{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:47:41Z","timestamp":1773298061558,"version":"3.50.1"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T00:00:00Z","timestamp":1658102400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Hypoxic\u2013ischemic (HI) insults have important deleterious consequences in newborns, including short-term morbidity with neuromotor and cognitive disturbances. Cannabidiol (CBD) has demonstrated robust neuroprotective effects and shows anxiolytic\/antidepressant effects as well. These effects are thought to be related to serotonin 5-HT<jats:sub>1A<\/jats:sub> receptor (5HT<jats:sub>1A<\/jats:sub>R) activation. We hereby aimed to study the role of 5HT<jats:sub>1A<\/jats:sub>R in the neuroprotective and behavioral effects of CBD in HI newborn piglets.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> 1-day-old piglets submitted to 30\u00a0min of hypoxia (FiO2 10%) and bilateral carotid occlusion were then treated daily with vehicle, CBD 1\u00a0mg\/kg, or CBD with the 5HT<jats:sub>1A<\/jats:sub>R antagonist WAY 100635 1\u00a0mg\/kg 72\u00a0h post-HI piglets were studied using amplitude-integrated EEG to detect seizures and a neurobehavioral test to detect neuromotor impairments. In addition, behavioral performance including social interaction, playful activity, hyperlocomotion, and motionless periods was assessed. Then, brain damage was assessed using histology (Nissl and TUNEL staining) and biochemistry (proton magnetic resonance spectroscopy studies.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> HI led to brain damage as assessed by histologic and biochemistry studies, associated with neuromotor impairment and increased seizures. These effects were not observed in HI piglets treated with CBD. These beneficial effects of CBD were not reversed by the 5HT<jats:sub>1A<\/jats:sub>R antagonist, which is in contrast with previous studies demonstrating that 5HT<jats:sub>1A<\/jats:sub>R antagonists eliminated CBD neuroprotection as assessed 6\u00a0h after HI in piglets. HI led to mood disturbances, with decreased social interaction and playfulness and increased hyperlocomotion. Mood disturbances were not observed in piglets treated with CBD, but in this case, coadministration of the 5HT<jats:sub>1A<\/jats:sub>R antagonist eliminates the beneficial effects of CBD.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> CBD prevented HI-induced mood disturbances in newborn piglets by acting on 5HT<jats:sub>1A<\/jats:sub>R. However, 5HT<jats:sub>1A<\/jats:sub>R activation seems to be necessary for CBD neuroprotection only in the first hours after HI.<\/jats:p>","DOI":"10.3389\/fphar.2022.925740","type":"journal-article","created":{"date-parts":[[2022,7,18]],"date-time":"2022-07-18T15:38:55Z","timestamp":1658158735000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Role of 5HT1A Receptors in the Neuroprotective and Behavioral Effects of Cannabidiol in Hypoxic\u2013Ischemic Newborn Piglets"],"prefix":"10.3389","volume":"13","author":[{"given":"Lorena","family":"Barata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"de Hoz-Rivera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Mart\u00ednez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Villa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia","family":"Campa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Jim\u00e9nez-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Mart\u00ednez-Orgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,7,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"135594","DOI":"10.1016\/j.neulet.2020.135594","article-title":"Chronic Administration of Synthetic Cannabidiol Induces Antidepressant Effects Involving Modulation of Serotonin and Noradrenaline Levels in the hippocampus","volume":"744","author":"Abame","year":"2021","journal-title":"Neurosci. 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