{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T16:03:11Z","timestamp":1788624191215,"version":"build-2803163510"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016-07168-2, 2015\/26079-8"],"award-info":[{"award-number":["2016-07168-2, 2015\/26079-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["1673350"],"award-info":[{"award-number":["1673350"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Bisphosphonates (BFs) show clinical effectiveness in managing osteoporosis and bone metastases but pose risks of bisphosphonate-related jaw osteonecrosis (BRONJ). With no established gold standard for BRONJ treatment, our focus is on symptom severity reduction. We aimed to assess the preventive effects of bioactive glass and\/or pericardial membrane in a preclinical BRONJ model, evaluating their potential to prevent osteonecrosis and bone loss post-tooth extractions in zoledronic acid (ZA)-treated animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rats, receiving ZA or saline biweekly for four weeks, underwent 1st and 2nd lower left molar extractions. Pericardial membrane alone or with F18 bioglass was applied post-extractions. Microarchitecture analysis and bone loss assessment utilized computerized microtomography (CT) and positron emission tomography (PET) with 18F-FDG and 18F-NaF tracers. Histological analysis evaluated bone injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exclusive alveolar bone loss occurred post-extraction in the continuous ZA group, inducing osteonecrosis, osteolysis, osteomyelitis, and abscess formation. Concurrent pericardial membrane with F18 bioglass application prevented these outcomes. Baseline PET\/CT scans showed no discernible uptake differences, but post-extraction 18F-FDG tracer imaging revealed heightened glucose metabolism at the extraction site in the ZA-treated group with membrane, contrasting the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest pericardial membrane with F18 bioglass effectively prevents BRONJ in the preclinical model.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2024.1348118","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T04:38:50Z","timestamp":1715315930000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Mitigating jaw osteonecrosis: bioactive glass and pericardial membrane combination in a rat model"],"prefix":"10.3389","volume":"14","author":[{"given":"Alexandre Antonio","family":"Pellicano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernar M.","family":"Benites","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda F. N.","family":"Paschoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura C.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina P.","family":"Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel O.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline C.","family":"Real","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"de Paula Faria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe P.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel R. C.","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosana L.","family":"Pagano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo R.","family":"Fregnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2014.04.031","article-title":"American association of oral and maxillofacial surgeons position paper on medication-related osteonecrosis of the jaw\u20142014 update","volume":"72","author":"Ruggiero","year":"2014","journal-title":"J Oral Maxillofac Surgery"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.23324","article-title":"Medication-related osteonecrosis of the jaws (MRONJ) in cancer patients treated with denosumab VS. zoledronic acid: A systematic review and meta-analysis","volume":"25","author":"Limones","year":"2020","journal-title":"Medicina Oral Patolog\u00eda Oral y Cirugia Bucal"},{"key":"B3","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1634\/theoncologist.9-90004-28","article-title":"Safety of intravenous and oral bisphosphonates and compliance with dosing regimens","volume":"9","author":"Conte","year":"2004","journal-title":"Oncologist"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.coms.2015.06.008","article-title":"Diagnosis and staging of medication-related osteonecrosis of the jaw","volume":"27","author":"Ruggiero","year":"2015","journal-title":"Oral Maxillofac Surg Clinics North America"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.23191","article-title":"Review and update on drugs related to the development of osteonecrosis of the jaw","volume":"25","author":"Eguia","year":"2020","journal-title":"Medicina Oral Patolog\u00eda Oral y Cirugia Bucal"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1200\/jco.19.01186","article-title":"Medication-related osteonecrosis of the jaw: MASCC\/ISOO\/ASCO clinical practice guideline","volume":"37","author":"Yarom","year":"2019","journal-title":"J Clin Oncol Off J Am Soc Clin Oncol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b16-01020","article-title":"Underlying mechanisms and therapeutic strategies for bisphosphonate-related osteonecrosis of the jaw (BRONJ)","volume":"40","author":"Endo","year":"2017","journal-title":"Biol Pharm bulletin"},{"key":"B8","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.jocd.2016.09.005","article-title":"Case-based review of osteonecrosis of the jaw (ONJ) and application of the international recommendations for management from the international task force on ONJ","volume":"20","author":"Khan","year":"2017","journal-title":"J Clin Densitometry"},{"key":"B9","doi-asserted-by":"publisher","first-page":"49","DOI":"10.23736\/S2724-6329.20.04306-X","article-title":"Bisphosphonate-associated osteonecrosis of the jaw","volume":"70","author":"Ferreira Jr.","year":"2021","journal-title":"Minerva Dental Oral science"},{"key":"B10","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1186\/s40001-023-01095-6","article-title":"Clinical values of serum Semaphorin 4D (Sema4D) in medication\u2212related osteonecrosis of the jaw","volume":"28","author":"Mu","year":"2023","journal-title":"Eur J Med Res"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2016.06.011","article-title":"Osteonecrosis of the jaws (ONJ) in mice after extraction of teeth with periradicular disease","volume":"90","author":"Soundia","year":"2016","journal-title":"Bone"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.sdentj.2021.12.006","article-title":"Treatment of bisphosphonate induced osteonecrosis of jaw in rats using an angiogenesis factor (A-Heal) and ABMDO (Autologous Bone Marrow Derived Osteoblasts)","volume":"34","author":"Sadat-Ali","year":"2022","journal-title":"Saudi Dental J"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-021-00890-9","article-title":"Zoledronic acid deteriorates soft and hard tissue healing of murine tooth extraction sockets in a dose-dependent manner","volume":"110","author":"Kozutsumi","year":"2022","journal-title":"Calcified Tissue Int"},{"key":"B14","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/s41598-021-03966-6","article-title":"Osteonecrosis development by tooth extraction in zoledronate treated mice is inhibited by active vitamin D analogues, anti-inflammatory agents or antibiotics","volume":"12","author":"Soma","year":"2022","journal-title":"Sci Rep"},{"key":"B15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2018\/3982540","article-title":"Conservative surgical treatment of bisphosphonate-related osteonecrosis of the jaw with er,Cr:YSGG laser and platelet-rich plasma: A longitudinal study","volume":"2018","author":"Mauceri","year":"2018","journal-title":"BioMed Res Int"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2012.04.001","article-title":"What is the role of hyperbaric oxygen in the management of bisphosphonate-related osteonecrosis of the jaw: A randomized controlled trial of hyperbaric oxygen as an adjunct to surgery and antibiotics","volume":"70","author":"Freiberger","year":"2012","journal-title":"J Oral Maxillofac Surgery"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2011.02.078","article-title":"Bisphosphonate-related osteonecrosis of the jaws\u2013an initial case series report of treatment combining partial bone resection and autologous platelet-rich plasma","volume":"69","author":"Curi","year":"2011","journal-title":"J Oral Maxillofac Surgery"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2017.03.037","article-title":"Development of a bioactive glass-polymer composite for wound healing applications","volume":"76","author":"Moura","year":"2017","journal-title":"Materials Sci Engineering: C"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2020.111475","article-title":"Bactericidal activity and biofilm inhibition of F18 bioactive glass against Staphylococcus aureus","volume":"118","author":"Passos","year":"2021","journal-title":"Materials Sci Engineering: C"},{"key":"B20","doi-asserted-by":"publisher","first-page":"400","DOI":"10.3390\/ma11030400","article-title":"Electrospun F18 bioactive glass\/PCL-poly (\u03f5-caprolactone)-membrane for\u00a0guided tissue regeneration","volume":"11","author":"Hidalgo Pitaluga","year":"2018","journal-title":"Materials (Basel Switzerland)"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1097\/00006123-199610000-00025","article-title":"Bovine pericardium for dural grafts: clinical results in 35 patients","volume":"39","author":"Anson","year":"1996","journal-title":"Neurosurgery"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(91)90065-I","article-title":"Comparative evaluation of treated bovine pericardium as a xenograft for hernia repair","volume":"12","author":"James","year":"1991","journal-title":"Biomaterials"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1097\/00002341-200109000-00014","article-title":"Bovine pericardium as a wrapping for orbital implants","volume":"17","author":"Gayre","year":"2001","journal-title":"Ophthalmic Plast Reconstructive Surgery"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.04.013","article-title":"Bony changes in the jaws of rats treated with zoledronic acid and dexamethasone before dental extractions mimic bisphosphonate-related osteonecrosis in cancer patients","volume":"45","author":"Sonis","year":"2009","journal-title":"Oral Oncol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-019-03054-0","article-title":"A modified protocol of mandibular osteoradionecrosis induction in rats with external beam radiation therapy","volume":"24","author":"Yamasaki","year":"2020","journal-title":"Clin Oral Investigations"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.119726","article-title":"How to calculate sample size in animal studies","volume":"4","author":"Charan","year":"2013","journal-title":"J Pharmacol Pharmacotherapeutics"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.6944","article-title":"Medication-related osteonecrosis of the jaw: A review","volume":"12","author":"AlDhalaan","year":"2020","journal-title":"Cureus"},{"key":"B28","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/s12254-012-0328-1","article-title":"Drug-induced osteonecrosis of the jaw","volume":"5","author":"Bergmeister","year":"2012","journal-title":"memo - Magazine Eur Med Oncol"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcms.2020.02.017","article-title":"Medication-related osteonecrosis of the jaw (MRONJ) stage III: Conservative and conservative surgical approaches versus an aggressive surgical intervention: A systematic review","volume":"48","author":"Vanpoecke","year":"2020","journal-title":"J Cranio-Maxillofacial Surgery"},{"key":"B30","doi-asserted-by":"publisher","first-page":"5620","DOI":"10.1038\/s41598-020-61724-6","article-title":"Preventive effect of fluvastatin on the development of medication-related osteonecrosis of the jaw","volume":"10","author":"Adachi","year":"2020","journal-title":"Sci Rep"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-020-03423-0","article-title":"Early imaging signs of the use of antiresorptive medication and MRONJ: a systematic review","volume":"24","author":"Moreno-Rabi\u00e9","year":"2020","journal-title":"Clin Oral Investigations"},{"key":"B32","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/S8756-3282(98)00068-4","article-title":"Morphometric analysis of human bone biopsies: A quantitative structural comparison of histological sections and micro-computed tomography","volume":"23","author":"M\u00fcller","year":"1998","journal-title":"Bone"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0214173","article-title":"Medication-related osteonecrosis of the jaws after tooth extraction in senescent female mice treated with zoledronic acid: Microtomographic, histological and immunohistochemical characterization","volume":"14","author":"Biguetti","year":"2019","journal-title":"PloS One"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2020.03.010","article-title":"Medication-related osteonecrosis of the jaws and quality of life: review and structured analysis","volume":"58","author":"Murphy","year":"2020","journal-title":"Br J Oral Maxillofac Surgery"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.1969.tb06001.x","article-title":"A study of the dimensional changes occurring in the maxilla following tooth extraction","volume":"14","author":"Johnson","year":"1969","journal-title":"Aust Dental J"},{"key":"B36","article-title":"Bone healing and soft tissue contour changes following single-tooth extraction: a clinical and radiographic 12-month prospective study","volume":"23","author":"Schropp","year":"2003","journal-title":"Int J Periodontics Restorative Dentistry"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2009.00878.x","article-title":"A novel animal model to study non-spontaneous bisphosphonates osteonecrosis of jaw","volume":"39","author":"Biasotto","year":"2010","journal-title":"J Oral Pathol Med"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.23","article-title":"Increased prevalence of bisphosphonate-related osteonecrosis of the jaw with vitamin D deficiency in rats","volume":"25","author":"Hokugo","year":"2010","journal-title":"J Bone Mineral Res"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12082","article-title":"Alveolar socket healing: what can we learn","volume":"68","author":"Ara\u00fajo","year":"2015","journal-title":"Periodontology 2000"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.08.015","article-title":"Broad-spectrum bactericidal activity of a new bioactive grafting material (F18) against clinically important bacterial strains","volume":"50","author":"Souza","year":"2017","journal-title":"Int J Antimicrobial Agents"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2020.1755842t","article-title":"Biomaterials for orthopedics: anti-biofilm activity of a new bioactive glass coating on titanium implants","volume":"36","author":"Marques","year":"2020","journal-title":"Biofouling"}],"container-title":["Frontiers in Oncology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2024.1348118\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T04:38:54Z","timestamp":1715315934000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fonc.2024.1348118\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,10]]},"references-count":41,"alternative-id":["10.3389\/fonc.2024.1348118"],"URL":"https:\/\/doi.org\/10.3389\/fonc.2024.1348118","relation":{},"ISSN":["2234-943X"],"issn-type":[{"value":"2234-943X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,10]]},"article-number":"1348118"}}