{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T22:06:48Z","timestamp":1781561208483,"version":"3.54.5"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Hirschsprung Disease (HSCR) is a common congenital intestinal disease in pediatrics. Early diagnosis and treatment after birth alleviate the occurrence of complications. Consequently, we aim to identifiy a biomarker with ease of use, non-invasiveness, and highly accurate for diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma samples were collected from HSCR group, other intestinal disease controls (DC) and healthy controls (HC)<jats:bold>,<\/jats:bold> while colon samples were collected from HSCR and DC groups. We conducted human neural autoantibody microarray analyses on plasma. The candidate biomarker was further validated using enzyme-linked immunosorbent assay (ELISA) in colon tissue and plasma. The receiver operating characteristic curve (ROC) was used to assess the diagnostic performance of the plasma biomarker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Microarray analysis revealed that the level of plasma N-methyl-D-Aspartate receptor (NMDAR) autoantibody in HSCR group was significantly higher than those in the HC group (<jats:italic>p<\/jats:italic>\u2009=\u20090.008). In plasma analyzed cohort, the level of NMDAR autoantibodies in HSCR group (<jats:italic>n<\/jats:italic>\u2009=\u200938) were significantly elevated compared to both the HC (<jats:italic>n<\/jats:italic>\u2009=\u200931, <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.0001) and the DC (<jats:italic>n<\/jats:italic>\u2009=\u200920, <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.0001). We further validated the diagnostic efficacy of plasma NMDAR autoantibody, it demonstrated AUCs of 0.96 and 0.81 for diagnosing HSCR when compared to HC and DC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Plasma NMDAR autoantibody might be served as an efficient, non-invasive biomarker for diagnosing HSCR.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fped.2025.1514323","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T06:39:31Z","timestamp":1740119971000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Plasma NMDAR autoantibody: a new biomarker for the diagnosis of Hirschsprung 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