{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T02:29:08Z","timestamp":1783477748194,"version":"3.55.0"},"reference-count":97,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:p>\n                    Head and neck squamous cell carcinoma (HNSCC) remains a prevalent epithelial malignancy. Immune-checkpoint inhibitors have reshaped first-line therapy for recurrent\/metastatic disease; yet durable benefit is confined to a subset, reflecting myeloid-centric mechanisms\u2014SPP1\n                    <jats:sup>+<\/jats:sup>\n                    TAM barriers, cDC1\/IL-12 insufficiency, and CXCL8\u2013CXCR1\/2\u2013driven neutrophil trafficking\u2014distinct from, and complementary to, classical lymphoid exhaustion. In this review we summarize advances from single-cell RNA and ATAC profiling and spatial transcriptomics that resolve macrophage, dendritic-cell and neutrophil programs, and appraise translational opportunities spanning myeloid reprogramming, innate\u2013adaptive combinations and spatial biomarkers. We also discuss enduring challenges\u2014including HPV-status heterogeneity, limited assay standardization and a scarcity of predictive metrics\u2014that temper implementation. By integrating myeloid-informed readouts (e.g., SPP1\u2013TAM burden, cDC1 competency, serum IL-8) with PD-1\u2013based regimens, EGFR-directed antibodies and myeloid checkpoints (CD47\u2013SIRP\u03b1, PI3K\u03b3, CXCR1\/2), emerging strategies aim to restore antigen presentation, improve lymphocyte trafficking and remodel tumor\u2013stroma interfaces. Our synthesis provides an appraisal of the evolving landscape of myeloid-informed precision immuno-oncology in HNSCC and outlines pragmatic standards and avenues for clinical translation. We hope these insights will assist researchers and clinicians as they endeavor to implement more effective, individualized regimens.\n                  <\/jats:p>","DOI":"10.3389\/fonc.2025.1693152","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T06:45:09Z","timestamp":1766040309000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Myeloid-driven immunosuppression in head and neck cancer: single-cell ATAC\/RNA and spatial transcriptomic perspectives"],"prefix":"10.3389","volume":"15","author":[{"given":"Rui","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University","place":["Chongqing, China"]},{"name":"Department of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, Chongqing Nan\u2019an District People\u2019s Hospital","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Chonggang General Hospital","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, The First Affiliated Hospital of Chongqing Medical University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,12,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"3349","DOI":"10.1038\/s41467-021-23355-x","article-title":"B cell 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