{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:23:42Z","timestamp":1761157422565,"version":"3.41.2"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The etiology and underlying pathogenic mechanisms of Legg-Calve-Perthes disease (LCPD) still remain unclear. A disruption of blood supply to the femoral head, producing ischemic necrosis, appears to be the critical pathological event. The lncRNAs play crucial roles in many biological processes and are dysregulated in various human diseases. However, its expression profiles and the potential regulatory roles in the development of LCPD have not been investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, differentially expressed lncRNA and mRNA of Legg-Calve-Perthes disease patients were profiled. Several GO terms and pathways that play important roles in the regulation of vascular structure, function or coagulation were selected for further analysis. The lncRNA -mRNA interacting networks in LCPD tissues were constructed to identify novel potential targets for further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The microarray analysis revealed that 149 lncRNAs and 37 mRNAs were up-regulated, and 64 lncRNAs and 250 mRNAs were down-regulated in LCPD tissues. After filtering, we finally found 14 mRNAs and constructed an mRNA-lncRNA interacting network. Through the analysis of the interaction network, we finally found 13 differentially expressed lncRNAs, which may be implicated in the pathogenesis of LCPD. These mRNAs\/lncRNAs were further validated with qRT-PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings of this study established a co-expression network of disease-related lncRNAs and mRNAs which screened out from the concerned G.O. terms and Pathways, which may provide new sights for future studies on molecular mechanisms of LCPD.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fped.2022.974547","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T06:27:27Z","timestamp":1662532047000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Microarray analysis of lncRNA and mRNA expression profiles in patients with Legg-Calve-Perthes disease"],"prefix":"10.3389","volume":"10","author":[{"given":"Shangyu","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haobo","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renhao","family":"Ze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pan","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"B1","first-page":"86","article-title":"Evolution in diagnosis and treatment of Legg-Calve-Perthes disease","volume":"2","author":"Mazloumi","year":"2014","journal-title":"Arch Bone Jt Surg."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e4","DOI":"10.2106\/JBJS.RVW.15.00063","article-title":"The pathogenesis and treatment of Legg-Calv\u00e9-Perthes disease","volume":"4","author":"Ibrahim","year":"2016","journal-title":"JBJS Rev."},{"key":"B3","doi-asserted-by":"publisher","first-page":"S141","DOI":"10.1097\/BPO.0b013e318223b4bd","article-title":"Legg-Calve-Perthes disease: etiology, pathogenesis, and biology","volume":"31","author":"Kim","year":"2011","journal-title":"J Pediatr Orthop."},{"key":"B4","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1302\/0301-620X.82B3.0820392","article-title":"The blood supply of the lateral epiphyseal arteries in Perthes' disease","volume":"82","author":"Atsumi","year":"2000","journal-title":"J Bone Joint Surg Br."},{"key":"B5","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1097\/00003086-198412000-00028","article-title":"Angiography in Perthes' disease","volume":"191","author":"de Camargo","year":"1984","journal-title":"Clin Orthop Relat Res"},{"key":"B6","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1148\/radiology.135.1.7360984","article-title":"Angiography in Legg-Calv\u00e9-Perthes disease","volume":"135","author":"Th\u00e9ron","year":"1980","journal-title":"Radiology."},{"key":"B7","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1016\/S0001-2998(05)80109-6","article-title":"A scintigraphic classification of Legg-Calv\u00e9-Perthes disease","volume":"23","author":"Conway","year":"1993","journal-title":"Semin Nucl Med."},{"key":"B8","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1007\/s00247-002-0732-5","article-title":"Femoral head vascularisation in Legg-Calv\u00e9-Perthes disease: comparison of dynamic gadolinium-enhanced subtraction MRI with bone scintigraphy","volume":"32","author":"Lamer","year":"2002","journal-title":"Pediatr Radiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1329","DOI":"10.2106\/00004623-200208000-00007","article-title":"Development of flattening and apparent fragmentation following ischemic necrosis of the capital femoral epiphysis in a piglet model","volume":"84","author":"Kim","year":"2002","journal-title":"J Bone Joint Surg Am."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2903","DOI":"10.2106\/JBJS.H.01464","article-title":"Femoral head deformation and repair following induction of ischemic necrosis: a histologic and magnetic resonance imaging study in the piglet","volume":"91","author":"Shapiro","year":"2009","journal-title":"J Bone Joint Surg Am."},{"key":"B11","doi-asserted-by":"publisher","first-page":"526","DOI":"10.3109\/17453678408992952","article-title":"Effects of vascular occlusion on the femoral head in growing rabbits","volume":"55","author":"Calvert","year":"1984","journal-title":"Acta Orthop Scand."},{"key":"B12","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1179\/102453308X343509","article-title":"Thrombomodulin and GFC levels in Legg-Calve-Perthes disease","volume":"13","author":"Aksoy","year":"2008","journal-title":"Hematology."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2642","DOI":"10.2106\/00004623-200412000-00009","article-title":"Legg-Calve-Perthes disease and thrombophilia","volume":"86","author":"Balasa","year":"2004","journal-title":"J Bone Joint Surg Am."},{"key":"B14","doi-asserted-by":"publisher","first-page":"121","DOI":"10.2106\/JBJS.I.00157","article-title":"Coagulation abnormalities in Legg-Calv\u00e9-Perthes disease","volume":"92","author":"Vosmaer","year":"2010","journal-title":"J Bone Joint Surg Am."},{"key":"B15","doi-asserted-by":"publisher","first-page":"e126","DOI":"10.1542\/peds.2011-3269","article-title":"Abnormalities of vascular structure and function in children with Perthes disease","volume":"130","author":"Perry","year":"2012","journal-title":"Pediatrics."},{"key":"B16","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1186\/s12891-015-0730-z","article-title":"Comparative study of serum proteomes in Legg-Calve-Perthes disease","volume":"16","author":"Liu","year":"2015","journal-title":"BMC Musculoskelet Disord."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1913","DOI":"10.3892\/etm.2016.3111","article-title":"Endothelial nitric oxide synthase gene polymorphism is associated with Legg-Calv\u00e9-Perthes disease","volume":"11","author":"Zhao","year":"2016","journal-title":"Exp Ther Med."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e100505","DOI":"10.1371\/journal.pone.0100505","article-title":"A novel p. Gly630Ser mutation of COL2A1 in a Chinese family with presentations of Legg-Calv\u00e9-Perthes disease or avascular necrosis of the femoral head","volume":"9","author":"Li","year":"2014","journal-title":"PLoS ONE."},{"key":"B19","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3109\/17453679209154752","article-title":"Low plasma levels of insulin-like growth factor I in Perthes' disease. A controlled study of 59 consecutive children","volume":"63","author":"Neidel","year":"1992","journal-title":"Acta Orthop Scand."},{"key":"B20","doi-asserted-by":"publisher","first-page":"e410","DOI":"10.1097\/MPH.0000000000000203","article-title":"Increased soluble selectins as a reflection of activated platelets and endothelium in Legg-Calve-Perthes disease","volume":"36","author":"Ismayilov","year":"2014","journal-title":"J Pediatr Hematol Oncol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1002\/jor.22415","article-title":"Role of leptin in Legg-Calv\u00e9-Perthes disease","volume":"31","author":"Lee","year":"2013","journal-title":"J Orthop Res."},{"key":"B22","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.bbagrm.2015.06.015","article-title":"lncRNAs and microRNAs with a role in cancer development","volume":"1859","author":"Liz","year":"2016","journal-title":"Biochim Biophys Acta."},{"key":"B23","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1038\/nrg3074","article-title":"Non-coding RNAs in human disease","volume":"12","author":"Esteller","year":"2011","journal-title":"Nat Rev Genet."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.yexcr.2017.10.010","article-title":"Translation of noncoding RNAs: Focus on lncRNAs, pri-miRNAs, and circRNAs","volume":"361","author":"Li","year":"2017","journal-title":"Exp Cell Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1016\/j.stem.2014.05.014","article-title":"Long noncoding RNAs in cell-fate programming and reprogramming","volume":"14","author":"Flynn","year":"2014","journal-title":"Cell Stem Cell."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1932","DOI":"10.1002\/cmdc.201300534","article-title":"Long noncoding RNAs: emerging stars in gene regulation, epigenetics and human disease","volume":"9","author":"Bhan","year":"2014","journal-title":"ChemMedChem."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.mrrev.2014.04.002","article-title":"lncRNAs: insights into their function and mechanics in underlying disorders","volume":"762","author":"Li","year":"2014","journal-title":"Mutat Res Rev Mutat Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1015","DOI":"10.3390\/cells8091015","article-title":"Long non-coding RNA in the pathogenesis of cancers","volume":"8","author":"Chi","year":"2019","journal-title":"Cells."},{"key":"B29","doi-asserted-by":"publisher","first-page":"9127","DOI":"10.1038\/s41598-018-27501-2","article-title":"LncRNA expression profiling of BMSCs in osteonecrosis of the femoral head associated with increased adipogenic and decreased osteogenic differentiation","volume":"8","author":"Wang","year":"2018","journal-title":"Sci Rep."},{"key":"B30","doi-asserted-by":"publisher","first-page":"18435","DOI":"10.1002\/jcb.29161","article-title":"The role of lncRNA RP11-154D6 in steroid-induced osteonecrosis of the femoral head through BMSC regulation","volume":"120","author":"Xiang","year":"2019","journal-title":"J Cell Biochem."},{"key":"B31","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1161\/CIRCRESAHA.111.253948","article-title":"Integrin-linked kinase regulates vasomotor function by preventing endothelial nitric oxide synthase uncoupling: role in atherosclerosis","volume":"110","author":"Herranz","year":"2012","journal-title":"Circ Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1161\/ATVBAHA.117.309560","article-title":"iNOS-derived nitric oxide induces integrin-linked kinase endocytic lysosome-mediated degradation in the vascular endothelium","volume":"37","author":"Reventun","year":"2017","journal-title":"Arterioscler Thromb Vasc Biol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"2087","DOI":"10.1002\/jbmr.3190","article-title":"Integrin-linked kinase regulates bone formation by controlling cytoskeletal organization and modulating BMP and Wnt signaling in osteoprogenitors","volume":"32","author":"Dejaeger","year":"2017","journal-title":"J Bone Miner Res."},{"key":"B34","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1177\/1534734616649485","article-title":"Integrin-Linked Kinase Senses Hypoxia During Scar Angiogenesis","volume":"15","author":"Li","year":"2016","journal-title":"Int J Low Extrem Wounds."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2999","DOI":"10.1007\/s00018-017-2511-3","article-title":"Vinculin in cell-cell and cell-matrix adhesions","volume":"74","author":"Bays","year":"2017","journal-title":"Cell Mol Life Sci."},{"key":"B36","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s00018-016-2325-8","article-title":"Cell-cell junctional mechanotransduction in endothelial remodeling","volume":"74","author":"Dorland","year":"2017","journal-title":"Cell Mol Life Sci."},{"key":"B37","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1159\/000444526","article-title":"R-Ras Inhibits VEGF-Induced p38MAPK Activation and HSP27 Phosphorylation in Endothelial Cells","volume":"52","author":"Sawada","year":"2015","journal-title":"J Vasc Res."},{"key":"B38","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s13023-022-02275-z","article-title":"Legg-Calv\u00e9-Perthes disease overview","volume":"17","author":"Rodr\u00edguez-Olivas","year":"2022","journal-title":"Orphanet J Rare Dis."}],"container-title":["Frontiers in Pediatrics"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fped.2022.974547\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T06:27:32Z","timestamp":1662532052000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fped.2022.974547\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,7]]},"references-count":38,"alternative-id":["10.3389\/fped.2022.974547"],"URL":"https:\/\/doi.org\/10.3389\/fped.2022.974547","relation":{},"ISSN":["2296-2360"],"issn-type":[{"type":"electronic","value":"2296-2360"}],"subject":[],"published":{"date-parts":[[2022,9,7]]},"article-number":"974547"}}