{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:01:48Z","timestamp":1779328908136,"version":"3.51.4"},"reference-count":74,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T00:00:00Z","timestamp":1636070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Age-related macular degeneration (AMD) is a degenerative retinal disease and one of major causes of irreversible vision loss. AMD has been linked to several pathological factors, such as oxidative stress and inflammation. Moreover, A\u03b2 (1\u201342) oligomers have been found in drusen, the extracellular deposits that accumulate beneath the retinal pigmented epithelium in AMD patients. Hereby, we investigated the hypothesis that treatment with 1,25(OH) <jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> (vitamin D<jats:sub>3<\/jats:sub>) and meso-zeaxathin, physiologically present in the eye, would counteract the toxic effects of three different insults on immortalized human retinal pigmented epithelial cells (ARPE-19). Specifically, ARPE-19 cells have been challenged with A\u03b2 (1\u201342) oligomers, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, LPS, and TNF-\u03b1, respectively. In the present study, we demonstrated that the combination of 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and meso-zeaxanthin significantly counteracted the cell damage induced by the three insults, at least in these <jats:italic>in vitro<\/jats:italic> integrated paradigms of AMD. These results suggest that combination of 1,25(OH)<jats:sub>2<\/jats:sub>D<jats:sub>3<\/jats:sub> and meso-zeaxathin could be a useful approach to contrast pathological features of AMD, such as retinal inflammation and oxidative stress.<\/jats:p>","DOI":"10.3389\/fphar.2021.778165","type":"journal-article","created":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T08:30:00Z","timestamp":1636101000000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration"],"prefix":"10.3389","volume":"12","author":[{"given":"Francesca","family":"Lazzara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Conti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara Bianca Maria","family":"Platania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara M.","family":"Eandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Drago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Bucolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,11,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(01)01218-1","article-title":"The Age-Related Eye Disease Study System for Classifying Age-Related Macular Degeneration from Stereoscopic Color Fundus Photographs: The Age-Related Eye Disease Study Report Number","year":"2001","journal-title":"Am. 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