{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T17:27:26Z","timestamp":1787678846467,"version":"build-2784847793"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T00:00:00Z","timestamp":1713484800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec><jats:title>Background and aims:<\/jats:title><jats:p>To investigate mechanisms underlying the effects of Da-Cheng-Qi decoction (DCQD) on severe acute pancreatitis (SAP) capillary leakage syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>In this study, a SAP rat model was established using retrograde perfusion of 5% sodium taurocholate into the biliopancreatic duct. The study included three randomized groups: control, SAP (modeling), and DCQD (via gavage at 2\u00a0h pre-modeling and 2 and 4\u00a0h post-modeling). HPLC was used to analyzed major components of DCQD. Pathological changes and capillary permeability in the rat pancreatic tissues were examined. mRNA levels of claudin 5, occludin, zonula occludin-1 (ZO-1), and junctional adhesion molecules (JAM-C) were assessed using qRT-PCR. Tight junction-associated protein expression was evaluated using immunofluorescence and Western blot analyses. Human umbilical vein endothelial cells (HUVECs) were used to investigate the mechanism m of DCQD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Serum levels of amylase, TNF\u2013\u03b1, IL-1\u03b2, IL-2, and IL-6 were higher in the SAP group compared to the DCQD group (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). DCQD treatment significantly attenuated rat pancreas damage (<jats:italic>p<\/jats:italic> &amp;lt; 0.05) and reduced tissue capillary permeability compared to the SAP group (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Claudin 5, occludin, and ZO-1 expression in the rat tissues was upregulated, but JAM-C was downregulated by DCQD treatment (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). HUVEC permeability was improved by DCQD in a dose-time-dependent manner compared to the SAP group (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). DCQD also upregulated claudin 5, occludin, and ZO-1 expression <jats:italic>in vitro<\/jats:italic> (<jats:italic>p<\/jats:italic> &amp;lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>DCQD can improve capillary permeability in both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> models of SAP by upregulating expression of claudin 5, occludin, and ZO-1, but not JAM-C.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fphar.2024.1138251","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T04:39:00Z","timestamp":1713501540000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Da-Cheng-Qi decoction improves severe acute pancreatitis capillary leakage syndrome by regulating tight junction-associated proteins"],"prefix":"10.3389","volume":"15","author":[{"given":"Jiamin","family":"Tu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinping","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyun","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjuan","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyun","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianguo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinji","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honghao","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianxu","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,4,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e47323","DOI":"10.1371\/journal.pone.0047323","article-title":"Influenza infects lung microvascular endothelium leading to microvascular leak: role of apoptosis and claudin-5","volume":"7","author":"Armstrong","year":"2012","journal-title":"PLoS One"},{"key":"B2","doi-asserted-by":"publisher","first-page":"a029314","DOI":"10.1101\/cshperspect.a029314","article-title":"Cell biology of tight junction barrier regulation and mucosal disease","volume":"10","author":"Buckley","year":"2018","journal-title":"Cold Spring Harb. 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