{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:32:25Z","timestamp":1785814345260,"version":"3.56.0"},"reference-count":96,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p>Efforts to develop new artemisinin triple combination therapies effective against artemisinin-tolerant strains of <jats:italic>Plasmodium falciparum<\/jats:italic> based on rational combinations comprising artemisone or other amino-artemisinins, a redox active drug and a third drug with a different mode of action have now been extended to evaluation of three potential redox partners. These are the diethyl analogue AD01 of methylene blue (MB), the benzo [\u03b1]phenoxazine PhX6, and the thiosemicarbazone DpNEt. IC<jats:sub>50<\/jats:sub> values <jats:italic>in vitro<\/jats:italic> against CQ-sensitive and resistant <jats:italic>P. falciparum<\/jats:italic> strains ranged from 11.9\u00a0nM for AD01\u201341.8\u00a0nM for PhX6. PhX6 possessed the most favourable pharmacokinetic (PK) profile: intrinsic clearance rate CL<jats:sub>int<\/jats:sub> was 21.47 \u00b1 1.76\u00a0mL\/min\/kg, bioavailability was 60% and half-life was 7.96\u00a0h. AD01 presented weaker, but manageable pharmacokinetic properties with a rapid CL<jats:sub>int<\/jats:sub> of 74.41 \u00b1 6.68\u00a0mL\/min\/kg leading to a half-life of 2.51 \u00b1 0.07\u00a0h and bioavailability of 15%. DpNEt exhibited a half-life of 1.12\u00a0h and bioavailability of 8%, data which discourage its further examination, despite a low CL<jats:sub>int<\/jats:sub> of 10.20\u00a0mL\/min\/kg and a high C<jats:sub>max<\/jats:sub> of 6.32\u00a0\u00b5M. Efficacies of AD01 and PhX6 were enhanced synergistically when each was paired with artemisone against asexual blood stages of <jats:italic>P. falciparum<\/jats:italic> NF54 <jats:italic>in vitro<\/jats:italic>. The favourable pharmacokinetics of PhX6 indicate this is the best partner among the compounds examined thus far for artemisone. Future work will focus on extending the drug combination studies to artemiside <jats:italic>in vitro<\/jats:italic>, and conducting efficacy studies <jats:italic>in vivo<\/jats:italic> for artemisone with each of PhX6 and the related benzo[\u03b1]phenoxazine SSJ-183.<\/jats:p>","DOI":"10.3389\/fphar.2023.1308400","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T05:05:55Z","timestamp":1704690355000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficacies and ADME properties of redox active methylene blue and phenoxazine analogues for use in new antimalarial triple drug combinations with amino-artemisinins"],"prefix":"10.3389","volume":"14","author":[{"given":"Daniel J.","family":"Watson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizahn","family":"Laing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacobus P.","family":"Petzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Ning","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Parkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubbe","family":"Wiesner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard K.","family":"Haynes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"E1214","DOI":"10.1073\/pnas.1112037108","article-title":"Quantitative assessment of Plasmodium falciparum sexual development reveals potent transmission-blocking activity by methylene blue","volume":"108","author":"Adjalley","year":"2011","journal-title":"Proc. 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