{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T05:02:41Z","timestamp":1784350961502,"version":"3.55.0"},"reference-count":10,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>We aimed to quantify the DNA of maternal chimeric (MC) cells in the peripheral blood of the BA patients and investigated the impact on the outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with progressive jaundice because of no bile flow, which necessitated liver transplantation, or who showed inadequate bile flow with or without episodes of cholangitis and progressive hepatic fibrosis and portal hypertension were classified into the poor group. Those with adequate bile flow with completely normal liver function tests beyond 2 years were classified into the good group. The qPCR were separately carried out in buffy coat samples and plasma samples, targeting the non-inherited maternal HLA alleles in the DNA samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MC-DNA was present in the buffy coat (10\u2013328 gEq per 10<jats:sup>6<\/jats:sup> host cells) in seven patients. There was no MC-DNA in the remaining five patients. MC-DNA (214\u201315,331 gEq per 10<jats:sup>6<\/jats:sup> host cells) was observed in the plasma of five patients. The quantity of MC-DNA in the buffy coat showed a significant difference between the two prognostic groups (<jats:italic>p<\/jats:italic> = 0.018), whereas there was no significant difference in the quantity of MC-DNA in plasma (<jats:italic>p<\/jats:italic> = 0.205). MC-DNA in the buffy coat was significantly associated with the outcome (<jats:italic>p<\/jats:italic> = 0.028), whereas MC-DNA in the plasma did not influence the outcome (<jats:italic>p<\/jats:italic> = 0.56).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Poor outcomes in BA were correlated with circulating maternal chimeric lymphocytes.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fped.2022.1007927","type":"journal-article","created":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T07:31:51Z","timestamp":1663659111000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Circulating maternal chimeric cells have an impact on the outcome of biliary atresia"],"prefix":"10.3389","volume":"10","author":[{"given":"Ryuta","family":"Masuya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Muraji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami B.","family":"Kanaan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Harumatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Muto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miki","family":"Toma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Yanai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Stevens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Lee","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Nakame","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Nanashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Ieiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/1471-230X-4-14","article-title":"Maternal microchimerism in the livers of patients with biliary atresia","volume":"4","author":"Suskind","year":"2020","journal-title":"BMC Gastroenterol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1016\/j.jpedsurg.2007.01.051","article-title":"Maternal microchimerism in biliary atresia","volume":"42","author":"Kobayashi","year":"2007","journal-title":"J Pediatr Surg."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2097","DOI":"10.1016\/j.jpedsurg.2007.08.039","article-title":"The evidence of maternal microchimerism in biliary atresia using fluorescent in situ hybridization","volume":"42","author":"Hayashida","year":"2007","journal-title":"J Pediatr Surg."},{"key":"B4","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1542\/peds.2007-0568","article-title":"Maternal microchimerism in underlying pathogenesis of biliary atresia: quantification and phenotypes of maternal cells in the liver","volume":"121","author":"Muraji","year":"2008","journal-title":"Pediatrics."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1038\/s41409-020-01149-x","article-title":"Cord blood maternal microchimerism following unrelated cord blood transplantation","volume":"56","author":"Kanaan","year":"2021","journal-title":"Bone Marrow Transplant."},{"key":"B6","doi-asserted-by":"publisher","first-page":"59","DOI":"10.4161\/chim.29844","article-title":"Feto-maternal allo-immunity, regulatory T cells and predisposition to auto-immunity: does it all start in utero?","volume":"5","author":"Leveque","year":"2014","journal-title":"Chimerism."},{"key":"B7","doi-asserted-by":"publisher","first-page":"1249","DOI":"10.1007\/s00383-013-3421-6","article-title":"Elevated Th17 cells accompanied by decreased regulatory T cells and cytokine environment in infants with biliary atresia","volume":"29","author":"Yang","year":"2013","journal-title":"Pediatr Surg Int"},{"key":"B8","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1002\/hep4.1332","article-title":"Correlation of immune markers with outcomes in biliary atresia following intravenous immunoglobulin therapy","volume":"3","author":"Kim","year":"2019","journal-title":"Hepatol Commun."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e83","DOI":"10.1097\/MPG.0000000000003388","article-title":"Quantification of maternal microchimeric cells in the liver of children with biliary atresia","volume":"74","author":"Tamaoka","year":"2022","journal-title":"J Pediatr Gastroenterol Nutr."},{"key":"B10","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1097\/MPG.0000000000002929","article-title":"Segmental atrophy of explanted livers in biliary atresia: pathological data from 63 cases of failed portoenterostomy","volume":"72","author":"Santo","year":"2021","journal-title":"J Pediatr Gastoenterol Nutr."}],"container-title":["Frontiers in Pediatrics"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fped.2022.1007927\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T07:31:53Z","timestamp":1663659113000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fped.2022.1007927\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,20]]},"references-count":10,"alternative-id":["10.3389\/fped.2022.1007927"],"URL":"https:\/\/doi.org\/10.3389\/fped.2022.1007927","relation":{},"ISSN":["2296-2360"],"issn-type":[{"value":"2296-2360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,20]]},"article-number":"1007927"}}