{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:23:41Z","timestamp":1787955821214,"version":"build-2784847793"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate the brucine anti-ulcer effect. Brucine has binding energy values ranging from \u22122.99 to \u22128.11\u00a0kcal\/mol against chosen targets, according to <jats:italic>in silico<\/jats:italic> research. Brucine exhibits an inhibitory effect against <jats:italic>Helicobacter pylori<\/jats:italic>. <jats:italic>In vivo<\/jats:italic> findings revealed that brucine (3\u00a0mg\/kg) showed effective results in healing ethanol-induced ulcer lesions of the gastric region in rats. Brucine showed an inhibitory effect against H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase. Levels of glutathione, glutathione-s-transferase, and catalase were enhanced in the gastric rat tissue with the use of brucine, while a significant decrease in lipid peroxide levels was seen. Histopathological evaluation showed improvement in cellular architecture and a decrease in inflammatory indicators like cyclooxygenase, tumor necrosis factor, and nuclear factor kappa B expression, validated through immunohistochemistry, enzyme-linked immunosorbent assay, and Western blot techniques. In the reverse transcription\u2013polymerase chain reaction, brucine decreased H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase mRNA levels. This study reveals that brucine possesses stable binding affinities against selected targets. Brucine exhibits an anti-ulcer effect, mediated <jats:italic>via<\/jats:italic> anti-<jats:italic>H. pylori<\/jats:italic>, H<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>-ATPase inhibition, and antioxidant and anti-inflammatory pathways.<\/jats:p>","DOI":"10.3389\/fphar.2022.886433","type":"journal-article","created":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T13:51:57Z","timestamp":1660744317000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["Pharmacological investigation of brucine anti-ulcer potential"],"prefix":"10.3389","volume":"13","author":[{"given":"Muhammad","family":"Noman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelum Gul","family":"Qazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najeeb Ur","family":"Rehman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arif-ullah","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2147\/JEP.S125383","article-title":"Evaluation of anti-ulcer activity of the leaf extract of Osyris Quadripartita Decne (Santalaceae) in rats","volume":"9","author":"Abebaw","year":"2017","journal-title":"J. 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