{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T23:25:51Z","timestamp":1786317951718,"version":"build-2736575974"},"reference-count":40,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021175","name":"LiShui People\u2019s Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021175","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Natural plant-based medicines have gained popularity recently as a major source of inventive, risk-free, and more potent secondary bioactive compounds with medicinal potential. <jats:italic>Catharanthus ovalis<\/jats:italic> is a perennial shrub containing various indole alkaloids cultivated extensively for local medical purposes.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> This research is conducted to identify the phytocompounds present in the leaves of <jats:italic>C. ovalis<\/jats:italic> and its central and peripheral analgesic, thrombolytic, and membrane-stabilizing activities through tail immersion, acetic acid-induced writhing, human blood clot lysis, and erythrocyte lysis by heat and hypotonic solution methods, respectively.<\/jats:p><jats:p><jats:bold>Results and discussion:<\/jats:bold> A total of 39 compounds were identified using GC\u2013MS\/MS techniques, including hexadecanoic acid, methyl ester (56.749%), methyl stearate (29.782%), carvacrol and its TBDMS derivative (12.586%), and 9-octadecenoic acid, methyl ester, (E)-] (9.297%) presented in high quantity. The highest tail immersion latency was observed for the 600\u00a0mg\/kg extract of <jats:italic>C. ovalis<\/jats:italic> crude extract. Both 400 and 600\u00a0mg\/kg doses of <jats:italic>C. ovalis<\/jats:italic> crude extract exhibited prominent peripheral analgesic activity. The maximum thrombolytic effect was observed by DCM soluble fraction extract by inhibiting 54.87% of the clot. However, the aqueous-soluble fraction of this extract manifested an excellent membrane-stabilizing effect by showing 73.98% and 87.51% hemolysis against heat- and hypotonic-induced hemolysis, respectively. Some of the compounds were identified as active agents against different receptors related to these diseases, which supported the findings of <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> tests.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Further investigation needs to be conducted to specify and identify the exact mechanism of action of these compounds.<\/jats:p>","DOI":"10.3389\/fphar.2024.1347069","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T04:56:36Z","timestamp":1709268996000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":23,"title":["Chemico-pharmacological evaluation of the methanolic leaf extract of Catharanthus ovalis: GC\u2013MS\/MS, in vivo, in vitro, and in silico approaches"],"prefix":"10.3389","volume":"15","author":[{"given":"Saimon","family":"Shahriar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia Akter","family":"Shermin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasin","family":"Hasnat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aixia","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwu","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safaet","family":"Alam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah Al","family":"Mamun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,3,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"879","DOI":"10.13005\/bbra\/1211","article-title":"In vitro antifungal potentials of bioactive compound methyl ester of hexadecanoic acid isolated from Annona muricata Linn. 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