{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T04:02:57Z","timestamp":1771905777051,"version":"3.50.1"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T00:00:00Z","timestamp":1648684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005199","name":"Chinese Medicine Research Program of Zhejiang Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Allergic asthma is a common respiratory inflammation disease. The crude Radix Paeoniae Alba (RPA) and its processed products have been used frequently as antipyretic and anti-inflammatory agents in traditional medicine. To evaluate the effect of honey and bran processing, different fractions of RPA were used for treating anti-allergic asthma in the ovalbumin (OVA)-induced mice model, and then, the most effective fraction of RPA and stir-frying Radix Paeoniae Alba with honey and bran (FRPA) for treating anti-allergic asthma were compared mutually for pharmacological effects. The results showed that the treatment of the dichloromethane fraction of RPA significantly improved the pathological condition of lung tissues, decreased the number of eosinophils and other cells in bronchoalveolar lavage fluid (BALF), and the increased the expression of various inflammatory factors. Furthermore, the study discovered that the lung pathological conditions, compared with the high dose of dichloromethane RPA fraction, could be ameliorated by high dose of dichloromethane FRPA fraction treatment. Moreover, the expression of inflammatory factors and the phosphorylation of the PI3K\/AKT signaling pathway could be diminished by FRPA. Finally, the contents of compounds with a significant difference in the FRPA dichloromethane fraction were paeoniflorin, ethyl gallate, pentagalloylglucose, galloylpaeoniflorin, and others by UPLC\/Q-TOF-MS analysis. These findings suggest that the dichloromethane fraction of FRPA has an enhancement effect on anti-allergic asthma and provide the experimental basis for exploring the processed mechanism of RPA.<\/jats:p>","DOI":"10.3389\/fphar.2022.863403","type":"journal-article","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T16:12:54Z","timestamp":1648743174000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Screening of Bioactive Fraction of Radix Paeoniae Alba and Enhancing Anti-Allergic Asthma by Stir-Frying Through Regulating PI3K\/AKT Signaling Pathway"],"prefix":"10.3389","volume":"13","author":[{"given":"Xia\u2019nan","family":"Sang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jialiang","family":"Ying","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuedong","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiyuan","family":"Shan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Lyu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuilong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Hao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erlong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.18502\/rmm.v6i1.3926","article-title":"Immune Dysregulation in Children with Allergic Asthma: A Close Relationship between IL-17 but Not IL-4 or IFN-","volume":"6","author":"Aghajani","year":"2019","journal-title":"RMM"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e12998","DOI":"10.1111\/sji.12998","article-title":"Coronavirus Disease 2019 (COVID\u201019): An Overview of the Immunopathology, Serological Diagnosis and Management","volume":"93","author":"Anka","year":"2021","journal-title":"Scand. J. Immunol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"3134","DOI":"10.1039\/c9fo02406a","article-title":"Chemical Basis of Hawthorn Processed with Honey Protecting against Myocardial Ischaemia","volume":"11","author":"Ao","year":"2020","journal-title":"Food Funct."},{"key":"B4","doi-asserted-by":"publisher","first-page":"3548","DOI":"10.3969\/j.issn.1005-9202.2020.16.056","article-title":"Galloylpaeoniflorin Reduces PM2.5-induced Injury of Vascular Endothelial Cells by Activating the Nrf2 Signaling Pathway[J]","volume":"40","author":"Bi","year":"2020","journal-title":"Chin. J. Gerontol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1038\/s41374-021-00618-1","article-title":"Surfactant Protein A Modulates the Activities of the JAK\/STAT Pathway in Suppressing Th1 and Th17 Polarization in Murine OVA-Induced Allergic Asthma","volume":"101","author":"Chen","year":"2021","journal-title":"Lab. Invest."},{"key":"B6","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/s10238-020-00671-y","article-title":"Emerging Treatment Strategies for COVID-19 Infection","volume":"21","author":"Gavriatopoulou","year":"2021","journal-title":"Clin. Exp. Med."},{"key":"B7","doi-asserted-by":"publisher","first-page":"50","DOI":"10.16588\/j.cnki.issn2096-8426.2021.02.010","article-title":"Study on Quality Standard of Radix Paeoniae Alba and its Processed Pieces[J]","volume":"43","author":"Gu","year":"2021","journal-title":"J. Guizhou.Univ. Tradit. Chin. Med."},{"key":"B8","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1067\/mai.2003.22","article-title":"Inflammatory Cells in Asthma: Mechanisms and Implications for Therapy","volume":"111","author":"Hamid","year":"2003","journal-title":"J. Allergy Clin. Immunol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"63","DOI":"10.3969\/j.issn.1006-1959.2021.18.017","article-title":"Study on Drug Treatment of Asthma[J]","volume":"34","author":"Han","year":"2021","journal-title":"Med. Inf."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1210","DOI":"10.1111\/irv.12150","article-title":"Inactivated Influenza Virus Vaccine Is Efficient and Reduces IL-4 and IL-6 in Allergic Asthma Mice","volume":"7","author":"Jian","year":"2013","journal-title":"Influenza Other Respir. Viruses."},{"key":"B11","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1080\/15287394.2018.1460785","article-title":"Protective Effect of Paeoniae Radix alba Root Extract on Immune Alterations in Mice with Atopic Dermatitis","volume":"81","author":"Jo","year":"2018","journal-title":"J. Toxicol. Environ. Health A."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1895","DOI":"10.1007\/s10753-021-01466-3","article-title":"PI3K\/AKT\/mTOR and TLR4\/MyD88\/NF-\u03baB Signaling Inhibitors Attenuate Pathological Mechanisms of Allergic Asthma","volume":"44","author":"Ma","year":"2021","journal-title":"Inflammation"},{"key":"B13","doi-asserted-by":"publisher","first-page":"S2","DOI":"10.1002\/alr.21609","article-title":"Asthma: Definitions and Pathophysiology","volume":"5","author":"Mims","year":"2015","journal-title":"Int. Forum Allergy Rhinol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/s10439-018-02145-5","article-title":"Pentagalloyl Glucose and its Functional Role in Vascular Health: Biomechanics and Drug-Delivery Characteristics","volume":"47","author":"Patnaik","year":"2019","journal-title":"Ann. Biomed. Eng."},{"key":"B15","doi-asserted-by":"publisher","first-page":"11614","DOI":"10.18632\/oncotarget.14595","article-title":"Asthma and the Risk of Lung Cancer: a Meta-Analysis","volume":"8","author":"Qu","year":"2017","journal-title":"Oncotarget"},{"key":"B16","doi-asserted-by":"publisher","first-page":"268","DOI":"10.3969\/j.issn.1673-7202.2019.02.003","article-title":"Study on Chemical Components of Radix Paeoniae Alba before and after Stir-Frying Based on UHPLC-Q-TOF-MS\/MS[J]","volume":"14","author":"Ren","year":"2019","journal-title":"World Chin. Med."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1002\/ppul.23893","article-title":"Interleukin 1\u03b2 Polymorphism and Serum Level Are Associated with Pediatric Asthma","volume":"52","author":"Sobkowiak","year":"2017","journal-title":"Pediatr. Pulmonol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"94","DOI":"10.13863\/j.issn1001-4454.2017.01.023","article-title":"Study on Rapid Identification of Water-Soluble Component in Radix Paeoniae Alba[J]","volume":"40","author":"Tan","year":"2017","journal-title":"J. Chin. Med. Mater."},{"key":"B19","doi-asserted-by":"publisher","first-page":"113894","DOI":"10.1016\/j.jep.2021.113894","article-title":"Vitex Negundo Linn. Extract Alleviates Inflammatory Aggravation and Lung Injury by Modulating AMPK\/PI3K\/Akt\/p38-NF-\u03baB and TGF-\u03b2\/Smad\/Bcl2\/caspase\/LC3 cascade and Macrophages Activation in Murine Model of OVA-LPS Induced Allergic Asthma","volume":"271","author":"Tirpude","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1038\/s41579-020-00461-z","article-title":"Considerations for Diagnostic COVID-19 Tests","volume":"19","author":"Vandenberg","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"14634","DOI":"10.3390\/ijms131114634","article-title":"Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-Induced Acute Liver Injury: an Ultra-performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) Based Metabolomic Approach for the Pharmacodynamic Study of Traditional Chinese Medicines (TCMs)","volume":"13","author":"Wang","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2021\/9659304","article-title":"Network Pharmacology Analysis of the Identification of Phytochemicals and Therapeutic Mechanisms of Paeoniae Radix Alba for the Treatment of Asthma","volume":"2021","author":"Wang","year":"2021","journal-title":"J. Immunol. Res."},{"key":"B23","doi-asserted-by":"publisher","first-page":"107073","DOI":"10.1016\/j.intimp.2020.107073","article-title":"Alpinetin Prevents Inflammatory Responses in OVA-Induced Allergic Asthma through Modulating PI3K\/AKT\/NF-\u03baB and HO-1 Signaling Pathways in Mice","volume":"89","author":"Wu","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"175","DOI":"10.13220\/j.cnki.jipr.2020.03.003","article-title":"Research Progress on Chemical Components and Pharmacological Effects of Radix Paeoniae Alba[J]","volume":"47","author":"Wu","year":"2020","journal-title":"J. Int. Pharm. Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1002\/PCA.3029","article-title":"Cleavage Rules of Mass Spectrometry Fragments and Rapid Identification of Chemical Components of Radix Paeoniae Alba Using UHPLC\u2010Q\u2010TOF\u2010MS","volume":"32","author":"Yang","year":"2021","journal-title":"Phytochem. Anal."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1951","DOI":"10.7501\/j.issn.0253-2670.2020.07.032","article-title":"Research Progress on Historical Evolution, Chemical Constituents, and Pharmacological Effects of Processed Radix Paeoniae Alba[J]","volume":"51","author":"Ye","year":"2020","journal-title":"Chin. Herb. Med."},{"key":"B27","doi-asserted-by":"publisher","first-page":"102494","DOI":"10.1016\/j.pdpdt.2021.102494","article-title":"Choroidal Vascularity index and Choroidal Thickness Changes in Patients with Allergic Asthma","volume":"36","author":"Y\u0131lmaz","year":"2021","journal-title":"Photodiagnosis Photodynamic Ther."},{"key":"B28","doi-asserted-by":"publisher","first-page":"71","DOI":"10.2147\/DDDT.S186029","article-title":"Protective Effects of Ethyl Gallate and Pentagalloylglucose, the Active Components of Qingwen Baidu Decoction, against Lipopolysaccharide-Induced Acute Lung Injury in Rats","volume":"13","author":"Zhang","year":"2018","journal-title":"Drug Des. Devel Ther."},{"key":"B29","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.intimp.2019.04.033","article-title":"Paeonol: Pharmacological Effects and Mechanisms of Action","volume":"72","author":"Zhang","year":"2019","journal-title":"Int. Immunopharmacol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"113435","DOI":"10.1016\/j.jpba.2020.113435","article-title":"An Integrative Metabolomics and Network Pharmacology Method for Exploring the Effect and Mechanism of Radix Bupleuri and Radix Paeoniae Alba on Anti-depression","volume":"189","author":"Zhang","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"B31","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1080\/14786419.2014.985677","article-title":"Ultra-performance Liquid Chromatography Coupled with High-Resolution Quadrupole Time-Of-Flight Mass Spectrometry Analysis of the Impact of Bran-Processing on the Chemical Profile of Radix Paeoniae Alba (Baishao)","volume":"29","author":"Zheng","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"110505","DOI":"10.1016\/j.biopha.2020.110505","article-title":"A Review on the Pharmacokinetics of Paeoniflorin and its Anti-inflammatory and Immunomodulatory Effects","volume":"130","author":"Zhou","year":"2020","journal-title":"Biomed. Pharmacother."}],"container-title":["Frontiers in Pharmacology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2022.863403\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T16:13:05Z","timestamp":1648743185000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphar.2022.863403\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,31]]},"references-count":32,"alternative-id":["10.3389\/fphar.2022.863403"],"URL":"https:\/\/doi.org\/10.3389\/fphar.2022.863403","relation":{},"ISSN":["1663-9812"],"issn-type":[{"value":"1663-9812","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,31]]},"article-number":"863403"}}