{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:39:42Z","timestamp":1783618782451,"version":"3.55.0"},"reference-count":33,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T00:00:00Z","timestamp":1630972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004413","name":"International Foundation for Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004413","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Whereas the efficacy of <jats:italic>Entada abyssinica<\/jats:italic> (fabaceae) extracts against various ailments has been scientifically validated, its safety has not been established. This study was undertaken to evaluate the toxicity effects of methanolic stem bark extract of <jats:italic>E. abyssinica<\/jats:italic> on biochemical, haematological and histological parameters of Wistar albino rats following repeated oral administration.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Wistar albino rats of both sexes were randomized into groups and orally administered daily with determined doses (150, 300 and 600\u00a0mg\/kg) of <jats:italic>E. abyssinica<\/jats:italic> methanolic extract using 1% tween 80 in distilled water as a control for 28\u00a0days. On the 29th day, all the animals were sacrificed and dissected to collect blood and selected organs. The serum and whole blood were assayed for biochemical and haematological parameters respectively while selected organs were examined for histopathological lesions. Numerical data was analyzed using graph pad prism and expressed as mean \u00b1 standard error of mean. The differences between the treatment and control groups were tested for statistical significance using one-way analysis of variance and\/or Student\u2019s <jats:italic>t-<\/jats:italic>test.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In repeated daily oral doses (150, 300 and 600\u00a0mg\/kg), the methanolic stem bark extract of <jats:italic>E. abyssinica<\/jats:italic> did not cause significant alteration in majority of the biochemical and hematological indices. However, the extract significantly elevated the level of uric acid (all doses), aspartate aminotransferase (300 and 600\u00a0mg\/kg), low density lipoproteins (150\u00a0mg\/kg) and mean corpuscular heamoglobin concentration (all doses). On the other hand, the extracts reduced high density lipoproteins (150 and 300\u00a0mg\/kg), mean corpuscular volume (all doses), haematocrit (150 and 600\u00a0mg\/kg), mean platelet volume (150 and 600\u00a0mg\/kg) and procalcitonin (150\u00a0mg\/kg). In the vital organs, there were no significant lesions observed except at the highest dose (600\u00a0mg\/kg) where there was mild evidence of lymphocyte infiltration in the liver and focal interstitial nephritis.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The methanolic stem bark extract of <jats:italic>E. abyssinica<\/jats:italic> is relatively safe in Wistar albino rats when repetitively administered orally in small doses for a prolonged period of time. We recommend more chronic toxicity studies and clinical trials on herbal remedies containing this plant to ensure that its use is free of potential toxicity to humans.<\/jats:p>","DOI":"10.3389\/fphar.2021.740305","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T05:03:24Z","timestamp":1630991004000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":27,"title":["Sub-Acute Toxicity Effects of Methanolic Stem Bark Extract of Entada abyssinica on Biochemical, Haematological and Histopathological Parameters in Wistar Albino Rats"],"prefix":"10.3389","volume":"12","author":[{"given":"Samuel Baker","family":"Obakiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ambrose","family":"Kiprop","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Kigondu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"K\u2019owino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenedy","family":"Kiyimba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Drago Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yahaya","family":"Gavamukulya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/antibiotics9090601","article-title":"Haematology and Serum Biochemical Indices of Lambs Supplemented with Moringa Oleifera, Jatropha Curcas and Aloe Vera Leaf Extract as Anti-methanogenic Additives","volume":"9","author":"Akanmu","year":"2020","journal-title":"Antibiotics (Basel)."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphar.2021.615147","article-title":"A Review of the Toxicity and Phytochemistry of Medicinal Plant Species Used by Herbalists in Treating People Living with HIV\/AIDS in Uganda","volume":"12","author":"Anywar","year":"2021","journal-title":"Front. 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