{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:14:21Z","timestamp":1753892061752,"version":"3.41.2"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009391","name":"Kafkas \u00dcniversitesi","doi-asserted-by":"publisher","award":["2021-TS-70"],"award-info":[{"award-number":["2021-TS-70"]}],"id":[{"id":"10.13039\/501100009391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim was to investigate the possible protective effect of <jats:italic>Berberis vulgaris<\/jats:italic> extract, which has anti-inflammatory and antioxidant properties, in the testes in an ischemia\u2013reperfusion (IR) injury model by utilizing molecular, biochemical, histopathological, and immunohistochemical methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 56 male rats were divided in to 7 equal groups. Ischemia was induced by taking the testicles out of the scrotum and rotating them 720\u00b0 and after 3\u00a0h reperfusion was induced. <jats:italic>Berberis vulgaris<\/jats:italic> extract was administered 3\u00a0h before ischemia. The reperfusion groups were treated by oral gavage 1\u00a0h before reperfusion. Following the 3-h reperfusion period, tissue and blood samples were collected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histopathologically, H&amp;amp;E staining showed disruption in the seminiferous tubule structure in the ischemia and IR groups SOD levels decreased significantly in the IR group compared to the control group, whereas Berberis vulgaris did not change SOD levels following IR at 300 and 600\u00a0mg\/kg doses. At the dose of 600\u00a0mg\/kg, <jats:italic>Berberis vulgaris<\/jats:italic> significantly increased SOD levels compared to the ischemia group CAT activity was significantly higher in the BV300 and BV600 groups compared to the ischemia group. CAT activity was significantly lower in the IR group compared to the ischemia group (p &amp;lt; 0.001). When compared to the control group, the ischemia group had a roughly 3-fold increase in caspase-3 expression. In the IR group, this ratio was dramatically increased\u2014roughly 5-fold. However, the antiapoptotic gene Bcl-2 expression was significantly decreased in both the ischemia and IR groups compared to the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>B. vulgaris<\/jats:italic> plant extract may have a protective effect against testes IR injury. Further molecular studies are needed to clarify this protective effect.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fphar.2025.1514676","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T06:16:03Z","timestamp":1743488163000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Berberis vulgaris fruit extract mitigated apoptosis in experimentally induced testicular ischemia and reperfusion injury in rats"],"prefix":"10.3389","volume":"16","author":[{"given":"Serdar","family":"Yigit","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakir","family":"Akgun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isa","family":"Yesilyurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soner","family":"Bitiktas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ergin","family":"Taskin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Alper Kahraman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammed","family":"Yayla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P\u0131nar Aksu","family":"Kilicle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyit","family":"Ali Bingol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengul","family":"Ozdemir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcl Esma","family":"Akdogan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Ali","family":"Karag\u00f6z","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"503","DOI":"10.22038\/IJBMS.2017.8674","article-title":"Berberis vulgaris for cardiovascular disorders: a scoping literature review","volume":"20","author":"Abushouk","year":"2017","journal-title":"Iran. 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