{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:31:17Z","timestamp":1780381877930,"version":"3.54.1"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["\u7f16\u53f7\uff1a2022A1515010427"],"award-info":[{"award-number":["\u7f16\u53f7\uff1a2022A1515010427"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:p>\n                    In the neonatal period, infectious diseases associated with high morbidity (e.g., neonatal sepsis and meningitis) are preliminarily assessed using indicators like C-reactive protein (CRP) and procalcitonin, but definitive diagnosis relies on pathogen detection through methods such as blood culture, which is time-consuming and has low sensitivity. To improve diagnostic efficiency, metagenomic next-generation sequencing (mNGS) is increasingly utilized, offering three testing modalities: DNA-only, RNA-only, and combined DNA+RNA channels. This retrospective study analyzed 894 clinical samples (peripheral blood, sputum, bronchoalveolar lavage fluid) to compare detection rates across channels. The overall mNGS positivity rate was 51.9% (464\/894), with no significant differences among DNA-only (50.8%), RNA-only (55.7%), and combined channels (49.6%) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&amp;gt;\u20090.05). Notably, bronchoalveolar lavage fluid samples exhibited the highest positivity rate (84.57%, 148\/175), reaching 97.33% (73\/75) with dual-channel testing. Sputum samples showed a 53.7% positivity rate (87\/162), increasing to 82.35% (14\/17) with dual-channel detection. Conversely, peripheral blood had an overall positivity rate of 43.14% (132\/306), with the DNA-only channel outperforming RNA-only and dual channels (45.34% 5s. 43.00% and 34.21%). These findings underscore the importance of channel selection based on sample type to optimize diagnostic accuracy and cost-effectiveness.\n                  <\/jats:p>","DOI":"10.3389\/fped.2025.1632123","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T06:30:50Z","timestamp":1763706650000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Channel selection of metagenomic next-generation sequencing in infants pathogen detection: a multicenter cross-sectional study"],"prefix":"10.3389","volume":"13","author":[{"given":"Chengcheng","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Neonatology, Nanfang Hospital, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minxu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Dongguan Women and Children Hospital","place":["Dongguan, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children Hospital","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianer","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Jiangmen Women and Children Hospital","place":["Jiangmen, Guangdong, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Nanfang Hospital, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neonatology, Guangdong Women and Children Hospital","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"9800","DOI":"10.1080\/14767058.2022.2054320","article-title":"Prevalence of major morbidities and outcome of all hospitalized neonates. 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