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Pharmacol."],"abstract":"<jats:p>Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20\u201330\u00a0kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged <jats:italic>Philodryas patagoniensis<\/jats:italic>. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS\/MS, and a protein of 24,848.8\u00a0Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of <jats:italic>P<\/jats:italic>. <jats:italic>patagoniensis<\/jats:italic> had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for <jats:italic>P<\/jats:italic>. <jats:italic>patagoniensis<\/jats:italic> venom or for a CRISP family protein.<\/jats:p>","DOI":"10.3389\/fphar.2020.586705","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T21:06:13Z","timestamp":1612299973000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy\u2019s Gland Secretion (Philodryas patagoniensis Snake)"],"prefix":"10.3389","volume":"11","author":[{"given":"Juliana Cuoco","family":"Badari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"D\u00edaz-Roa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa Maria","family":"Teixeira Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo Zucatelli","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Ismael da","family":"Silva Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e3252","DOI":"10.1371\/journal.pntd.0003252","article-title":"Crovirin, a snake venom cysteine-rich secretory protein (CRISP) with promising activity against Trypanosomes and Leishmania","volume":"8","author":"Adade","year":"2014","journal-title":"PLoS Negl. 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