{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T16:09:32Z","timestamp":1791648572188,"version":"4.3.4"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Scutellarein (Scu) is a natural occurring flavonoid found in multiple traditional Chinese medicines such as\n                      <jats:italic>Oroxylum<\/jats:italic>\n                      indicum (L.) Kurz and\n                      <jats:italic>Scutellaria baicalensis<\/jats:italic>\n                      , with various pharmacological activities including anti-inflammation, anti-oxidation and myocardial protection. Here, we investigated the therapeutic efficacy of Scu on ulcerative colitis (UC) and the underlying mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Efficacy of Scu on UC was evaluated in dextran sulfate sodium (DSS) induced colitis mouse model. Inflammation in colonic tissues was assessed by myeloperoxidase activity assay and RT-qPCR. Barrier proteins expression was examined using immunostaining and Western blot. IL-1\u03b2-treated HT-29 cells was used for mechanical investigation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Gavage of Scu significantly decreased the DAI score, improved colon shortening, ameliorated the pathological score in DSS-treated mice with better efficacy than the positive drug, 5-aminosalicylic acid. Scu also inhibited the expression levels of cytokines (\n                      <jats:italic>Il-1\u03b2<\/jats:italic>\n                      ,\n                      <jats:italic>Tnf-\u03b1<\/jats:italic>\n                      ,\n                      <jats:italic>Il-1\u03b1<\/jats:italic>\n                      ,\n                      <jats:italic>Il-6<\/jats:italic>\n                      , and\n                      <jats:italic>Cxcl1<\/jats:italic>\n                      ) as well as barrier proteins (E-cadherin, Occludin, and ZO-1) in colon tissues of DSS mice. In intestinal epithelial HT-29 cells, Scu attenuated the IL-1\u03b2-downregulated expression levels of E-cadherin, occludin, and ZO-1, while reduced IL-1\u03b2-upregulated\n                      <jats:italic>IL-6<\/jats:italic>\n                      and\n                      <jats:italic>IL-8<\/jats:italic>\n                      mRNA levels. Moreover, Scu inhibited the phosphorylation and nuclear translocation of NF-\u03baB and suppression of NF-\u03baB phosphorylation abolished IL-1\u03b2-disrupted epithelial barrier integrity and IL-1\u03b2-upregulated proinflammatory mediators expression in HT-29 cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These data demonstrate that Scu is an efficacious therapeutic agent to treat UC. Inhibition of inflammatory responses and maintenance of epithelial barrier integrity through NF-\u03baB signaling pathway underlines Scu therapeutic effect on UC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2024.1479441","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:40:10Z","timestamp":1729471210000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Scutellarein ameliorates dextran sulfate sodium-induced ulcerative colitis by inhibiting colonic epithelial cell proinflammation and barrier disruption"],"prefix":"10.3389","volume":"15","author":[{"given":"Qinglian","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haidong","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaowen","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyu","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.3390\/pharmaceutics14061183","article-title":"Multi-strain-probiotic-loaded nanoparticles reduced colon inflammation and orchestrated the expressions of tight junction, NLRP3 inflammasome and caspase-1 genes in DSS-induced colitis model","volume":"14","author":"Alkushi","year":"2022","journal-title":"Pharmaceutics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"5653","DOI":"10.4049\/jimmunol.180.8.5653","article-title":"Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability","volume":"180","author":"Al-Sadi","year":"2008","journal-title":"J. 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