{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T10:07:00Z","timestamp":1775297220430,"version":"3.50.1"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>The aim of this study is determine the <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> antiradical properties and the cytoprotective activity of <jats:italic>Allium nutans<\/jats:italic> L. honey extract. The antiradical properties of the extracts were investigated in rabbit alveolar macrophages and human foreskin fibroblast (hFFs) cells in the presence of doxorubicin, a cytotoxic substance using DPPH and ABTS assays. The cytoprotective activities were determined using 18 Wistar rats divided into three different groups, a negative control, and two other groups with experimentally induced hepatotoxicity by a single intraperitoneal injection of 50% carbon tetrachloride (CCl4) oil solution. A positive control group, received drinking water only and an experimental group that was treated with <jats:italic>Allium nutans<\/jats:italic> L. honey extracts for 7\u00a0days. <jats:italic>In vitro<\/jats:italic> treatment with <jats:italic>Allium nutans<\/jats:italic> L. honey extracts resulted in 78% reduction in radical activity in DPPH and 91.6% inhibition using the ABTS. Also, honey extracts were able to preserve 100% of cell viability in the presence of the cytotoxic, doxorubicin. Furthermore, the treatment with honey extracts resulted in a significant reduction in damage to the structure of liver tissue, as well significant reduction in the levels of ALT and AST in the experimental group compared to the control group.<\/jats:p>","DOI":"10.3389\/fphar.2021.687763","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T05:13:00Z","timestamp":1632114780000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Antiradical and Cytoprotective Properties of Allium nutans L. Honey Against CCL4-Induced Liver Damage in Rats"],"prefix":"10.3389","volume":"12","author":[{"given":"Shynggys","family":"Sergazy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Gulyayev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aidana","family":"Amangeldiyeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayaulym","family":"Nurgozhina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madiyar","family":"Nurgaziyev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zarina","family":"Shulgau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Chulenbayeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanagul","family":"Khassenbekova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almagul","family":"Kushugulova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad","family":"Aljofan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.freeradbiomed.2018.07.014","article-title":"Manuka Honey Synergistically Enhances the Chemopreventive Effect of 5-Fluorouracil on Human Colon Cancer Cells by Inducing Oxidative Stress and Apoptosis, Altering Metabolic Phenotypes and Suppressing Metastasis Ability","volume":"126","author":"Afrin","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1204","DOI":"10.18016\/ksutarimdoga.vi.626803","article-title":"Determining the Total Antioxidant Status and Oxidative Stress Indexes of Honey Samples Obtained from Different Phytogeographical Regions in Turkey","volume":"4","author":"Akyol","year":"2015","journal-title":"Fresenius Environ. Bull."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2013\/569037","article-title":"Attenuation of CClinduced Oxidative Stress and Hepatonephrotoxicity by Saudi Sidr Honey in Rats. Evidence-Based Complement","volume":"2013","author":"Al-Yahya","year":"2013","journal-title":"Altern. Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.4314\/bajopas.v2i2.63809","article-title":"Ideal Hepatotoxicity Model in Rats Using Carbon","volume":"2","author":"Alhassan","year":"2009","journal-title":"Bayero J. Pure Appl. Sci."},{"key":"B5","doi-asserted-by":"publisher","first-page":"621","DOI":"10.2174\/092986713804999358","article-title":"Honey as a Source of Dietary Antioxidants: Structures, Bioavailability and Evidence of Protective Effects against Human Chronic Diseases","volume":"20","author":"Alvarez-Suarez","year":"2013","journal-title":"Curr. Med. Chem."},{"key":"B6","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.jff.2016.05.008","article-title":"Activation of AMPK\/Nrf2 Signalling by Manuka Honey Protects Human Dermal Fibroblasts against Oxidative Damage by Improving Antioxidant Response and Mitochondrial Function Promoting Wound Healing","volume":"25","author":"Alvarez-Suarez","year":"2016","journal-title":"J. Funct. Foods"},{"key":"B7","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.chroma.2014.01.081","article-title":"Determination of Phenolic Compounds in Honey Using Dispersive Liquid-Liquid Microextraction","volume":"1334","author":"Campone","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"B8","doi-asserted-by":"publisher","first-page":"273","DOI":"10.24925\/turjaf.v2i6.273-276.151","article-title":"Biologic Activities of Honeybee Products Obtained from Different Phytogeographical Regions of Turkey","volume":"2","author":"Do\u011fan","year":"2014","journal-title":"Turkish J. Agric. - Food Sci. Technol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/j.etp.2011.01.012","article-title":"Dietary Honey and Ginseng Protect against Carbon Tetrachloride-Induced Hepatonephrotoxicity in Rats","volume":"64","author":"El Denshary","year":"2012","journal-title":"Exp. Toxicol. Pathol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.clinbiochem.2003.11.015","article-title":"A Novel Automated Direct Measurement Method for Total Antioxidant Capacity Using a New Generation, More Stable ABTS Radical Cation","volume":"37","author":"Erel","year":"2004","journal-title":"Clin. Biochem."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1007\/s13197-019-04159-w","article-title":"Antioxidant Properties and Antimicrobial Activity of Manuka Honey versus Polish Honeys","volume":"57","author":"Go\u015bli\u0144ski","year":"2020","journal-title":"J. Food Sci. Technol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"315","DOI":"10.4314\/ajtcam.v7i4.56693","article-title":"The Potential Role of Honey and its Polyphenols in Preventing Heart Disease: A Review","volume":"7","author":"Khalil","year":"2010","journal-title":"Afr. J. Trad. Compl. Alt. Med."},{"key":"B13","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1515\/hsz-2013-0241","article-title":"The Role of Oxidative Stress during Inflammatory Processes","volume":"395","author":"Lugrin","year":"2014","journal-title":"Biol. Chem."},{"key":"B14","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/S2221-1691(11)60016-6","article-title":"Honey: Its Medicinal Property and Antibacterial Activity","volume":"1","author":"Mandal","year":"2011","journal-title":"Asian Pac. J. Trop. Biomed."},{"key":"B15","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.taap.2011.10.016","article-title":"Insights Antifibrotic Mechanism of Methyl Palmitate: Impact on Nuclear Factor Kappa B and Proinflammatory Cytokines","volume":"258","author":"Mantawy","year":"2012","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"167","DOI":"10.3390\/nu11010167","article-title":"Honey and its Role in Relieving Multiple Facets of Atherosclerosis","volume":"11","author":"Nguyen","year":"2019","journal-title":"Nutrients"},{"key":"B17","doi-asserted-by":"publisher","first-page":"283","DOI":"10.3390\/nu12020283","article-title":"Honey and its Phenolic Compounds as an Effective Natural Medicine for Cardiovascular Diseases in Humans?","volume":"12","author":"Olas","year":"2020","journal-title":"Nutrients"},{"key":"B18","doi-asserted-by":"publisher","first-page":"306","DOI":"10.3390\/foods9030306","article-title":"Antioxidant Activity, Total Phenolic Content, Individual Phenolics and Physicochemical Parameters Suitability for Romanian Honey Authentication","volume":"9","author":"Pauliuc","year":"2020","journal-title":"Foods"},{"key":"B19","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1016\/j.trac.2009.03.015","article-title":"Analysis of Phenolic Acids and Flavonoids in Honey","volume":"28","author":"Pyrzynska","year":"2009","journal-title":"Trac Trends Anal. Chem."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"B21","doi-asserted-by":"publisher","first-page":"927","DOI":"10.2174\/1389450118666170328122527","article-title":"Natural Polyphenols in the Treatment of Alzheimer's Disease","volume":"19","author":"Syarifah-Noratiqah","year":"2018","journal-title":"Curr. Drug Targets"},{"key":"B22","doi-asserted-by":"publisher","first-page":"356","DOI":"10.3390\/insects10100356","article-title":"Natural Product Medicines for Honey Bees: Perspective and Protocols","volume":"10","author":"Tauber","year":"2019","journal-title":"Insects"},{"key":"B23","doi-asserted-by":"publisher","first-page":"C343","DOI":"10.1111\/j.1750-3841.2007.00415.x","article-title":"Phenolic Acid Content and Composition in Leaves and Roots of Common Commercial Sweetpotato (Ipomea Batatas L.) Cultivars in the United States","volume":"72","author":"Truong","year":"2007","journal-title":"J. Food Sci."},{"key":"B24","doi-asserted-by":"publisher","first-page":"2499","DOI":"10.1016\/j.clnu.2018.12.019","article-title":"Honey and Cancer: A Mechanistic Review","volume":"38","author":"Waheed","year":"2019","journal-title":"Clin. Nutr."},{"key":"B25","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1080\/713611034","article-title":"Hepatotoxicity and Mechanism of Action of Haloalkanes: Carbon Tetrachloride as a Toxicological Model","volume":"33","author":"Weber","year":"2003","journal-title":"Crit. Rev. Toxicol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"137","DOI":"10.4172\/2573-4555.1000e138","article-title":"Polyphenolic Compounds in Human Health with Pharmacological Properties","volume":"6","author":"Zeliha","year":"2017","journal-title":"J. Traditional Med. Clin. Naturopathy"}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2021.687763\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T05:13:01Z","timestamp":1632114781000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2021.687763\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,20]]},"references-count":26,"alternative-id":["10.3389\/fphar.2021.687763"],"URL":"https:\/\/doi.org\/10.3389\/fphar.2021.687763","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,20]]},"article-number":"687763"}}