{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:23:38Z","timestamp":1761542618855,"version":"build-2065373602"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Artemisia absinthium<\/jats:italic>\n                      L. has a long history in the treatment of gastrointestinal cancer (GIC), but its molecular mechanisms remain unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      We identified and validated the active components and key targets in\n                      <jats:italic>A. absinthium<\/jats:italic>\n                      for the treatment of GIC by LC-MS and Network analysis. The antitumor effect of\n                      <jats:italic>A. absinthium<\/jats:italic>\n                      ethanol extract (AAEM-V) against gastrointestinal tract cancer\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      as well as the anticancer activity of the active ingredient, Luteolinidin, were further evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      AAEM-V exhibited good anticancer activity\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      . The active ingredient Luteolinidin was taken to intersect with the targets of gastric and colorectal cancers, and 69 common targets were identified. A total of three core targets, SRC, EGFR, and AKT1, were screened according to PPI and molecularly docked with Luteolinidin, and it was found that their binding played a key role in the treatment of tumors. Meanwhile, its active ingredient Luteolinidin was found to induce ROS proliferation to promote apoptosis and prevent cells from entering S phase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings not only explored the anti-gastrointestinal cancer chemical properties of\n                      <jats:italic>A. absinthium<\/jats:italic>\n                      , but also provided a new research direction for the active ingredients and mechanism of action of traditional Chinese medicine.\n                    <\/jats:p>\n                  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