{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:51:06Z","timestamp":1787503866817,"version":"build-2736575974"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:00:00Z","timestamp":1613606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>\n                    <jats:italic>Bryophyllum pinnatum<\/jats:italic>\n                    has been used in the treatment of premature labor, first in anthroposophic hospitals and, recently, in conventional settings as an add-on medication.\n                    <jats:italic>In vitro<\/jats:italic>\n                    work with hTERT human myometrial cells showed that\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    leaf press juice inhibits the increase of intracellular free calcium concentration induced by oxytocin, a hormone known to play a role in labor. Our aim was to identify fractions\/compounds in\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice that contribute to this inhibitory effect, and to investigate their effect on oxytocin-driven activation of the MAPK cascade. Several fractions\/compounds from\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice led to a concentration-dependent decrease of oxytocin-induced increase of intracellular free calcium concentration, but none of them was as strong as\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice. However, the combination of a bufadienolide and a flavonoid-enriched fraction was as effective as\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice, and their combination had a synergistic effect.\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice inhibited oxytocin-driven activation of MAPKs SAPK\/JNK and ERK1\/2, an effect also exerted by the bufadienolide-enriched fraction. The effect of\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice on oxytocin-induced signaling pathways was comparable to that of the oxytocin-receptor antagonist and tocolytic agent atosiban. Our findings further substantiate the use of\n                    <jats:italic>B. pinnatum<\/jats:italic>\n                    press juice preparations in the treatment of preterm labor.\n                  <\/jats:p>","DOI":"10.3389\/fphar.2021.632986","type":"journal-article","created":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T12:30:17Z","timestamp":1613824217000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Bryophyllum pinnatum Compounds Inhibit Oxytocin-Induced Signaling Pathways in Human Myometrial Cells"],"prefix":"10.3389","volume":"12","author":[{"given":"Stefanie","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonie","family":"Zurfluh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Mennet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Potterat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"von Mandach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Hamburger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Sim\u00f5es-W\u00fcst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,2,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.ogrm.2010.05.001","article-title":"Drugs acting on the pregnant uterus","volume":"20","author":"Arrowsmith","year":"2010","journal-title":"Obstet. 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