{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:09:10Z","timestamp":1792595350428,"version":"4.5.0"},"reference-count":49,"publisher":"Frontiers Media SA","content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"DOI":"10.3389\/fphar.2016.00363","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T03:36:29Z","timestamp":1476329789000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":28,"title":["The Droplet Digital PCR: A New Valid Molecular Approach for the Assessment of B-RAF V600E Mutation in Hairy Cell Leukemia"],"prefix":"10.3389","volume":"7","author":[{"given":"Francesca","family":"Guerrini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Paolicchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Ghio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ciabatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Grassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Salehzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacomo","family":"Ercolano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria R.","family":"Metelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzia","family":"Del Re","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Iovino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iacopo","family":"Petrini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Carulli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Cecconi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Rousseau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Cervetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Galimberti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2016,10,13]]},"reference":[{"issue":"253","key":"B1","doi-asserted-by":"publisher","DOI":"10.4081\/oncol.2014.253","article-title":"BRAF mutation in hairy cell leukemia.","volume":"8","author":"Ahmadzadeh","year":"2014","journal-title":"Oncol. Rev."},{"key":"B2","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1182\/blood-2011-08-368209","article-title":"The BRAF V600E mutation in hairy cell leukemia and other mature B-cell neoplasms.","volume":"119","author":"Arcaini","year":"2012","journal-title":"Blood"},{"key":"B3","doi-asserted-by":"publisher","first-page":"2804","DOI":"10.1158\/1078-0432.CCR-05-2315","article-title":"Minimal residual disease in hairy cell leukemia patients assessed by clone-specific polymerase chain reaction.","volume":"12","author":"Arons","year":"2006","journal-title":"Clin. Cancer Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1182\/blood-2013-05-501569","article-title":"European LeukemiaNet recommendations for the management of chronic myeloid leukemia: 2013.","volume":"122","author":"Baccarani","year":"2013","journal-title":"Blood"},{"key":"B5","doi-asserted-by":"publisher","first-page":"e157","DOI":"10.3324\/haematol.2015.135467","article-title":"Droplet digital polymerase chain reaction for DNMT3A and IDH1\/2 mutations to improve early detection of acute myeloid leukemia relapse after allogeneic hematopoietic stem cell transplantation.","volume":"101","author":"Brambati","year":"2016","journal-title":"Haematologica"},{"key":"B6","doi-asserted-by":"publisher","first-page":"6313","DOI":"10.1158\/1078-0432.CCR-13-1848","article-title":"Bendamustine and rituximab in relapsed and refractory hairy cell leukemia.","volume":"19","author":"Burotto","year":"2013","journal-title":"Clin. Cancer Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/0161-5890(92)90100-C","article-title":"Use of family specific leader region primers for PCR amplification of human heavy chain variable region gene repertoire.","volume":"29","author":"Campbell","year":"1992","journal-title":"Mol. Immunol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"5019","DOI":"10.1182\/blood-2011-01-293050","article-title":"The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications.","volume":"117","author":"Campo","year":"2011","journal-title":"Blood"},{"key":"B9","doi-asserted-by":"publisher","first-page":"2171","DOI":"10.3109\/10428194.2016.1139703","article-title":"Digital PCR for quantification of recurrent and potentially actionable somatic mutations in circulating free DNA from patients with diffuse large B-cell lymphoma.","volume":"57","author":"Camus","year":"2016","journal-title":"Leuk. Lymphoma"},{"key":"B10","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.critrevonc.2016.02.012","article-title":"BRAF mutations in non-small cell lung cancer: has finally Janus opened the door?","volume":"101","author":"Caparica","year":"2016","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1111\/j.1600-0609.2004.00325.x","article-title":"Rituximab as treatment for minimal residual disease in hairy cell leukaemia.","volume":"73","author":"Cervetti","year":"2004","journal-title":"Eur. J. Haematol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1111\/j.1365-2141.2008.07333.x","article-title":"Rituximab as treatment for minimal residual disease in hairy cell leukaemia: extended follow-up.","volume":"143","author":"Cervetti","year":"2008","journal-title":"Br. J. Haematol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/1120009X.2015.1114222","article-title":"How to treat splenic marginal zone lymphoma (SMZL) in patients unfit for surgery or more aggressive therapies: experience in 30 cases.","volume":"25","author":"Cervetti","year":"2016","journal-title":"J. Chemother."},{"key":"B14","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/S0898-6568(02)00139-0","article-title":"Mechanisms of regulating the Raf kinase family.","volume":"15","author":"Chong","year":"2003","journal-title":"Cell. Signal."},{"key":"B15","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1038\/nature00766","article-title":"Mutations of the BRAF gene in human cancer.","volume":"417","author":"Davies","year":"2002","journal-title":"Nature"},{"key":"B16","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1002\/hon.2095","article-title":"Comparison of two real-time quantitative polymerase chain reaction strategies for minimal residual disease evaluation in lymphoproliferative disorders: correlation between immunoglobulin gene mutation load and real-time quantitative polymerase chain reaction performance.","volume":"32","author":"Della Starza","year":"2014","journal-title":"Hematol. Oncol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1002\/hon.2095","article-title":"Comparative analysis between RQ-PCR and digital-droplet-PCR of immunoglobulin\/T-cell receptor gene rearrangements to monitor minimal residual disease in acute lymphoblastic leukaemia.","volume":"174","author":"Della Starza","year":"2016","journal-title":"Br. J. Haematol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"3451","DOI":"10.1245\/s10434-015-4702-1","article-title":"Melanoma: advances in targeted therapy and molecular markers.","volume":"22","author":"DePeralta","year":"2015","journal-title":"Ann. Surg. Oncol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1016\/j.jmoldx.2015.05.007","article-title":"Minimal residual disease detection by droplet digital PCR in multiple myeloma, mantle cell lymphoma, and follicular lymphoma: a comparison with real-time PCR.","volume":"17","author":"Drandi","year":"2015","journal-title":"J. Mol. Diagn."},{"key":"B20","article-title":"Advanced melanoma: a progressive review of V600E-B-RAF kinase and drugs inhibiting it.","author":"El-Gamal","year":"2016","journal-title":"Mini Rev. Med. Chem."},{"key":"B21","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1002\/cncr.23032","article-title":"Long-term follow-up after purine analogue therapy in hairy cell leukaemia.","volume":"28","author":"Else","year":"2015","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1503","DOI":"10.1007\/s00277-013-1797-y","article-title":"Rituximab-based pre-emptive treatment of molecular relapse in follicular and mantle cell lymphoma.","volume":"92","author":"Ferrero","year":"2013","journal-title":"Ann. Hematol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1448","DOI":"10.1038\/sj.bjc.6605277","article-title":"NFAT signalling is a novel target of oncogenic BRAF in metastatic melanoma.","volume":"101","author":"Flockhart","year":"2009","journal-title":"Br. J. Cancer"},{"key":"B24","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1111\/ijlh.12404","article-title":"Real-time PCR and droplet digital PCR: two techniques for detection of the JAK2(V617F) mutation in Philadelphia-negative chronic myeloproliferative neoplasms.","volume":"37","author":"Fontanelli","year":"2015","journal-title":"Int. J. Lab. Hematol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1016\/S0145-2126(99)00110-1","article-title":"An advantageous method to evaluate IgH rearrangement and its role in minimal residual disease detection.","volume":"23","author":"Galimberti","year":"1999","journal-title":"Leuk. Res."},{"key":"B26","doi-asserted-by":"publisher","first-page":"6398","DOI":"10.1158\/1078-0432.CCR-14-0407","article-title":"Minimal residual disease after conventional treatment significantly impacts on progression-free survival of patients with follicular lymphoma: the FIL FOLL05 trial.","volume":"20","author":"Galimberti","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"B27","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.beha.2015.10.015","article-title":"Hairy cell leukemia: past, present and future.","volume":"28","author":"Getta","year":"2015","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"B28","first-page":"455","article-title":"Novel mechanisms and therapeutic approaches in melanoma: targeting the MAPK pathway.","volume":"19","author":"Grimaldi","year":"2015","journal-title":"Discov. Med."},{"key":"B29","doi-asserted-by":"publisher","first-page":"2699","DOI":"10.3892\/ol.2016.4298","article-title":"B-Raf mutation and papillary thyroid carcinoma patients.","volume":"11","author":"Jiang","year":"2016","journal-title":"Oncol. Lett."},{"key":"B30","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1111\/j.1349-7006.1993.tb02864.x","article-title":"B-raf oncogene: activation by rearrangements and assignment to human chromosome 7.","volume":"84","author":"Kamiyama","year":"1993","journal-title":"Jpn. J. Cancer Res."},{"key":"B31","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1007\/s11899-013-0167-0","article-title":"Hairy cell leukemia-new genes, new targets.","volume":"8","author":"Kreitman","year":"2013","journal-title":"Curr. Hematol. Malig. Rep."},{"key":"B32","doi-asserted-by":"publisher","first-page":"e66","DOI":"10.3324\/haematol.2015.132811","article-title":"Positron emission tomography response and minimal residual disease impact on progression-free survival in patients with follicular lymphoma. A subset analysis from the FOLL05 trial of the Fondazione Italiana Linfomi.","volume":"101","author":"Luminari","year":"2016","journal-title":"Haematologica"},{"key":"B33","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1677\/erc.0.0080219","article-title":"Discovery of a novel Raf kinase inhibitor.","volume":"8","author":"Lyons","year":"2001","journal-title":"Endocr. Relat. Cancer"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2016.1185786","article-title":"Detection of activating MAP2K1 mutations in atypical hairy cell leukemia and hairy cell leukemia variant.","author":"Mason","year":"2016","journal-title":"Leuk. Lymphoma"},{"key":"B35","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/S0046-8177(99)90010-2","article-title":"Somatic mutation analysis of IgH variable regions reveals that tumor cells of most parafollicular (monocytoid) B-cell lymphoma, splenic marginal zone B-cell lymphoma, and some hairy cell leukemia are composed of memory B lymphocytes.","volume":"30","author":"Miranda","year":"1999","journal-title":"Hum. Pathol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1002\/ajh.24120","article-title":"Age and dPCR can predict relapse in CML patients who discontinued imatinib: the ISAV study.","volume":"90","author":"Mori","year":"2015","journal-title":"Am. J. Hematol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1177\/2040620715614529","article-title":"Monitoring minimal residual disease in acute myeloid leukaemia: a review of the current evolving strategies.","volume":"7","author":"Ommen","year":"2016","journal-title":"Ther. Adv. Hematol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1097\/PAT.0b013e328360b61d","article-title":"Clinicopathological relevance of BRAF mutations in human cancer.","volume":"45","author":"Pakneshan","year":"2013","journal-title":"Pathology"},{"key":"B39","doi-asserted-by":"publisher","first-page":"3215","DOI":"10.1182\/blood-2009-06-230250","article-title":"Molecular remission is an independent predictor of clinical outcome in patients with mantle cell lymphoma after combined immunochemotherapy: a European MCL intergroup study.","volume":"115","author":"Pott","year":"2010","journal-title":"Blood"},{"key":"B40","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.beha.2015.09.005","article-title":"Chemoimmunotherapy for hairy cell leukemia.","volume":"28","author":"Ravandi","year":"2015","journal-title":"Best Pract. Res. Clin. Haematol."},{"key":"B41","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/0145-2126(95)00165-4","article-title":"The value of the monoclonal antibody, DBA44, in the diagnosis of B-lymphoid disorders.","volume":"20","author":"Salomon-Nguyen","year":"1996","journal-title":"Leuk. Res."},{"key":"B42","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1309\/G629-9513-NGLC-UB1K","article-title":"Minimal residual disease detection in hairy cell leukemia. Comparison of flow cytometric immunophenotyping with clonal analysis using consensus primer polymerase chain reaction for the heavy chain gene.","volume":"119","author":"Sausville","year":"2003","journal-title":"Am. J. Clin. Pathol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"3151","DOI":"10.1182\/blood-2011-10-383323","article-title":"Development and validation of a real-time quantification assay to detect and monitor BRAFV600E mutations in hairy cell leukemia.","volume":"119","author":"Schnittger","year":"2012","journal-title":"Blood"},{"key":"B44","doi-asserted-by":"publisher","first-page":"65","DOI":"10.3109\/10428194.2011.566393","article-title":"Implications of minimal residual disease in hairy cell leukemia after cladribine using immunohistochemistry and immunophenotyping.","volume":"52(Suppl. 2)","author":"Tallman","year":"2011","journal-title":"Leuk. Lymphoma"},{"key":"B45","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1056\/NEJMoa1014209","article-title":"BRAF mutations in hairy-cell leukemia.","volume":"364","author":"Tiacci","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"B46","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1007\/s11523-014-0330-0","article-title":"Targeting BRAF mutant metastatic colorectal cancer: clinical implications and emerging therapeutic strategies.","volume":"10","author":"Tie","year":"2015","journal-title":"Target. Oncol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1038\/sj.leu.2402922","article-title":"Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects.","volume":"17","author":"van der Velden","year":"2003","journal-title":"Leukemia"},{"key":"B48","doi-asserted-by":"publisher","first-page":"9236","DOI":"10.1073\/pnas.96.16.9236","article-title":"Digital PCR.","volume":"96","author":"Vogelstein","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B49","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1007\/s00428-015-1802-z","article-title":"BCR-ABL1 e6a2 transcript in chronic myeloid leukemia: biological features and molecular monitoring by droplet digital PCR.","volume":"467","author":"Zagaria","year":"2015","journal-title":"Virchows Arch."}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fphar.2016.00363\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,25]],"date-time":"2017-06-25T01:00:09Z","timestamp":1498352409000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fphar.2016.00363\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,13]]},"references-count":49,"URL":"https:\/\/doi.org\/10.3389\/fphar.2016.00363","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,13]]}}}