{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:40:42Z","timestamp":1791916842143,"version":"4.3.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T00:00:00Z","timestamp":1682467200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Oligoasthenozoospermia is an important factor leading to male infertility. Yangjing capsule (YC), a traditional Chinese preparation, displays beneficial effects on male infertility. However, whether YC could improve oligoasthenozoospermia remains unclear.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In this study, we aimed to explore the effect of YC in the treatment of oligoasthenozoospermia. Male Sprague-Dawley (SD) rats were treated with 800\u00a0mg\/kg ornidazole once daily for 30\u00a0days to induce <jats:italic>in vivo<\/jats:italic> oligoasthenozoospermia; primary Sertoli cells were treated with 400\u00a0\u03bcg\/mL ornidazole for 24\u00a0h to induce <jats:italic>in vitro<\/jats:italic> oligoasthenozoospermia.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> We found that YC improved the testicle and epididymis weight, sperm concentration, sperm progressive motility, serum testosterone, fertility rate and testis morphology in ornidazole-exposed rats and enhanced cell survival in ornidazole-stimulated primary Sertoli cells. YC also inhibited the ornidazole-caused decrease in nitric oxide (NO) generation and the phosphorylation of phospholipase C \u03b31 (PLC\u03b31), AKT, and eNOS <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> in oligoasthenozoospermia. Furthermore, the knockdown of PLC\u03b31 blunted the beneficial effects of YC <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Collectively, our data suggested that YC protected against oligoasthenozoospermia by promoting NO levels through the PLC\u03b31\/AKT\/eNOS pathway.<\/jats:p>","DOI":"10.3389\/fphar.2023.1056091","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T04:42:59Z","timestamp":1682484179000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Yangjing capsule improves oligoasthenozoospermia by promoting nitric oxide production through PLC\u03b31\/AKT\/eNOS pathway"],"prefix":"10.3389","volume":"14","author":[{"given":"Weimin","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofang","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"153035","DOI":"10.1016\/j.phymed.2019.153035","article-title":"Promoting effect of baicalin on nitric oxide production in CMECs via activating the PI3K-AKT-eNOS pathway attenuates myocardial ischemia-reperfusion injury","volume":"63","author":"Bai","year":"2019","journal-title":"Phytomedicine"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2220","DOI":"10.1038\/s41598-020-80780-6","article-title":"Network pharmacology integrated molecular docking reveals the bioactive components and potential targets of Morinda officinalis-Lycium barbarum coupled-herbs against oligoasthenozoospermia","volume":"11","author":"Bai","year":"2021","journal-title":"Sci. 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