{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:11:00Z","timestamp":1788952260966,"version":"build-2803163510"},"reference-count":17,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Oncol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Spinal glioblastoma (GBM) is an exceptionally rare entity and poses significant diagnostic challenges due to a substantial risk of neurological morbidity with surgical biopsy. Tissue-based analyses are frequently limited by sampling constraints and tumor heterogeneity. Conventional diagnostic modalities, including MRI and cerebrospinal fluid (CSF) cytology, often lack specificity and sensitivity. Commercially available CSF liquid biopsy platforms offer a minimally invasive approach for detecting tumor-derived cell-free DNA (cfDNA) to enable genomic profiling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We report a case of a 37-year-old male with spinal GBM who underwent prospective CSF cfDNA analysis using Belay Summit\u2122 and GTC Liquid Trace\u2122, yielding a provisional diagnosis of GBM and raising suspicion for Lynch syndrome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Subsequent tissue-based molecular profiling with Tempus\u2122 confirmed the diagnosis of GBM, while germline testing via Ambry Genetics\u2122 established Lynch syndrome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Based on the findings of mismatch repair deficiency and Lynch syndrome, the decision to proceed with immunotherapy was made. CSF cfDNA is a promising ancillary tool for guiding therapy in difficult-to-biopsy cases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fonc.2026.1914228","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:18:03Z","timestamp":1788949083000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report: Concordance of cerebrospinal fluid-derived circulating tumor DNA with tumor tissue in spinal glioblastoma associated with Lynch syndrome"],"prefix":"10.3389","volume":"16","author":[{"given":"Carlen A.","family":"Yuen","sequence":"first","affiliation":[{"name":"Department of Neurology, Division of Neuro-oncology, University of California, Irvine","place":["Irvine, CA, United States"]},{"name":"Chao Family Comprehensive Cancer Center, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David O.","family":"Kamson","sequence":"additional","affiliation":[{"name":"The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University","place":["Baltimore, MD, United States"]},{"name":"Department of Neurology, School of Medicine, Johns Hopkins University","place":["Baltimore, MD, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changrui","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Neurology, Division of Neuro-Genetics, Chao Family Comprehensive Cancer Center, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Pathology, Division of Neuropathology, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"Soun","sequence":"additional","affiliation":[{"name":"Department of Radiology, Division of Neuroradiology, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Linskey","sequence":"additional","affiliation":[{"name":"Chao Family Comprehensive Cancer Center, University of California, Irvine","place":["Irvine, CA, United States"]},{"name":"Department of Neurosurgery, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Paff","sequence":"additional","affiliation":[{"name":"Chao Family Comprehensive Cancer Center, University of California, Irvine","place":["Irvine, CA, United States"]},{"name":"Department of Neurosurgery, University of California, Irvine","place":["Irvine, CA, United 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CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansen","family":"Bow","sequence":"additional","affiliation":[{"name":"Chao Family Comprehensive Cancer Center, University of California, Irvine","place":["Irvine, CA, United States"]},{"name":"Department of Neurosurgery, University of California, Irvine","place":["Irvine, CA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/noab106","article-title":"The 2021 WHO classification of tumors of the central nervous system: a summary","volume":"23","author":"Louis","year":"2021","journal-title":"Neuro Oncol"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1200\/jco.2016.66.6552","article-title":"Immune checkpoint inhibition for hypermutant glioblastoma multiforme resulting from germline biallelic mismatch repair 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