{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:59:50Z","timestamp":1790323190539,"version":"4.1.0"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T00:00:00Z","timestamp":1745971200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Pharmacol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Resveratrol, a naturally occurring polyphenolic compound found in grapes, berries, and traditional medicinal plants like Polygonum cuspidatum, has been used for centuries in traditional medicine systems for its anti-inflammatory, antioxidant, and cardioprotective properties. Ulcerative colitis (UC), a chronic inflammatory bowel disease, is characterized by intestinal barrier disruption due to excessive colonocyte apoptosis, leading to increased permeability and inflammation. Targeting apoptosis is a critical therapeutic strategy for UC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim of the study<\/jats:title>\n                    <jats:p>\n                      This study aims to investigate the therapeutic potential of Resveratrol in ulcerative colitis (UC) by targeting excessive colonocyte apoptosis and intestinal barrier dysfunction. Specifically, we seek to elucidate the mechanisms through which Resveratrol modulates apoptosis-related pathways and evaluate its efficacy in restoring intestinal homeostasis and mitigating UC progression in both\n                      <jats:italic>in vivo<\/jats:italic>\n                      and\n                      <jats:italic>in vitro<\/jats:italic>\n                      models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We used dextran sulfate sodium (DSS) to induce UC in a mouse model. Colonic damage was assessed through colonic length measurement, histological examination, and immunofluorescence staining. Single-cell sequencing was employed to explore changes in the colonic immune microenvironment and cellular signaling pathways after Resveratrol treatment.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , colonocytes isolated from healthy mouse colonic tissue were exposed to TGF-\u03b2 to induce apoptosis. DNA fragmentation, mitochondrial membrane potential, and annexin V\/propidium iodide staining were used to assess apoptosis. Additionally, we employed an Adeno-Associated Virus system to overexpress MDM2 in the colon and evaluate its protective role in DSS-induced UC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Resveratrol treatment effectively repaired colonic damage in the UC mouse model by significantly increasing colon length, reducing inflammatory cell infiltration, and mitigating mucosal injury. Single-cell sequencing revealed that Resveratrol primarily targeted colonocytes, decreasing genes related to apoptosis and the P53 pathway.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , Resveratrol reduced DNA fragmentation, apoptotic cell populations, and increased mitochondrial membrane potential in a dose-dependent manner. Furthermore, Resveratrol increased MDM2 expression, inhibiting P53 and downstream pro-apoptotic signaling. Nutlin-3a, an MDM2 inhibitor, reversed the anti-apoptotic effects of Resveratrol. Overexpression of MDM2 in the colon protected against DSS-induced damage.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Resveratrol is an effective treatment for DSS-induced UC, primarily by inhibiting excessive apoptosis in colonocytes through the MDM2\/P53\/PUMA axis. MDM2 presents a promising therapeutic target for UC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fphar.2025.1572906","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T01:42:48Z","timestamp":1745977368000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Resveratrol targeting MDM2\/P53\/PUMA axis to inhibit colonocyte apoptosis in DSS-induced ulcerative colitis mice"],"prefix":"10.3389","volume":"16","author":[{"given":"Rui","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Pathology, Yaan People\u2019s Hospital","place":["Yaan, China"]},{"name":"School of Basic Medical Sciences, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Management, the Affiliated Hospital of Southwest Medical University","place":["Luzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyuan","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengyu","family":"Miao","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Southwest Medical University","place":["Luzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Health Technology and Informatics, The Hong Kong Polytechnic University","place":["Kowloon, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Yaan People\u2019s Hospital","place":["Yaan, China"]},{"name":"Department of Pathology, The Affiliated Hospital, Southwest Medical University","place":["Luzhou, China"]},{"name":"Precision Medicine Center, Yaan People\u2019s Hospital","place":["Yaan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,4,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1186\/s12935-021-02280-5","article-title":"Resveratrol mediates its anti-cancer effects by Nrf2 signaling pathway activation","volume":"21","author":"Alavi","year":"2021","journal-title":"Cancer cell Int."},{"key":"B2","doi-asserted-by":"publisher","first-page":"122027","DOI":"10.1016\/j.ijpharm.2022.122027","article-title":"Co-Delivery of erlotinib and resveratrol via nanostructured lipid Carriers: a synergistically promising approach for cell proliferation prevention and ROS-Mediated apoptosis activation","volume":"624","author":"Asadollahi","year":"2022","journal-title":"Int. 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