{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:14:07Z","timestamp":1792516447739,"version":"4.5.0"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T00:00:00Z","timestamp":1626220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008436","name":"National Foundation for Medical Research and Innovation","doi-asserted-by":"publisher","award":["No. 2016YFC1302200 No. 81873544"],"award-info":[{"award-number":["No. 2016YFC1302200 No. 81873544"]}],"id":[{"id":"10.13039\/100008436","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>\n                    As little is known about the role of calcium (Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ) signaling mediating the small intestinal epithelial anion secretion, we aimed to study its regulatory role in secretagogue-stimulated duodenal anion secretion and the underlying molecular mechanisms. Therefore, intestinal anion secretion from native mouse duodenal epithelia was examined with Ussing chambers to monitor PGE\n                    <jats:sub>2<\/jats:sub>\n                    -, 5-HT-, and CCh-induced short-circuit currents (\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    ). PGE\n                    <jats:sub>2<\/jats:sub>\n                    (10\u00a0\u03bcM) and 5-HT (10\u00a0\u03bcM) induced mouse duodenal\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    , markedly attenuated by serosal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free solution and selective blockers of store-operated Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels on the serosal side of the duodenum. Furthermore, PGE\n                    <jats:sub>2<\/jats:sub>\n                    - and 5-HT-induced duodenal\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    was also inhibited by ER Ca\n                    <jats:sup>2+<\/jats:sup>\n                    chelator TPEN. However, dantrolene, a selective blocker of ryanodine receptors, inhibited PGE\n                    <jats:sub>2<\/jats:sub>\n                    -induced duodenal\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    , while LiCl, an inhibitor of IP\n                    <jats:sub>3<\/jats:sub>\n                    production, inhibited 5-HT-induced\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    . Moreover, duodenal\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    response to the serosal applications of both PGE\n                    <jats:sub>2<\/jats:sub>\n                    and 5-HT was significantly attenuated in transient receptor potential vanilloid 4 (TRPV4) knockout mice. Finally, mucosal application of carbachol (100\u00a0\u03bcM) also induced duodenal\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>sc<\/jats:italic>\n                    <\/jats:sub>\n                    via selective activation of muscarinic receptors, which was significantly inhibited in serosal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free solution but neither in mucosal Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -free solution nor by nifedipine. Therefore, the serosal TRPV4-constituted SOCE mechanism is likely universal for the most common and important secretagogues-induced and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent intestinal anion secretion. These findings will enhance our knowledge about gastrointestinal (G.I.) epithelial physiology and the associated G.I. diseases, such as diarrhea and constipation.\n                  <\/jats:p>","DOI":"10.3389\/fphar.2021.684538","type":"journal-article","created":{"date-parts":[[2021,7,14]],"date-time":"2021-07-14T08:18:46Z","timestamp":1626250726000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Role of Serosal TRPV4-Constituted SOCE Mechanism in Secretagogues-Stimulated Intestinal Epithelial Anion Secretion"],"prefix":"10.3389","volume":"12","author":[{"given":"Yinghui","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenglan","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxing","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,7,14]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"20860","DOI":"10.1074\/jbc.M302599200","article-title":"Long Distance Communication between Muscarinic Receptors and Ca2+ Release Channels Revealed by Carbachol Uncaging in Cell-Attached Patch Pipette","volume":"278","author":"Ashby","year":"2003","journal-title":"J. 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