{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:11:56Z","timestamp":1787508716488,"version":"build-2736575974"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873430, 81974414, 81772788, 82300199"],"award-info":[{"award-number":["81873430, 81974414, 81772788, 82300199"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Philadelphia-chromosome negative myeloproliferative neoplasms (MPN) exhibit phenotypic similarities with JAK\/STAT-unmutated idiopathic erythrocytosis and thrombocytosis (IE\/IT). We aimed to develop a clinical diagnostic model to discern MPN and IE\/IT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A retrospective study was performed on 77 MPN patients and 32 IE\/IT patients in our center from January 2018 to December 2023. We investigated the role of hemogram, cytokine and spleen size in differentiating MPN and IE\/IT among newly onset erythrocytosis and thrombocytosis patients. Independent influencing factors were integrated into a nomogram for individualized risk prediction. The calibration and discrimination ability of the model were evaluated by concordance index (C-index), calibration curve.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MPN had significantly higher TNF-\u03b1 level than IE\/IT, and the TNF-\u03b1 level is correlated with MF-grade. Multivariable analyses revealed that TNF-\u03b1, PLT count, age, size of spleen were independent diagnostic factors in differentiating MPN and IE\/IT. Nomograms integrated the above 4 factors for differentiating MPN and IE\/IT was internally validated and had good performance, the C-index of the model is 0.979.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The elevation of serum TNF-\u03b1 in MPN patients is of diagnostic significance and is correlated with the severity of myelofibrosis. The nomogram incorporating TNF-\u03b1 with age, PLT count and spleen size presents a noteworthy tool in the preliminary discrimination of MPN patients and those with idiopathic erythrocytosis or thrombocytosis. This highlights the potential of cytokines as biomarkers in hematologic disorders.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fonc.2024.1369346","type":"journal-article","created":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:51:48Z","timestamp":1711068708000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["TNF-\u03b1 is a predictive marker in distinguishing myeloproliferative neoplasm and idiopathic erythrocytosis\/thrombocytosis: development and validation of a non-invasive diagnostic 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