{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:05:29Z","timestamp":1789707929783,"version":"4.0.1"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ferroptosis is increasingly implicated in endometrial cancer, but the role of the homologous recombination protein RAD51 in ferroptotic stress and its post-translational regulation remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RAD51 expression was assessed in GEO datasets, UCEC tissues, and endometrial cancer cell lines. RAD51 loss-of-function was evaluated using proliferation, cell-death, Transwell, ferroptosis-related, and xenograft assays. IP\u2013MS, co-immunoprecipitation, cycloheximide chase, proteasome inhibition, ubiquitination assays, USP26-C304S, and RAD51 rescue experiments were used to define USP26-dependent RAD51 regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RAD51 was upregulated in endometrial cancer and its depletion reduced proliferation and xenograft growth, lowered Transwell migration\/invasion readouts, and increased ferroptosis-associated changes. USP26 associated with RAD51, reduced its ubiquitination, and stabilized RAD51 in a catalytic activity-dependent manner. RAD51 re-expression partially rescued the effects of USP26 depletion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The USP26\u2013RAD51 axis supports endometrial cancer progression and limits ferroptosis-associated stress, identifying RAD51 protein stability as a potential mechanistic vulnerability.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fonc.2026.1896160","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:34:06Z","timestamp":1789706046000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["USP26-mediated stabilization of RAD51 promotes endometrial cancer progression and limits ferroptosis"],"prefix":"10.3389","volume":"16","author":[{"given":"Jiayu","family":"Jing","sequence":"first","affiliation":[{"name":"Department of Gynecology, Henan 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