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Pharmacol."],"abstract":"<jats:p>Spider venoms include various peptide toxins that modify the ion currents, mainly of excitable insect cells. Consequently, scientific research on spider venoms has revealed a broad range of peptide toxins with different pharmacological properties, even for mammal species. In this work, thirty animal venoms were screened against hK<jats:sub>v<\/jats:sub>1.5, a potential target for atrial fibrillation therapy. The whole venom of the spider <jats:italic>Oculicosa supermirabilis<\/jats:italic>, which is also insecticidal to house crickets, caused voltage-gated potassium ion channel modulation in hK<jats:sub>v<\/jats:sub>1.5. Therefore, a peptide from the spider <jats:italic>O. supermirabilis<\/jats:italic> venom, named Osu1, was identified through HPLC reverse-phase fractionation. Osu1 displayed similar biological properties as the whole venom; so, the primary sequence of Osu1 was elucidated by both of N-terminal degradation and endoproteolytic cleavage. Based on its primary structure, a gene that codifies for Osu1 was constructed <jats:italic>de novo<\/jats:italic> from protein to DNA by reverse translation. A recombinant Osu1 was expressed using a pQE30 vector inside the <jats:italic>E. coli<\/jats:italic> SHuffle expression system. recombinant Osu1 had voltage-gated potassium ion channel modulation of human hK<jats:sub>v<\/jats:sub>1.5, and it was also as insecticidal as the native toxin. Due to its novel primary structure, and hypothesized disulfide pairing motif, Osu1 may represent a new family of spider toxins.<\/jats:p>","DOI":"10.3389\/fphar.2020.563858","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T08:28:38Z","timestamp":1610612918000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["A Novel Insecticidal Spider Peptide that Affects the Mammalian Voltage-Gated Ion Channel hKv1.5"],"prefix":"10.3389","volume":"11","author":[{"given":"Diana","family":"Alvarado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Cardoso-Arenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligia-Luz","family":"Corrales-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herlinda","family":"Clement","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Arenas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel Andrei","family":"Montero-Dominguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timoteo","family":"Olamendi-Portugal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Zamudio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agota","family":"Csoti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Borrego","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Panyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferenc","family":"Papp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"Corzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/j.csbj.2019.01.012","article-title":"Stapled peptides inhibitors: a new window for target drug discovery","volume":"17","author":"Ali","year":"2019","journal-title":"Comput. 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