{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T06:14:03Z","timestamp":1764828843067,"version":"3.41.2"},"reference-count":72,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T00:00:00Z","timestamp":1611878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Scientists have been constantly looking for new synthetic and natural compounds that could have beneficial effects in bladder overactivity. Our attention was drawn by asiatic acid that influences a number of molecules and signaling pathways relevant for the proper functioning of the urinary tracts in humans. In the present project we wanted to check whether asiatic acid would have positive effects in the confirmed animal model of detrusor overactivity (DO) and whether it would affect the bladder blood flow, urothelium thickness, inflammatory and oxidative stress markers, neurotrophic and growth factors, and other parameters important for the activity of the urinary bladder. The outcomes of our study showed that a 14-day administration of asiatic acid (30\u00a0mg\/kg\/day) by oral gavage normalizes the cystometric parameters corresponding to DO and reduces the accompanying oxidative stress (measured by the levels of malondialdehyde\u201361,344 \u00b1 24,908\u00a0pg\/ml vs. 33,668 \u00b1 5,071\u00a0pg\/ml, 3-nitrotyrosine\u201364,615 \u00b1 25,433\u00a0pg\/ml vs. 6,563 \u00b1 1,736\u00a0pg\/ml, and NOS2\u20132,506 \u00b1 411.7 vs. 3,824 \u00b1 470.1\u00a0pg\/ml). Moreover, it decreases the urinary secretion of neurotrophins (BDNF\u2013304.4 \u00b1 33.21\u00a0pg\/ml vs. 119.3 \u00b1 11.49\u00a0pg\/ml and NGF\u2013205.5 \u00b1 18.50 vs. 109.7 \u00b1 15.94\u00a0pg\/ml) and prevents the changes in a range of biomarkers indicating the dysfunction of the urinary bladder, CGRP (421.1 \u00b1 56.64 vs. 108.1 \u00b1 11.73\u00a0pg\/ml), E-Cadherin (773.5 \u00b1 177.5\u00a0pg\/ml vs. 1,560 \u00b1 154.5\u00a0pg\/ml), OCT3 (3,943 \u00b1 814.6 vs. 1,018 \u00b1 97.07\u00a0pg\/ml), SNAP-23 (6,763 \u00b1 808.9\u00a0pg\/ml vs. 3,455 \u00b1 554.5\u00a0pg\/ml), SNAP-25 (2,038 \u00b1 162.7\u00a0pg\/ml vs. 833.3 \u00b1 65.48), substance P (171.7 \u00b1 16.86\u00a0pg\/ml vs. 65.07 \u00b1 8.250\u00a0pg\/ml), SV2A (1,927 \u00b1 175.3\u00a0pg\/ml vs. 1,154 \u00b1 254.9\u00a0pg\/ml), tight junction protein 1 (360.1 \u00b1 95.05\u00a0pg\/ml vs. 563.4 \u00b1 65.43\u00a0pg\/ml), VAChT (16,470 \u00b1 2,419\u00a0pg\/ml vs. 7,072 \u00b1 1,339\u00a0pg\/ml), VEGFA (318.3 \u00b1 37.89\u00a0pg\/ml vs. 201.5 \u00b1 22.91\u00a0pg\/ml). The mentioned parameters are associated with smooth muscle contractions, urothelial barrier, transportation and release of transmitters, or bladder compensation. Thus, the presented findings allow to suggest a possible future role of asiatic acid in the prevention of conditions accompanied by DO, such as overactive bladder.<\/jats:p>","DOI":"10.3389\/fphar.2020.574108","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T05:08:11Z","timestamp":1611896891000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Asiatic Acid, a Natural Compound that Exerts Beneficial Effects on the Cystometric and Biochemical Parameters in the Retinyl Acetate-Induced Model of Detrusor Overactivity"],"prefix":"10.3389","volume":"11","author":[{"given":"Andrzej","family":"Wr\u00f3bel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Serefko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Szopa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Poleszak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,1,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1067\/mob.2002.125704","article-title":"The standardisation of terminology of lower urinary tract function: report from the Standardisation Sub-committee of the International Continence Society","volume":"187","author":"Abrams","year":"2002","journal-title":"Am. 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