{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T22:22:06Z","timestamp":1778019726535,"version":"3.51.4"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T00:00:00Z","timestamp":1688688000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Ophthalmol."],"abstract":"<jats:p>Our recent studies in mice suggest that a crucial event for the development of cataracts is the formation of calcium-containing deposits. To examine the generality of pathologic mineralization as a novel mechanism of cataract formation, we analyzed lens material from different human cataract surgeries. Human lens material was obtained from routine cataract surgeries performed on three patients with dense, white cataracts: a 10-month-old with congenital cataracts, a 9-year-old with a uveitic cataract, and a 17-year-old with a traumatic cataract. The aspirated material from the cataract surgeries contained insoluble material that could be isolated by centrifugation. Many particles within the insoluble fraction stained with Alizarin red, a dye that stains insoluble calcified material. The appearance of these human insoluble, Alizarin red-stained particles was similar to some of those detected in homogenates from cataractous mouse lenses. These results support the hypothesis that pathologic mineralization may have a mechanistic role in the formation of cataracts of different etiologies.<\/jats:p>","DOI":"10.3389\/fopht.2023.1213359","type":"journal-article","created":{"date-parts":[[2023,7,7]],"date-time":"2023-07-07T13:50:21Z","timestamp":1688737821000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Pediatric cataracts of different etiologies contain insoluble, calcified particles"],"prefix":"10.3389","volume":"3","author":[{"given":"Peter J.","family":"Minogue","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah H.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana M.","family":"Berthoud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric C.","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2016.156","article-title":"Global prevalence of childhood cataract: a systematic review","volume":"30","author":"Sheeladevi","year":"2016","journal-title":"Eye (Lond)"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.30135","article-title":"The prevalence of cataract in children","volume":"14","author":"Katre","year":"2022","journal-title":"Cureus"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2012.03.005","article-title":"Protein misfolding and aggregation in cataract disease and prospects for prevention","volume":"18","author":"Moreau","year":"2012","journal-title":"Trends Mol Med"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.19-27535","article-title":"Molecular processes implicated in human age-related nuclear cataract","volume":"60","author":"Truscott","year":"2019","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1002\/9780470720875.ch8","article-title":"Calcium and the physiology of cataract","volume":"106","author":"Duncan","year":"1984","journal-title":"Ciba Found Symp"},{"key":"B6","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1085\/jgp.200409121","article-title":"Connections between connexins, calcium, and cataracts in the lens","volume":"124","author":"Gao","year":"2004","journal-title":"J Gen Physiol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00277.2017","article-title":"Disruption of the lens circulation causes calcium accumulation and precipitates in connexin mutant mice","volume":"314","author":"Gao","year":"2018","journal-title":"Am J Physiol Cell Physiol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-26459","article-title":"The connexin50D47A mutant causes cataracts by calcium precipitation","volume":"60","author":"Berthoud","year":"2019","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B9","doi-asserted-by":"publisher","first-page":"17401","DOI":"10.1038\/s41598-021-96867-7","article-title":"Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis","volume":"11","author":"Li","year":"2021","journal-title":"Sci Rep"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.104935","article-title":"A crystallin mutant cataract with mineral deposits","author":"Minogue","year":"2023","journal-title":"J Biol Chem"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.951231","article-title":"Connexin mutants cause cataracts through deposition of apatite","volume":"10","author":"Minogue","year":"2022","journal-title":"Front Cell Dev Biol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1118\/1.2207135","article-title":"X-ray imaging in advanced studies of ophthalmic diseases","volume":"33","author":"Antunes","year":"2006","journal-title":"Med Phys"},{"key":"B13","first-page":"1","article-title":"Cataract. 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