{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:33Z","timestamp":1788419793114,"version":"build-2803163510"},"reference-count":23,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pediatr."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Endothelial activation is implicated in childhood IgA vasculitis, but candidate endothelial markers may reflect systemic inflammation rather than endothelial perturbation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this single-centre cross-sectional pilot study, we enrolled 13 children with acute IgA vasculitis and 20 age- and sex-comparable healthy controls screened to exclude acute infection, allergic disease and other inflammatory conditions. Plasma soluble fms-like tyrosine kinase 1, soluble vascular endothelial cadherin, von Willebrand factor (vWF) and vascular endothelial growth factor were measured by enzyme-linked immunosorbent assay (ELISA); CD45-low CD31\n                      <jats:sup>+<\/jats:sup>\n                      CD146\n                      <jats:sup>+<\/jats:sup>\n                      endothelial-lineage events were quantified by three-colour flow cytometry in a subset.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Children with IgA vasculitis had higher C-reactive protein, neutrophil proportion and platelet count than controls. In the pooled analysis, vWF was higher in patients than controls (median 811.1 vs. 439.9 ng\/mL; Hodges-Lehmann difference 335 ng\/mL, 95% confidence interval 133-539;\n                      <jats:italic>P<\/jats:italic>\n                      = 0.008; Benjamini-Hochberg\n                      <jats:italic>q<\/jats:italic>\n                      = 0.03), but this contrast did not persist after ELISA plate stratification (van Elteren\n                      <jats:italic>P<\/jats:italic>\n                      = 0.38) and is therefore hypothesis-generating. Within patients, no clear monotonic associations were detected between vWF and C-reactive protein or neutrophil indices; these exploratory analyses were underpowered to establish independence from systemic inflammation. No clear group differences were detected in the other soluble analytes. Endothelial-lineage events were nominally higher in patients, but this readout was strongly affected by CD45-low gate size and acquisition-session variability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These findings support batch-controlled validation of vWF and more rigorous endothelial-cell enumeration in future pediatric IgA vasculitis studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fped.2026.1930009","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:42:47Z","timestamp":1788417767000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Plasma von Willebrand factor and endothelial-lineage flow-cytometry signals in acute pediatric IgA vasculitis: a cross-sectional pilot study"],"prefix":"10.3389","volume":"14","author":[{"given":"Zichao","family":"Ding","sequence":"first","affiliation":[{"name":"Pediatric Hospital of the First Affiliated Hospital of Henan University of Chinese Medicine","place":["Zhengzhou, China"]},{"name":"College of Pediatrics, Henan University of Chinese Medicine","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianqing","family":"Ren","sequence":"additional","affiliation":[{"name":"Pediatric Hospital of the First Affiliated Hospital of Henan University of Chinese Medicine","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Su","sequence":"additional","affiliation":[{"name":"Pediatric Hospital of the First Affiliated Hospital of Henan University of Chinese Medicine","place":["Zhengzhou, 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