{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:51:35Z","timestamp":1754398295626,"version":"3.41.2"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T00:00:00Z","timestamp":1654473600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To assess the effects of cannabidiol (CBD) on lung damage in a piglet model of meconium aspiration syndrome (MAS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Meconium aspiration syndrome was modelled in newborn piglets <jats:italic>via<\/jats:italic> intratracheal instillation of 20% meconium in saline collected from healthy newborn humans. Piglets were treated i.v. with 5 mg\/kg CBD (MAS + CBD) or Vehicle (MAS + VEH) 30 min after MAS induction and monitored for 6 h. Ventilated piglets without meconium instillation served as controls (CTL). Ventilatory and haemodynamic monitoring, histological and biochemical studies assessed the effects of treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Post-insult administration of CBD reduced MAS-induced deterioration of gas exchange, improving respiratory acidosis (final pH 7.38 \u00b1 0.02, 7.22 \u00b1 0.03 and 7.33 \u00b1 0.03 and final pCO<jats:sub>2<\/jats:sub> 39.8 \u00b1 1.3, 60.4 \u00b1 3.8 and 45.7 \u00b1 3.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, <jats:italic>p<\/jats:italic> &amp;lt; 0.05). These beneficial effects were obtained despite the less aggressive ventilatory settings required for CBD-treated animals (final minute volume 230 \u00b1 30, 348 \u00b1 33 and 253 \u00b1 24 mL\/kg\/min and final Oxygenation Index 1.64 \u00b1 0.04, 12.57 \u00b1 3.10 and 7.42 \u00b1 2.07 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, <jats:italic>p<\/jats:italic> &amp;lt; 0.05). CBD\u2019s beneficial effects on gas exchange were associated with reduced histological lung damage, reduced leucocyte infiltration and oedema (histopathological score 1.6 \u00b1 0.3, 8.6 \u00b1 1.4 and 4.6 \u00b1 0.7 points for CTL, MAS + VEH and MAS + CBD, respectively, <jats:italic>p<\/jats:italic> &amp;lt; 0.05), as well as reduced TNF\u03b1 production (0.04 \u00b1 0.01, 0.34 \u00b1 0.06 and 0.12 \u00b1 0.02 A.U. for CTL, MAS + VEH and MAS + CBD, respectively, <jats:italic>p<\/jats:italic> &amp;lt; 0.05). Moreover, CBD improved blood pressure stability (final mean blood pressure 74.5 \u00b1 0.2, 62.2 \u00b1 6.2, and 78.67 \u00b1 4.1 mmHg for CTL, MAS + VEH and MAS + CBD, respectively, <jats:italic>p<\/jats:italic> &amp;lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cannabidiol reduces histologic lung damage and inflammation in a piglet model of MAS. This translates into improved gas exchange and blood pressure stability.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fped.2022.862035","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T07:03:35Z","timestamp":1654499015000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Cannabidiol Reduces Inflammatory Lung Damage After Meconium Aspiration in Newborn Piglets"],"prefix":"10.3389","volume":"10","author":[{"given":"Luis","family":"Arruza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Barata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Vierge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria 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