","year":"2022","journal-title":"Asian Pac J Cancer Prev"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijregi.2021.10.005","article-title":"Genotypic distribution and prevalence of human papillomavirus infection in an apparently healthy female population in bangladesh","volume":"1","author":"Sharmin","year":"2021","journal-title":"IJID Reg"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0257915","article-title":"Genotype distribution of cervical HPV among caribbean women in a population-based study in martinique: The DEPIPAPUFR study","volume":"16","author":"Najioullah","year":"2021","journal-title":"PloS One"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1177\/10732748221103331","article-title":"Prevalence and distribution of human papillomavirus genotypes (1997-2019) and their association with cervical cancer and precursor lesions in women from southern mexico","volume":"29","author":"Alarcon-Romero","year":"2022","journal-title":"Cancer Control"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2017.10.031","article-title":"HPV genotype profile in a norwegian cohort with ASC-US and LSIL cytology with three year cumulative risk of high grade cervical neoplasia","volume":"148","author":"Lie","year":"2018","journal-title":"Gynecol Oncol"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2020.1814097","article-title":"Human papillomavirus genotype-specific risks for cervical intraepithelial lesions","volume":"17","author":"Nygard","year":"2021","journal-title":"Hum Vaccin Immunother"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.currproblcancer.2021.100761","article-title":"Characterization of HPV subtypes not covered by the nine-valent vaccine in patients with CIN 2-3 and cervical squamous cell carcinoma","volume":"45","author":"Ma","year":"2021","journal-title":"Curr Probl Cancer"},{"key":"B29","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1186\/s12885-022-09826-4","article-title":"The value of HPV genotypes combined with clinical indicators in the classification of cervical squamous cell carcinoma and adenocarcinoma","volume":"22","author":"He","year":"2022","journal-title":"BMC Cancer"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268821000741","article-title":"Association of human papillomavirus genotype distribution and cervical cytology: A cross-sectional study","volume":"149","author":"Liu","year":"2021","journal-title":"Epidemiol Infect"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000155","article-title":"Multiple papillomavirus infection and size of colposcopic lesions among women with cervical intraepithelial neoplasia","volume":"20","author":"Spinillo","year":"2016","journal-title":"J Low Genit Tract Dis"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2016.10.006","article-title":"Is incidence of multiple HPV genotypes rising in genital infections","volume":"10","author":"Sohrabi","year":"2017","journal-title":"J Infect Public Health"},{"key":"B33","doi-asserted-by":"publisher","first-page":"380","DOI":"10.3390\/v12040380","article-title":"Multiple HPV infections and viral load association in persistent cervical lesions in mexican women","volume":"12","author":"Oyervides-Munoz","year":"2020","journal-title":"Viruses"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-3835(03)00312-4","article-title":"Multiple HPV infection in cervical cancer screened by HPVDNAChip","volume":"198","author":"Lee","year":"2003","journal-title":"Cancer Lett"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.3390\/v13071342","article-title":"Multiple human papilloma virus (HPV) infections are associated with HSIL and persistent HPV infection status in korean patients","volume":"13","author":"Kim","year":"2021","journal-title":"Viruses"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.22034\/APJCP.2018.19.3.785","article-title":"Possible synergistic interactions among multiple HPV genotypes in women suffering from genital neoplasia","volume":"19","author":"Hajia","year":"2018","journal-title":"Asian Pac J Cancer Prev"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq058","article-title":"The influence of multiple human papillomavirus types on the risk of genotype-concordant incident infections of the anus and cervix: The hawaii HPV cohort study","volume":"203","author":"Goodman","year":"2011","journal-title":"J Infect Dis"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2019.0189","article-title":"Identification of multiple high-risk human papillomavirus infections in a rural population of canatlan, durango, mexico","volume":"24","author":"Favela-Bueno","year":"2020","journal-title":"Genet Test Mol Biomarkers"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23143","article-title":"Genotypes and prevalence of HPV single and multiple concurrent infections in women with HSIL","volume":"42","author":"Beca","year":"2014","journal-title":"Diagn Cytopathol"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyu112","article-title":"Human papillomavirus genotype distribution in cervical intraepithelial neoplasia grade 2\/3 and invasive cervical cancer in japanese women","volume":"44","author":"Azuma","year":"2014","journal-title":"Jpn J Clin Oncol"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2020.05.009","article-title":"Preferential expression of a HPV genotype in invasive cervical carcinomas infected by multiple genotypes","volume":"112","author":"Brant","year":"2020","journal-title":"Genomics"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2011.06.010","article-title":"HPV genotyping in neuroendocrine carcinoma of the uterine cervix in northern thailand","volume":"115","author":"Siriaunkgul","year":"2011","journal-title":"Int J Gynaecol Obstet"},{"key":"B43","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1186\/s13000-018-0709-9","article-title":"Cervical small cell carcinoma frequently presented in multiple high risk HPV infection and often associated with other type of epithelial tumors","volume":"13","author":"Li","year":"2018","journal-title":"Diagn Pathol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqac073","article-title":"The clinical utility of extended high-risk HPV genotyping in women with ASC-US cytology","volume":"158","author":"Jiang","year":"2022","journal-title":"Am J Clin Pathol"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1002\/cncy.22573","article-title":"The clinical utility of extended high-risk HPV genotyping in risk-stratifying women with l-SIL cytology: A retrospective study of 8726 cases","volume":"130","author":"Tao","year":"2022","journal-title":"Cancer Cytopathol"}],"container-title":["Frontiers in Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2023.1161631\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T06:13:30Z","timestamp":1680070410000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2023.1161631\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,29]]},"references-count":45,"alternative-id":["10.3389\/fonc.2023.1161631"],"URL":"https:\/\/doi.org\/10.3389\/fonc.2023.1161631","relation":{},"ISSN":["2234-943X"],"issn-type":[{"value":"2234-943X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,29]]},"article-number":"1161631"}}