{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:34:10Z","timestamp":1791200050317,"version":"4.2.0"},"reference-count":16,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:00:00Z","timestamp":1706227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Cervical intraepithelial neoplasia grade 3 (CIN 3)-like SCC is a recently identified deceptive growth pattern that closely mimics endocervical crypt involvement by CIN 3. As CIN 3-like SCC is indistinguishable from endocervical crypt involvement by CIN 3, it poses a significant challenge for pathologists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>We examined 23 cases of CIN 3-like SCC, 6 of which also had concomitant conventional invasive SCC, and 9 cases of endocervical crypt involvement by CIN 3 as a control group.  Immunohistochemistry was used to investigate the expression of p16, E-cadherin, cyclin D1, and p53, and the expression of human papillomavirus (HPV) E6\/E7 mRNA, the key virus carcinogen of HPV, was detected. The clinicopathological, immunohistochemical, and molecular characteristics of endocervical crypt involvement by CIN 3, CIN 3-like SCC, and the concomitant conventional invasive SCC element were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>CIN 3-like SCC exhibited a characteristic morphology similar to endocervical crypt involvement by CIN 3, with pushing borders invading into the wall of the cervix, often to a significant depth in most cases. Immunophenotypic features of E-cadherin, p16, cyclin D1, and p53 differed between CIN 3-like SCC and conventional invasive SCC, both in staining intensity and region. E6\/E7 mRNA expression was higher in CIN 3-like SCC than in endocervical crypt involvement by CIN 3 (P &amp;lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CIN 3-like SCC is the type of cancer, presenting numerous challenges and potential for confusion as it mimics the phenotypes of endocervical crypt involvement by CIN 3.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fonc.2024.1354838","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T04:34:30Z","timestamp":1706243670000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Diagnostic validity of p16, E-cadherin, cyclin D1, p53, and HPV E6\/E7 mRNA in CIN 3-like squamous cell carcinoma of the cervix"],"prefix":"10.3389","volume":"14","author":[{"given":"Zhao","family":"Xing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Danhua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Xiaobo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1438.2000.00017.x","article-title":"Squamous carcinoma of the uterine cervix with CIN\u00a03-like pattern: an under-diagnosed lesion","volume":"10","author":"Al-Nafussi","year":"2000","journal-title":"Int J Gynecol Cancer"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1225652","article-title":"HPV prevalence and genotype\u00a0distribution in 2,306 patients with cervical squamous cell carcinoma in 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