{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T05:03:26Z","timestamp":1785301406698,"version":"3.55.0"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:p>\n                    The data of scRNA-seq in specific databases were analyzed by bioinformatics. The expression level of miR-140-3p in osteosarcoma cells was detected using qRT-PCR, and the correlation between the expression level of miR-140-3p and the survival of tumor patients was analyzed using a database. A series of experiments were conducted to explore the role of miR-140-3p in osteosarcoma cells. Database prediction of target genes, dual luciferase reporter gene experiments, and a series of\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    experiments were used to explore the related mechanisms of miR-140-3p\u2019s influence on osteosarcoma proliferation and metastasis. MiR-140-3p was significantly lower expressed in osteosarcoma cells. Low expression of miR-140-3p is closely associated with poor survival of tumor patients.\n                    <jats:italic>In vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , overexpression of miR-140-3p can significantly inhibit the proliferation and metastasis of osteosarcoma cells, hinder the cycle process of osteosarcoma cells, and promote the apoptosis of osteosarcoma cells.\n                    <jats:italic>In vivo<\/jats:italic>\n                    , overexpression of miR-140-3p can significantly inhibit subcutaneous tumor formation and lung metastasis of osteosarcoma cells in nude mice. Knockdown of miR-140-3p has the opposite effect\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Mechanistically, UBE2C is a direct target gene of miR-140-3p, and UBE2C is highly expressed in osteosarcoma cells and most cancer tissues, and is associated with poor survival of tumor patients. The interaction between miR-140-3p and UBE2C can affect the activation of the PI3K\/Akt signaling pathway, which significantly changes the malignant biological characteristics of osteosarcoma cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The miR-140-3p\/UBE2C\/PI3K-Akt axis could be a promising target for treatment.\n                  <\/jats:p>","DOI":"10.3389\/fonc.2026.1742599","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:44:20Z","timestamp":1785300260000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["MiRNA-140-3p affects the proliferation and metastasis of osteosarcoma via the UBE2C\/PI3K-Akt axis"],"prefix":"10.3389","volume":"16","author":[{"given":"Xu","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Medicine, General Hospital of Northern Theater Command","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yubo","family":"Xiang","sequence":"additional","affiliation":[{"name":"Spinal Hospital of Enshi Tujia and Miao Autonomous Prefecture","place":["Enshi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoran","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The Forth Affiliated Hospital of China Medical University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengchen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, The Forth Affiliated Hospital of China Medical University","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Spinal Hospital of Enshi Tujia and Miao Autonomous Prefecture","place":["Enshi, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, General Hospital of Northern Theater Command","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, General Hospital of Northern Theater Command","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingxin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, General Hospital of Northern Theater Command","place":["Shenyang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq276","article-title":"Osteosarcoma","author":"Ritter","year":"2010","journal-title":"Ann Oncol"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24320","article-title":"International osteosarcoma incidence patterns in children and adolescents, middle ages and elderly persons","volume":"125","author":"Mirabello","year":"2009","journal-title":"Int J Cancer"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2013.11.006","article-title":"Osteosarcoma treatment - where do we stand? 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