{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:03:10Z","timestamp":1790719390359,"version":"4.1.0"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Plants like <jats:italic>Solanum dasyphyllum<\/jats:italic> and <jats:italic>Dovyalis abyssinica<\/jats:italic> have been traditionally used to treat ailments such as fevers, asthma, high blood pressure, infections, stomachaches, skin rashes, for their blood-cleansing and anti-venom properties. Despite their traditional use, limited scientific studies have explored their pharmacological potential, particularly their antimicrobial and anticancer properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>Purpose<\/jats:title><jats:p>This study evaluated <jats:italic>in vitro<\/jats:italic> antimicrobial and cytotoxic activities of methanolic extracts from <jats:italic>Solanum dasyphyllum<\/jats:italic> (root, stem, and leaf) and <jats:italic>Dovyalis abyssinica<\/jats:italic> (root, stem).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Solanum dasyphyllum<\/jats:italic> and <jats:italic>Dovyalis abyssinica<\/jats:italic> samples were defatted using <jats:italic>n<\/jats:italic>-hexane and subsequently macerated in methanol to obtain crude extracts. Antimicrobial activity was assessed using agar well diffusion and micro-broth dilution methods, while cytotoxicity against the MCF-7 breast cancer cell line was evaluated using the MTT assay. Quantitative analyses of total phenolic and flavonoid contents were carried out using standard spectrophotometric assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both plant extracts showed strong antibacterial activity, with MIC values ranging from 0.195 to 3.125\u00a0mg\/mL against all tested strains. <jats:italic>S. epidermidis, E. coli<\/jats:italic>, and <jats:italic>P. aeruginosa<\/jats:italic> were especially susceptible, with MICs as low as 0.195\u00a0mg\/mL for <jats:italic>Solanum dasyphyllum<\/jats:italic> root and stem and <jats:italic>Dovyalis abyssinica<\/jats:italic> root extracts. The <jats:italic>Solanum dasyphyllum<\/jats:italic> root extract also exhibited antifungal activity against <jats:italic>T. rubrum<\/jats:italic> (inhibition zone 20.67\u00a0mm). The root extract of <jats:italic>Dovyalis abyssinica<\/jats:italic> showed the highest cytotoxicity against MCF-7 breast cancer cells (IC<jats:sub>50<\/jats:sub> = 0.67\u00a0\u03bcg\/mL), outperforming doxorubicin (IC<jats:sub>50<\/jats:sub> = 3.43\u00a0\u03bcg\/mL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The methanolic extracts of both plants exhibited significant <jats:italic>in vitro<\/jats:italic> antibacterial, antifungal, and cytotoxic activities. Notably, the root extracts of both <jats:italic>Solanum dasyphyllum<\/jats:italic> and <jats:italic>Dovyalis abyssinica<\/jats:italic>, along with the stem extract of <jats:italic>S. dasyphyllum<\/jats:italic>, demonstrated strong antibacterial effects. The root extract of <jats:italic>D. abyssinica<\/jats:italic> showed remarkable cytotoxicity against MCF-7 breast cancer cells (IC<jats:sub>50<\/jats:sub> = 0.67\u00a0\u03bcg\/mL), outperforming the standard chemotherapeutic agent doxorubicin. These findings highlight its potential as a promising lead for anticancer drug development. Further studies are warranted to isolate the active constituents and confirm their therapeutic efficacy through <jats:italic>in vivo<\/jats:italic> investigations and clinical trials.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fphar.2025.1529854","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T15:13:31Z","timestamp":1750950811000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["In vitro antimicrobial and cytotoxic evaluation of leaf, root, and stem extracts of Solanum dasyphyllum and root and stem extracts of Dovyalis abyssinica"],"prefix":"10.3389","volume":"16","author":[{"given":"Dereilo Bekere","family":"Belitibo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asfaw","family":"Meressa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temesgen","family":"Negassa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abiy","family":"Abebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sileshi","family":"Degu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milkyas","family":"Endale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frehiwot Teka","family":"Assamo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temesgen Abdisa","family":"Ayana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Getahun Tadesse","family":"Gurmessa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negera","family":"Abdissa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1589877","DOI":"10.1155\/2022\/1589877","article-title":"Traditional and phytochemical bases of herbs, shrubs, climbers, and trees from Ethiopia for their anticancer response","volume":"2022","author":"Abate","year":"2022","journal-title":"Biomed. 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