{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T21:55:32Z","timestamp":1772747732668,"version":"3.50.1"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T00:00:00Z","timestamp":1658966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The early diagnosis of tuberculous pleural effusion (TPE) is challenging due to the difficulty of isolating<jats:italic>Mycobacterium tuberculosis<\/jats:italic>, and pleural biomarkers are an optional choice. Recent studies showed that interleukin-27 (IL-27) appears to be a new accurate biomarker for TPE in adults and no related studies were reported in children. In this study, we aimed to evaluate the potential value of IL-27 in pediatric tuberculous pleurisy by detecting its levels in pleural fluid and serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 48 children with TPE and 64 children with severe<jats:italic>Mycoplasma pneumoniae<\/jats:italic>(MP) pneumonic effusion (SMPPE) were enrolled in this study. IL-27 concentrations were measured in serum and pleural fluid. The diagnostic yield of IL-27 was evaluated using receiver operating characteristic (ROC) curves.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The level of p-IL-27 in TPE showed statistically no significant difference when compared with SMPPE (<jats:italic>p<\/jats:italic>&amp;gt; 0.05). However, pleural fluid IL-27 (p-IL-27) \/ serum IL-27 (s-IL-27) ratio in TPE were significantly much higher than those in SMPPE (<jats:italic>p<\/jats:italic>&amp;lt; 0.05). By the analysis of the ROC curves, the diagnostic sensitivity and specificity of the p-IL-27\/s-IL-27 ratio were 100% and 48.44%, respectively (cutoff value of 1.0280). The area under the ROC curve for p-IL-27\/s-IL-27 was 0.7295.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Pleural fluid IL-27 alone was not accurate in distinguishing pediatric TPE from SMPPE, which was different from the diagnostic value of IL-27 in adult studies due to the different disease spectra between children and adults. Our results implied that the p-IL-27\/s-IL-27 ratio had a potential value in distinguishing TPE from SMPPE. However, the specificity of IL-27 was relatively lower and it is necessary to find a more specific marker in tuberculous pleurisy of children.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fped.2022.948862","type":"journal-article","created":{"date-parts":[[2022,7,28]],"date-time":"2022-07-28T11:31:22Z","timestamp":1659007882000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["The value of interleukin-27 for differentiating tuberculous pleural effusion from Mycoplasma pneumoniae pneumonic effusion in children"],"prefix":"10.3389","volume":"10","author":[{"given":"Hui","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiming","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siyu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunying","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,28]]},"reference":[{"key":"B1","year":"2020","journal-title":"Global Tuberculosis Report 2020 Licence: CC BY-NC-SA 3.0 IGO."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1090","DOI":"10.1080\/22221751.2022.2054367","article-title":"Epidemiology of extrapulmonary tuberculosis among pediatric inpatients in mainland China: a descriptive, multicenter study.","volume":"11","author":"Chu","year":"2022","journal-title":"Emerg Microbes Infect."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1190","DOI":"10.1001\/archinte.150.6.1190","article-title":"Cytologic and bacteriologic analysis of fluid and pleural biopsy specimens with Cope\u2019s needle. 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